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Aspergillus niger genetic engineering bacterial strain high in yield of malic acid under condition of low pH and application

A technology of genetically engineered strains and Aspergillus niger, applied in the field of genetic engineering, can solve problems such as undiscovered patent publications, and achieve the effects of increasing process difficulty, purification cost, and production cost

Active Publication Date: 2020-01-31
南京昊禾生物科技有限公司
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Problems solved by technology

[0007] Through the search, no patent publications related to the patent application of the present invention have been found

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  • Aspergillus niger genetic engineering bacterial strain high in yield of malic acid under condition of low pH and application
  • Aspergillus niger genetic engineering bacterial strain high in yield of malic acid under condition of low pH and application
  • Aspergillus niger genetic engineering bacterial strain high in yield of malic acid under condition of low pH and application

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Embodiment Construction

[0030] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0031] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0032] A high-yield malic acid Aspergillus niger genetically engineered strain under low pH conditions, the construction steps of the genetically engineered strain are as follows:

[0033]Step 1: Knock out the Peroxins protein gene pexG responsible for localization of isocitrate lyase on peroxisomes, which is responsible for transporting newly synthesized isocitrate lyase to the peroxisome matrix; first, black Aspergillus niger (Aspergillus niger) genome is used as a template, and the sequence fragments of the upstrea...

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Abstract

The invention relates to an aspergillus niger genetic engineering bacterial strain high in yield of malic acid under condition of low pH. The aspergillus niger genetic engineering bacterial strain isprepared through the steps of obtaining a pexG gene knock-out bacterial strain S914; obtaining an expression Malate synthase gene mas* bacterial strain S1016; overexpressing aspergillus niger malic acid transport protein gene dct1, performing transformant screening and hygromycin resistant gene reorganization to obtain an overexpressing dct1 bacterial strain S1140 without a screening mark, namelythe aspergillus niger genetic engineering bacterial strain high in yield of malic acid under condition of low pH. The bacterial strain is based on the natural characteristics that the aspergillus niger resists acid and generates organic acid, through heredity reorganization, the physiological property of the aspergillus niger is reformed, and experiments confirm that the capacity of the aspergillus niger genetic engineering bacterial strain for generating malic acid at low pH can be notably improved. A good strain is provided for preparation of apples by a microbial fermentation method.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to a genetically engineered strain of Aspergillus niger with high malic acid production under low pH conditions and its application. Background technique [0002] Malic acid is widely used in food, health care, medicine, daily chemical and chemical industry. Because L-malic acid has a soft taste and high acidity, does not damage the oral cavity and teeth, and does not accumulate fat, it has become a low-calorie food sour agent recognized by the international food industry as a safe food. One of the good organic acids. In the pharmaceutical industry, L-malic acid is used to treat various diseases such as liver disease, anemia, and uremia. And because L-malic acid is beneficial to the absorption of amino acids in metabolism, it is often formulated into compound amino acid injections. Therefore, the demand for L-malic acid is increasing day by day in the international ma...

Claims

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Application Information

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IPC IPC(8): C12N1/15C12P7/46C12R1/685
CPCC12P7/46C12N9/1025C12Y203/03009C07K14/38
Inventor 刘浩曹威单璐黄和徐晴
Owner 南京昊禾生物科技有限公司
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