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Novel method for detecting metabolite spatial distribution in fresh tea leaves based on targeted metabonomics

A technology of tea tree fresh leaves and metabolomics, which is applied in the field of tea compound detection, can solve the problems of lack of spatial distribution of metabolites in tea tree fresh leaves, small number of metabolites, difficulty in controlling high sensitivity, etc., and achieve low performance requirements and wide range Application prospect, low cost effect

Active Publication Date: 2020-01-31
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently a lack of detection methods for the spatial distribution of metabolites in fresh leaves of tea trees, and there are very few studies on this aspect.
The currently applied detection method for the spatial distribution of metabolites in fresh leaves of tea trees still has some shortcomings, such as: only a small amount of high-concentration metabolites can be detected by mass spectrometry imaging technology, expensive mass spectrometers are required, and the ionization of the compounds is easily affected by fresh leaves. The impact of the waxy layer on the surface; by detecting the metabolites of millimeter-scale fragments to restore the spatial distribution in the tea tree fresh leaf tissue, it is difficult to control the required high sensitivity; the desorption electrospray ionization imaging mass spectrometry method can detect The number of metabolites is very small

Method used

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  • Novel method for detecting metabolite spatial distribution in fresh tea leaves based on targeted metabonomics
  • Novel method for detecting metabolite spatial distribution in fresh tea leaves based on targeted metabonomics
  • Novel method for detecting metabolite spatial distribution in fresh tea leaves based on targeted metabonomics

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Embodiment 1

[0029] (1) Micro-scale sample pretreatment of fresh leaves of tea trees: fresh leaves of tea trees of Longjing 43 varieties were collected, and the position was the first leaf. Use a scalpel and tweezers to cut the above fresh tea tree leaves into small square pieces with a side length of 1.7 mm × 1.7 mm, and record and number the position of each square small piece in the fresh tea tree leaves. This operation is done on ice .

[0030] (2) Extraction of metabolites in the fresh leaves of tea trees: the fresh leaves of tea trees after the pretreatment described in step (1) are placed in a centrifuge tube, with 100 microliters of 50% acetonitrile aqueous solution as a solvent, the extraction temperature is 20 ° C, and the extraction time is Take 30 minutes to extract the metabolites in the fresh leaves of the tea tree; after the extraction is completed, centrifuge at a high speed at a speed of 10,000 rpm for 10 minutes to obtain the supernatant, which is the extract.

[0031] (...

Embodiment 2

[0039](1) Micro-scale sample pretreatment of fresh leaves of tea trees: collect fresh leaves of Longjing 43 varieties of tea trees, the position is the first leaf, and two places on the veins are used for acupuncture treatment, and the acupuncture positions are figure 2 Circle, then leave for 30min. Use a scalpel and tweezers to cut the above fresh tea tree leaves into small square pieces with a side length of 1.7 mm × 1.7 mm, and record and number the position of each square small piece in the fresh tea tree leaves. This operation is done on ice .

[0040] (2) Extraction of metabolites in the fresh leaves of tea trees: the fresh leaves of tea trees after the pretreatment described in step (1) are placed in a centrifuge tube, with 100 microliters of 50% acetonitrile aqueous solution as a solvent, the extraction temperature is 20 ° C, and the extraction time is Take 30 minutes to extract the metabolites in the fresh leaves of the tea tree; after the extraction is completed, c...

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Abstract

The invention belongs to the technical field of tea compound detection, and particularly relates to a novel method for detecting metabolite spatial distribution in fresh tea leaves based on targeted metabonomics. The method comprises the following steps: (1) pretreating micro-scale samples of the fresh tea leaves; (2) extracting metabolites in the fresh tea leaves; (3) performing chemical derivatization on an extracting solution; (4) detecting the metabolites based on the targeted metabonomics; and (5) reducing and visually displaying the metabolite spatial distribution in the fresh tea leaves. The method is accurate, reliable, simple and feasible, can simultaneously analyze the spatial distribution of nearly 50 metabolites in the fresh tea leaves, and has important significance in synthesis and transportation of the metabolites in tea trees, research of the tea trees on biological and abiotic stress and the like.

Description

technical field [0001] The invention belongs to the technical field of tea compound detection, and more specifically relates to a new method for detecting the spatial distribution of metabolites in fresh leaves of tea trees based on targeted metabolomics. Background technique [0002] Tea is one of the most popular beverages in the world and a natural health supplement. According to statistics, tea contains more than 450 metabolites that are beneficial to the human body. Understanding the spatial distribution of metabolites in fresh tea leaves is of great significance for studying how metabolites are biosynthesized and transported in fresh tea leaves, and how tea leaves respond to external stimuli and enhance tea quality. However, there is currently a lack of detection methods for the spatial distribution of metabolites in fresh leaves of tea trees, and there are very few studies on this aspect. The currently applied detection method for the spatial distribution of metabol...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72G01N30/06G01N30/34
CPCG01N30/02G01N30/72G01N30/06G01N30/34G01N2030/067
Inventor 戴伟东林智彭群华谭俊峰朱荫施江郭丽张悦
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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