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Breeding method and application of high-linoleic-acid maize inbred line

A high linoleic acid, inbred line technology, applied in the directions of application, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of not significantly increasing the proportion of linoleic acid, high oil corn not being widely promoted and applied, etc. , to achieve the effect of improving economic efficiency

Inactive Publication Date: 2020-02-28
SUQIAN AGRI SCI RES INST JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although high-oil (high-fatty acid) corn greatly improves the total energy level of corn grains, improves many of its nutritional components, and significantly increases the total fatty acid content of corn grains. The absolute content is higher than that of ordinary corn, but the proportion of linoleic acid in the total fatty acid has not been significantly increased, and because of various limiting factors, high-oil corn has not been widely used

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) F1 was obtained by crossing Suyu 20 and Yunrui 21, which was named SY12-1; the introduced inbred line K12 with compact plant type, good sex coordination, long ear, hard grain type and good resistance was used as the female parent , medium-short-stalk disease-resistant and large-ear hybrid SY12-1 was used as the male parent, and 2 ears of the hybrid F1 were obtained to make the S0 generation of the basic population;

[0034] 2) The harvested basic seeds of the S0 generation were irradiated with 60Co-γ rays with an irradiation dose of 200Gy and a dose rate of 1.52Gy / min for mutagenesis to obtain a mutagenic population;

[0035] 3) All the irradiated seeds are planted, selfed as much as possible during the pollination period, and harvested according to single ear numbers during the harvest period to obtain self-crossed S1 generation materials. Take 2-3g of seeds from each ear, and use gas chromatography to select high linoleic acid corn seeds with a linoleic acid conten...

Embodiment 2

[0039] The hybrid combination SQ1817 prepared with YM-2 as the female parent and Chang 7-2 as the male parent has been identified by multiple points in Hainan and Jiangsu. It is resistant to high temperature, rust, and lodging. The growth period is 105 days. Low position, high temperature resistance in loose powder stage, cylindrical ear shape, yellow and semi-hard grain, total fatty acid content of 4.8%, linoleic acid content accounts for 61.5% of total fatty acid on average, which is higher than that of the control Suyu 29 and Zhengdan 958 17.5%, 19.6%, and the yield reached 605.7Kg, which was 2.7% and 4.8% higher than that of the control Suyu 29 and Zhengdan 958, respectively.

[0040] The invention provides a method for breeding a high linoleic acid corn inbred line and its application. The inbred line with a content of linoleic acid in fatty acids exceeding 60% can be bred by using the breeding method, and the quality is good; Using it as a parent, it can also be used to ...

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Abstract

The invention provides a breeding method of a high-linoleic-acid maize inbred line and relates to the technical field of novel corn variety breeding. The breeding method is characterized in that by base population building and radiation mutagenesis which are combined with linoleic acid content detection, the high-linoleic-acid maize inbred line is bred through continuous selfing, and the linoleicacid content in the fatty acid of the offspring of the inbred line is allowed to exceed 60%. Continuous combining ability measurement of S4-S6 generations shows that the screened high-linoleic-acid maize inbred line YM-2 is excellent in comprehensive characters, the linoleic acid content in the fatty acid of the inbred line YM-2 reaches 61%, and the inbred line YM-2 is resistant to high temperature and Puccinia polysora Underw, high in stem rot, ostrinia nubilalis and viral disease resistance, medium in northern leaf blight and southern leaf blight and high in lodging resistance. Accordingly,by using the inbred line as the parent for hybridization, maize hybrids high in linoleic acid content can be cultivated, the nutrient content of common maize can be further increased, the value of thecommon maize serving as maize for feed or maize developed into healthcare food can be increased, and economic benefits can be increased.

Description

technical field [0001] The invention belongs to the technical field of breeding of new corn varieties, and in particular relates to a breeding method of high linoleic acid corn inbred lines. Background technique [0002] Corn kernels contain a variety of fatty acids, of which linoleic acid is the most abundant. Linoleic acid is an unsaturated fatty acid that is necessary for the human body but cannot be synthesized by itself in the body, and must be ingested from food. Many studies have shown that if the human body lacks linoleic acid, it will cause a series of cardiovascular and cerebrovascular diseases, such as high blood pressure, cerebral hemorrhage, heart disease, heart and brain blood thickening, hemiplegia and stroke. And linoleic acid can soften cardiovascular and cerebrovascular, promote blood circulation, reduce fat and blood pressure, promote metabolism, regulate endocrine and delay aging, etc., known as "vascular scavenger". [0003] At present, the content of ...

Claims

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Application Information

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IPC IPC(8): A01H1/04A01H1/02A01H1/06
CPCA01H1/02A01H1/04A01H1/06
Inventor 刘晓飞王卫军崔小平孙宝林
Owner SUQIAN AGRI SCI RES INST JIANGSU ACAD OF AGRI SCI
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