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Carp spring viraemia oral vaccine capable of being added into feed

A technology of carp spring viremia and oral vaccine, which is applied in the field of carp disease immunization and prevention, can solve the problems of lag in basic research work, economic losses of farmers, and complexity of breeding environmental factors, and achieves an effect that is conducive to absorption

Inactive Publication Date: 2020-02-04
ZHEJIANG INST OF FRESH WATER FISHERIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the basic research work related to carp virus biology is relatively lagging behind, coupled with the complexity of aquaculture environmental factors, it is impossible to determine the pathogenic mechanism that causes carp spring viremia, resulting in outbreaks of the virus disease from time to time. Huge economic loss to households

Method used

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  • Carp spring viraemia oral vaccine capable of being added into feed
  • Carp spring viraemia oral vaccine capable of being added into feed
  • Carp spring viraemia oral vaccine capable of being added into feed

Examples

Experimental program
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Effect test

Embodiment

[0034] An oral vaccine against carp spring viremia that can be added to feed is prepared through the following steps:

[0035] (1) Cloning and expression of the G protein gene of carp spring viremia virus: the G protein gene was amplified from the preserved SVCV virus by PCR; reaction system: 2×PCR Master Mix 25 μl, 10 μM primers (SVG-F: 5'-CATGCCATGGATGTCTATCATCATCAGCTACATCGCA-3'(SEQ ID No.1), SVG-R:5'-CGAGCTCAACGAAGGACCGCATTTCG-3'(SEQ ID No.2) each 1μl, template 3μl, make up to 50μl with double distilled water; amplification reaction Conditions: pre-denaturation at 95°C for 4min; denaturation at 94°C for 45s, annealing at 55°C for 45s, extension at 72°C for 1min, and 35 cycles at 72°C for 10min). The amplified fragment was cloned into the pMD18-T vector, and then transformed into Escherichia coli DH5α to select positive clone strains. After being identified by Nco I and Sac I double enzyme digestion, it was connected to the intermediate bridge plasmid pET30a-G, and then the...

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Abstract

The invention discloses a carp spring viraemia oral vaccine capable of being added into feed. G protein of a carp spring viraemia virus is displayed on the spore surface by a spore surface display technology, spores of recombinant bacteria are obtained by enrichment culture and spore induction, and a solid recombinant vaccine is prepared by freeze drying. The vaccine disclosed by the invention canbe directly fed by being added into feed to avoid or reduce the outbreak of the spring viraemia disease of carps, and has a relatively good immunoprophylaxis effect on the spring viraemia of the carps.

Description

technical field [0001] The invention relates to the technical field of immune prevention and treatment of carp diseases, in particular to an oral vaccine for carp spring viremia that can be added to feed. Background technique [0002] Carp, commonly known as carp guaizi, Maozi, etc., belongs to Cyprinidae and is one of the main freshwater fish species in my country, accounting for more than half of the total freshwater fish in my country. The side of the body is flat and the abdomen is round, the mouth is horseshoe-shaped, and there are 2 pairs of whiskers. The base of the dorsal fin is longer, and both the dorsal and anal fins have a thick and serrated spine. Body side golden yellow, caudal fin lower lobe orange red. Carp usually inhabit the bottom of water bodies overgrown with aquatic plants in rivers, lakes, reservoirs, and ponds, and mainly feed on benthic animals. Because carp is rich in nutrients and has the effect of lowering cholesterol, preventing arterioscleros...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/75C12N15/47A61K39/205A61P31/14A23K10/18A23K50/80A01K61/13C12R1/125
CPCA61K39/12A61K2039/523A61K2039/552A23K10/18A23K50/80A01K61/13A61P31/14C07K14/005C12N15/75C12N2760/20022C12N2760/20034Y02A40/81
Inventor 林锋陈建明黄爱霞郭建林孙丽慧姜建湖
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
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