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Flavonoid derivative as well as preparation method and application thereof

A technology of derivatives and flavones, which is applied in the field of flavone derivatives and their preparation, achieving the effect of broad application prospects

Active Publication Date: 2020-02-18
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, the use of microorganisms to biotransform plants or plant extracts to find active new compounds has become a research hotspot at home and abroad, but there is no biotransformation of Sophora japonica using insect symbiotic bacteria as the object to prepare new compounds with anti-phytopathogenic activity. to report

Method used

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  • Flavonoid derivative as well as preparation method and application thereof
  • Flavonoid derivative as well as preparation method and application thereof
  • Flavonoid derivative as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Flavonoid derivatives are prepared by the following steps:

[0039] (1) Carry out strain culture to originating from Chinese rice locust (Oxya chinensis Thunber) endophytic fungus Nigrospora sphaerica (Nigrospora sphaerica) ZMT05 in strain culture medium earlier, used strain culture medium is to contain glucose 1.5% (weight percentage, the same below), yeast extract 0.02%, peptone 0.02%, agar 1.0%, sodium chloride 0.2%, and the rest is water. When used, it is made into a test tube slope, and the above-mentioned strains are cultivated at 28°C for 10 days;

[0040] (2) Carry out fermentation culture to Nigrospora sphaerica (Nigrospora sphaerica) ZMT05 again, in the fermentation medium used, be to contain Sophora japonica 1.0% (percentage by weight, the same below), rice 70%, sodium chloride 1.0% and water balance , and cultured statically at room temperature 25°C for 30 days.

[0041] (3) The strain fermented in step (2) is extracted 2 times with ethanol-water mixed sol...

Embodiment 2

[0043] Flavonoid derivatives are prepared by the following steps:

[0044](1) Carry out strain culture to Chinese rice locust (Oxya chinensis Thunber) endophytic fungus Nigrospora sphaerica (Nigrospora sphaerica) ZMT05 earlier in strain culture medium, used strain culture medium is to contain glucose 3.5% (percentage by weight, The same below), yeast extract 1.5%, peptone 1.5%, agar 2.5%, sodium chloride 2.0%, and the rest is water. When used, it is made into a test tube slant, and the above bacteria are cultured at 28°C for 4 days;

[0045] (2) Carry out fermentation culture to Nigrospora sphaerica (Nigrospora sphaerica) ZMT05 again, the fermentation medium used is to contain Sophora japonica 3.0% (weight percentage, the same below), rice 30.0% (weight percentage, the same below), sodium chloride 3.0% and the balance of water, static culture at 20°C for 60 days;

[0046] (3) The strain fermented in step (2) is leached 3 times with methanol-water mixture (volume ratio is 90:...

Embodiment 3

[0048] Flavonoid derivatives are prepared by the following steps:

[0049] (1) Carry out strain culture to Chinese rice locust (Oxya chinensis Thunber) endophytic fungus Nigrospora sphaerica (Nigrospora sphaerica) ZMT05 earlier in strain culture medium, used strain culture medium is to contain glucose 2.5% (percentage by weight, The same below), yeast extract 0.5%, peptone 1.0%, agar 1.5%, sodium chloride 1.5%, and the rest is water. When used, it is made into a test tube slant, and the above bacteria are cultured at 28°C for 4 days;

[0050] (2) Carry out fermented culture to Nigrospora sphaerica (Nigrospora sphaerica) ZMT05 again, used fermentation medium is to contain Sophora japonica 2.0% (percentage by weight, the same below), rice 50.0%, sodium chloride 2.5% and water surplus, Static culture at 30°C for 45 days;

[0051] (3) The strain fermented in step (2) is leached 4 times with ethanol-water mixed solution (volume ratio is 80:20), after concentrating under reduced p...

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Abstract

The invention discloses a flavonoid derivative as well as a preparation method and application thereof. The flavonoid derivative has a molecular formula of C21H20O6, and has a structural formula of aformula I shown in the description. The preparation method of the flavonoid derivative comprises the following steps: (1) performing bacterium culture on (Nigrospora sphaerica) ZMT05 in a bacterium culture medium; and (2) performing fermentation culture on the bacterium obtained in the step (1) in a fermentation culture medium (containing pagoda tree flowers), performing digestion with an ethanolaqueous solution or a methanol aqueous solution, performing vacuum concentration, further performing extraction by using an organic solvent, and performing further purification through silica gel column chromatography and recrystallization, so as to obtain the flavonoid derivative. The flavonoid derivative disclosed by the invention has good functions of inhibiting citrus penicillium and gloeosporium musarum, can be used as a microbial agricultural antibacterial agent, is feasible in large-scale production of raw materials, and has wide application prospects.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a flavonoid derivative and its preparation method and application. Background technique [0002] Plant-derived natural products are one of the important sources of medicine and pesticides. Sophora japonica is a medium-sized deciduous tree widely distributed in China and other Asian countries. The flower buds of this plant are called Sophora japonica, and as a traditional Chinese medicine, it is used in the folk to treat or assist in the treatment of various diseases, such as high blood pressure, high blood fat, hemorrhoids, blood in the stool, uterine bleeding, etc. Studies on the chemical components of Sophora japonica at home and abroad have found that it mainly contains flavonoids, such as rutin, quercetin, isorhamnetin, genistein, sophoramethin, kaempferol, and isorhamnetin-3-ruta Glycosides, kaempferol-3-rutinoside, etc.; triterpenoid saponins such as red be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C12P17/06A01N43/16A01P3/00C12R1/645
CPCA01N43/16C07D311/30C12P17/06
Inventor 李春远丁唯嘉
Owner SOUTH CHINA AGRI UNIV
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