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Active freeze-dried powder capable of controlling diseases of loquats after picking as well as preparation method and application of active freeze-dried powder

A postharvest disease, freeze-dried powder technology, applied in microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problem of low survival rate of bacteria, reduce rot index, improve survival rate, soluble protein and the effect of reducing the Vc content

Active Publication Date: 2020-01-31
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that ice crystals can easily cause mechanical damage to cells, cause protein denaturation and inactivation in cells, and reduce the number of viable bacteria. Therefore, it is necessary to add protective agents to reduce damage. The types and concentrations of protective agents for different bacterial species There have been many research results on the screening of the ratio, but there is still the problem of low survival rate of the bacteria in the freeze-dried product, but there are few reports on the freeze-dried product of loquat bio-control yeast

Method used

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  • Active freeze-dried powder capable of controlling diseases of loquats after picking as well as preparation method and application of active freeze-dried powder
  • Active freeze-dried powder capable of controlling diseases of loquats after picking as well as preparation method and application of active freeze-dried powder
  • Active freeze-dried powder capable of controlling diseases of loquats after picking as well as preparation method and application of active freeze-dried powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: the preparation of the active freeze-dried powder of preventing and treating loquat postharvest disease

[0041] Activation of the strain: pick the Metschnikowia pulcherrima E1 preserved on the slant and inoculate it in the NYDA medium by streaking, and culture at a constant temperature of 28°C for 2-3 days; the composition of the NYDA medium is: beef extract 8g, yeast extract Paste 5g, glucose 10g, agar powder 20g, supplemented with distilled water to 1000mL, natural pH, sterilized at 121°C for 20min.

[0042] The morphological characteristics of Metschnikowia pulcherrima E1 are as follows: figure 1 As shown, after being cultured on NYDA medium at 28°C for 2 days, the colony is round, small, opaque, cream-colored, with neat edges, moist and smooth surface, easy to stir up, producing red pigment, and the opposite side is reddish brown. Microscopically, the cells are spherical or nearly spherical, with unilateral or multilateral budding.

[0043] The stra...

Embodiment 2

[0051] Embodiment 2: The component optimization of each protective agent in the active freeze-dried powder of preventing and treating loquat postharvest disease

[0052] In this embodiment, the composition and content of the freeze-dried powder protective agent are optimized, and the specific method is as follows:

[0053] 1. Single factor test

[0054] By consulting the literature, 8 kinds of protective agents were selected to prepare protective agent solutions with different mass concentrations, and then the yeast sludge was resuspended to make a bacterial suspension for freeze-drying. The samples before freeze-drying were diluted and counted, and the samples after freeze-drying After the sample was rehydrated and activated by adding an equal volume of normal saline, it was also diluted and counted, and the freeze-dried survival rate of the strain was calculated (survival rate=number of viable bacteria before freeze-drying / number of viable bacteria before freeze-drying×100%)...

Embodiment 4

[0078] Embodiment 4: the control effect of the active freeze-dried powder of preventing and treating loquat postharvest disease on loquat gray spot disease

[0079] Resuspend the lyophilized powder with sterile water and adjust the concentration to 1×10 8cells / mL, for use; Inoculate PDA medium with the pathogenic bacterium Pestalotiopsis vismiae P2 (Pestalotiopsis vismiae P2) of loquat gray spot disease, culture it at 28°C for 7-10 days, and prepare it with sterile water to a concentration of 1×10 5 spores / mL conidia suspension, set aside. Among them, Pestalotiopsis vismiae P2 was screened by our laboratory and was deposited in the China Center for Type Culture Collection (CCTCC) on April 15, 2019. The deposit address is: Wuhan University, China, and the deposit number is CCTCC NO:M 2019257; the proposed taxonomic name is Pestalotiopsis vismiae P2.

[0080] Choose loquat fruits with uniform size and no mechanical damage, rinse them with clean water first, then soak them in 0...

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Abstract

The invention relates to active freeze-dried powder capable of controlling diseases of loquats after picking as well as a preparation method and application of the active freeze-dried powder, and belongs to the technical field of biocontrol of fruits after picking. The freeze-dried powder comprises Metschnikowia pulcherrima E1, lactose, sodium glutamate, sorbitol and inulin. NYDB is inoculated with activated Metschnikowia pulcherrima E1 for enrichment culture, then, a culture solution is centrifuged to collect bacterial sludge, the bacterial sludge is suspended again by a protective agent solution to produce a bacterial suspension, and vacuum freeze-drying is performed after packaging to obtain freeze-dried powder. During use, sterile water is used for suspension, the yeast concentration is adjusted to 1*10<8> cells / mL, loquats are soaked in the bacterial liquid for 5 min and then taken out or the bacterial liquid is directly and uniformly sprayed on surfaces of loquat fruits and airing is performed. Active freeze-dried powder treatment can significantly reduce incidence and grey speck disease of loquat fruits and diameter of specks, significantly reduce the rotting index of loquatfruits, inhibit increase of fruit weight loss ratio and peel cell membrane permeability, and delay reduction of content of soluble solids, titratable acid, soluble protein and Vc of loquat fruits.

Description

technical field [0001] The invention relates to an active freeze-dried powder for preventing and treating postharvest diseases of loquats, a preparation method and application thereof, and belongs to the technical field of postharvest biological control of fruits. Background technique [0002] Loquat (Eriobotrya japonica. IAndl) belongs to Rosaceae (Rosaceae) and Loquat genus (Eriobotrya). It is a unique fruit in the subtropical zone of my country. Consumer favorite. The loquat peel is thin, and it is extremely vulnerable to mechanical damage and rot during harvesting and transportation. Studies have shown that postharvest loquat rot is mainly caused by infectious diseases such as anthracnose and gray spot. Among them, loquat gray spot disease can harm the branches, leaves, flowers and fruits of loquat, and the loquat fruit can be damaged from young fruit to mature stage. [0003] For a long time, the control of loquat postharvest diseases has mainly relied on physical and...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/04A23B7/155A23B7/154C12R1/645
CPCC12N1/04A23B7/155A23B7/154A23V2002/00C12R2001/645C12N1/145A23V2200/10A23V2250/76A23V2250/612A23V2250/642A23V2250/5062
Inventor 孙卫红杨慧慧王玲赵一繁孙沣震李胜杰
Owner JIANGSU UNIV
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