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Preparation method and application of gold-platinum composite nanometer diagnosis and treatment agent

A nano, chelating agent technology, applied in the field of nanomaterials, can solve the problems of limiting the therapeutic effect, and achieve the effect of overcoming the hypoxic environment of the tumor, enhancing the photodynamic effect, and enhancing the stability.

Active Publication Date: 2020-02-07
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this type of therapy also faces some challenges, such as the tissue penetration depth of light, and hypoxia in the tumor area, all of which limit the therapeutic effect of this type of therapy.

Method used

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  • Preparation method and application of gold-platinum composite nanometer diagnosis and treatment agent
  • Preparation method and application of gold-platinum composite nanometer diagnosis and treatment agent
  • Preparation method and application of gold-platinum composite nanometer diagnosis and treatment agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Synthetic Au 2 Pt nanoparticles: 33mmol L -1 L-proline was dissolved in 10 mL of water. Then add 2mmol L -1 HAuCl 4 and H 2 PtCl 6 The solution was added to the mixture. After adjusting the pH of the solution to 9, add 6.7mmol L dropwise -1 The ascorbic acid solution was stirred at room temperature for 20 minutes, centrifuged at a high speed (14000rpm / min, 5min), washed with deionized water for 3 times, and dispersed in 10mL of water.

[0050] To verify that the synthesized nanoparticles are Au 2 Pt alloy was analyzed by X-ray diffractometer, and the representative diffraction peaks in the X-ray diffraction pattern appeared at 38.34°, 44.44°, 64.86° and 77.88°, which originated from the face-centered cubic (fcc) Au 2 The (111), (200), (220) and (311) planes of the Pt alloy, these peaks are between the elemental Au and the elemental Pt, indicating its alloy properties (attached figure 1 ). Proven successful synthesis of Au 2 Pt nanoparticles. Au can be observ...

Embodiment 2

[0052] Synthetic Au 2 Pt-PEG-Ce6 nanoparticles: 10 mg SH-PEG-NH 2 Added to the solution prepared in Example 1, stirred at room temperature for 24 hours to obtain Au 2 Pt-PEG-NH 2 , high-speed centrifugation, washed 3 times with water, and dispersed in 5mL water.

[0053] Then take 1mg Ce6, 8mg EDC, 12mg NHS dissolved in 5mL DMF solution, react for 1 hour, then add Au 2 Pt-PEG-NH 2 , reacted at room temperature for 24 hours. The precipitate was collected by centrifugation and washed 3 times with water to obtain Au 2 Pt-PEG-Ce6 nanoparticles.

[0054] The results of post-test analysis of the nanoparticles prepared in the above examples are provided below.

[0055] (1) For synthetic Au 2 Pt and Au 2 Hydration particle size analysis of Pt-PEG-Ce6 nanoparticles:

[0056] From 57.03 nm to 70.89 nm as shown in Figure 3.

[0057] For synthetic Au 2 Pt, Au 2 Pt-PEG-NH 2 , Au 2 Zeta potential analysis of Pt-PEG-Ce6 nanoparticles:

[0058] Such as Figure 4 As shown, Au ...

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Abstract

The invention provides a preparation method and application of a gold-platinum composite nanometer diagnosis and treatment agent, and relates to a method for obtaining Au2Pt-PEG-PS (PS: photosensitizer) through the steps of synthesizing Au2Pt nanometer particles by using L-proline as a chelating agent and L-ascorbic acid as a reducing agent and performing post modification, and an application of the Au2Pt-PEG-PS (PS: photosensitizer). The Au2Pt-PEG-PS nanometer particles have catalase activity and peroxidase activity, and can react with hydrogen peroxide (H2O2) overexpressed in tumors under the weak acid condition to produce oxygen (O2) and high-toxicity active oxygen hydroxyl radicals (.OH) in situ, so that the effects of overcoming anoxia, strengthening light power effects and killing cancer cells can be achieved. Au2Pt alloy further can realize multimode imaging of CT imaging, photoacoustic imaging, photothermal imaging and the like at the same time, and united treatment and diagnosis and treatment integration are realized.

Description

technical field [0001] The invention belongs to the field of nanomaterials, in particular to a gold-platinum composite nano-diagnosis and treatment agent Au 2 Preparation method and application of Pt-PEG-Ce6. Background technique [0002] The number of cancer patients in the world is increasing year by year, which seriously threatens people's health. Traditional treatment methods limit the effective treatment of cancer due to their inherent limitations. Photodynamic therapy and photothermal therapy have attracted extensive attention of researchers due to their advantages of minimal invasiveness, low toxicity, and high selectivity. However, this type of therapy also faces some challenges, such as the tissue penetration depth of light and the hypoxia in the tumor area, all of which limit the therapeutic effect of this type of therapy. Therefore, it is particularly important to develop a gold-platinum composite nano-therapeutic agent to overcome tumor hypoxia and to realize a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K49/04A61K49/22A61K41/00A61P35/00
CPCA61K49/0002A61K49/04A61K49/22A61K41/0052A61K41/0071A61P35/00A61K2300/00
Inventor 李春霞王曼吕光磊陈晴王冬梅
Owner ZHEJIANG NORMAL UNIVERSITY
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