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Anti-double-stranded DNA antibody IgG chemiluminiscence immunoassay kit and preparation method thereof, and application of anti-double-stranded DNA antibody IgG chemiluminiscence immunoassay kit

A chemiluminescence immunity and chemiluminescence technology, which is applied in the direction of chemiluminescence/bioluminescence, analysis through chemical reaction of materials, biological testing, etc. Complicated operation and other issues, to achieve the effect of improving sensitivity, increasing luminous intensity and duration, and wide linear range

Pending Publication Date: 2020-02-28
南京迪安医学检验所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In this field, the common method for detecting anti-double-stranded DNA antibody IgG is enzyme-linked immunosorbent assay, but it has some shortcomings, such as the use of many reagents, complicated operation, and easy to cause cross-contamination between various reagents. Affect the accuracy of the test results, use manual operation, the amount of reagent or sample taken is not very accurate, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] In this embodiment, an anti-double-stranded DNA antibody IgG chemiluminescent immunoassay kit is provided, which includes biotinylated double-stranded DNA antigen, streptavidin-coated nano-magnetic microspheres, Milo-labeled mouse anti-human IgG monoclonal antibody, chemiluminescent marker, chemiluminescent substrate solution, anti-double-stranded DNA antibody IgG calibrator;

[0030] The chemiluminescent marker is isoluminol; the nanomagnetic microspheres are citric acid-modified Fe 3 o 4 The particle diameter of the nano-magnetic microspheres is 50-500nm, and the chemiluminescent substrate solution includes a hydrogen peroxide solution with a mass percentage concentration of 0.1% and a p-phenol solution with a mass percentage concentration of 0.05%.

[0031] The preparation method of each component in the kit is as follows:

[0032] (1) Preparation of biotinylated double-stranded DNA antigen: In a dark room, mix dsDNA and photosensitive biotin in proportion, irradia...

Embodiment 2

[0038] In this embodiment, an anti-double-stranded DNA antibody IgG chemiluminescent immunoassay kit is provided, which includes biotinylated double-stranded DNA antigen, streptavidin-coated nano-magnetic microspheres, Milo-labeled mouse anti-human IgG monoclonal antibody, chemiluminescent marker, chemiluminescent substrate solution, anti-double-stranded DNA antibody IgG calibrator;

[0039] The chemiluminescent marker is isoluminol isothiocyanate; the nanomagnetic microspheres are citric acid-modified Fe 3 o 4 The particle size of the nano magnetic microspheres is 50-500nm, and the chemiluminescence substrate solution includes a hydrogen peroxide solution with a mass percentage concentration of 0.2% and a p-phenol solution with a mass percentage concentration of 0.1%.

[0040] Its preparation method is identical with embodiment 1.

Embodiment 3

[0042] In this embodiment, an anti-double-stranded DNA antibody IgG chemiluminescent immunoassay kit is provided, which includes biotinylated double-stranded DNA antigen, streptavidin-coated nano-magnetic microspheres, Milo-labeled mouse anti-human IgG monoclonal antibody, chemiluminescent marker, chemiluminescent substrate solution, anti-double-stranded DNA antibody IgG calibrator;

[0043] The chemiluminescent marker is isoluminol; the nanomagnetic microspheres are citric acid-modified Fe 3 o 4 The particle size of the nano magnetic microspheres is 50-500nm, and the chemiluminescent substrate solution includes a hydrogen peroxide solution with a mass percentage concentration of 0.005% and a p-phenol solution with a mass percentage concentration of 0.05%.

[0044] Its preparation method is identical with embodiment 1.

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Abstract

The invention provides an anti-double-stranded DNA antibody IgG chemiluminiscence immunoassay kit and preparation method thereof, and application of an anti-double-stranded DNA antibody IgG chemiluminiscence immunoassay kit. The kit comprises a biotinylated double-stranded DNA antigen, streptavidin-coated nano magnetic microspheres, an isoluminol-labeled mouse anti-human IgG monoclonal antibody, achemiluminiscence marker, a chemiluminiscence substrate solution and an anti-double-stranded DNA antibody IgG calibration product. The chemiluminiscence marker is isolumilol or isothiocyanate isolumilol; and the nano magnetic microspheres are nano magnetic microspheres. The anti-double-stranded DNA antibody IgG chemiluminiscence immunoassay kit is used for antibody detection, the sensitivity is high, the reproducibility is good, the accuracy is high, the cost is low, and the operation is simple.

Description

technical field [0001] The invention belongs to the technical field of immunoassay, and relates to an anti-double-stranded DNA antibody IgG chemiluminescent immunoassay kit and a preparation method and application thereof. Background technique [0002] Double-stranded deoxyribonucleic acid (double stranded DNA, dsDNA) antibody is an autoantibody that can bind to natural DNA. This antibody exists in the serum of most patients with systemic lupus erythematosus (SLE), and it is closely related to the pathological changes, activity, curative effect and prognosis of SLE. [0003] Therefore, the detection of anti-double-stranded DNA antibody IgG is of great significance for SLE diagnosis, disease tracking and prognosis judgment. [0004] In this field, the common method for detecting anti-double-stranded DNA antibody IgG is enzyme-linked immunosorbent assay, but it has some shortcomings, such as the use of many reagents, complicated operation, and easy to cause cross-contaminatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N21/76
CPCG01N33/5308G01N21/76
Inventor 冯涛陈国动周慧张楠朱红甜
Owner 南京迪安医学检验所有限公司
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