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A Fluorescent Dye Detection Method Based on Noble Metal/Semiconductor Controlled Luminescent Thin Film Substrate

A luminescent film and fluorescent dye technology, applied in the field of fluorescent dye detection, can solve problems such as unsatisfactory dye detection methods, inability to use base films in interoperability, and inability to qualitatively analyze dyes, achieving reproducibility, safety, non-toxicity, and improved detection. Sensitivity, the effect of enhancing the intensity of the Raman spectrum

Active Publication Date: 2022-07-22
DALIAN NATIONALITIES UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

However, this common detection method has always had the defect of linear saturation, that is, the quantitative analysis of intensity and concentration can only be realized in a certain small range at low concentrations.
In addition, since fluorescence resonance energy transfer can occur between luminescent nanoparticles and fluorescent dyes, the use of luminescent thin films to detect dyes has gradually attracted the attention of scientists. qualitative analysis
Since the detection methods have their own characteristics, and the base film cannot be used interchangeably, the dye detection method has not been ideal

Method used

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  • A Fluorescent Dye Detection Method Based on Noble Metal/Semiconductor Controlled Luminescent Thin Film Substrate
  • A Fluorescent Dye Detection Method Based on Noble Metal/Semiconductor Controlled Luminescent Thin Film Substrate
  • A Fluorescent Dye Detection Method Based on Noble Metal/Semiconductor Controlled Luminescent Thin Film Substrate

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Embodiment 1

[0028] The invention provides a novel fluorescent dye detection method based on a noble metal / semiconductor regulated luminescent film substrate. The semiconductor W in the composite film is 18 O 49 The nanowire film is a substrate with thorn-like morphology grown on conductive glass. Taking advantage of its uneven surface, the noble metal Au nanorods are deposited to improve the local electromagnetic field on the film surface, and the secondary deposition of luminescent nanoparticles enhances the luminous intensity. After the dye is spin-coated on the surface of the substrate, the contact of the exposed noble metal / semiconductor is used to increase the Raman scattering signal of the dye at a low concentration, and the Raman characteristic peak of the dye is obtained to qualitatively analyze the dye composition. Then in noble metal / semiconductor tuning enhanced NaYF 4 : Yb,Er luminescent film was tested for the fluorescence spectra of different concentrations of dyes, and the...

Embodiment 2

[0039] The invention provides a novel fluorescent dye detection method based on a noble metal / semiconductor regulated luminescent film substrate. The semiconductor W in the composite film is 18 O 49 The nanowire film is a substrate with thorn-like morphology grown on the conductive glass. Taking advantage of its uneven surface, the noble metal Au nanorods are deposited to improve the local electromagnetic field on the film surface, and the secondary deposition of luminescent nanoparticles enhances the luminous intensity. After the dye is spin-coated on the surface of the substrate, the contact of the exposed noble metal / semiconductor is used to increase the Raman scattering signal of the dye at a low concentration, and the Raman characteristic peak of the dye is obtained to qualitatively analyze the dye composition. Then in noble metal / semiconductor tuning enhanced NaYF 4 : Yb,Er luminescent film was tested for the fluorescence spectra of different concentrations of dyes, and...

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Abstract

The invention belongs to the field of fluorescent dye detection, and discloses a fluorescent dye detection method based on a noble metal / semiconductor regulated luminescent film substrate. The method comprises the following steps: S1. preparation of a noble metal / semiconductor regulated light-emitting thin film substrate; S2. uniformly spin-coating dye probes on the substrate to obtain a standard sample; S3. measuring the Raman spectrum of the obtained standard sample—qualitatively; S4. Draw and analyze the standard curve of Raman intensity and dye concentration; S5. Test the fluorescence spectrum of the obtained standard sample; S6. Draw and analyze the standard curve of spectral integral area and dye concentration - quantification. The invention adopts the double detection method, overcomes the defect of linear saturation of Raman detection, and realizes accurate detection from qualitative to quantitative by using the "fingerprint" characteristics and fluorescence sensing characteristics of Raman detection. The detection method of the invention is novel and unique, has low cost, is simple and practical, and has strong anti-interference ability.

Description

technical field [0001] The invention belongs to the field of fluorescent dye detection, and relates to a fluorescent dye detection method based on a noble metal / semiconductor regulated luminescent film substrate. Background technique [0002] In recent years, the detection of low-concentration and small-volume dyes has great significance in the fields of medicine, materials and environmental protection. Surface-enhanced Raman scattering is commonly used to detect dyes due to its high sensitivity and simple operation, which can provide "fingerprint" information. It is widely used in environmental monitoring, detection of agriculture, chemical biology, and food safety. In Raman detection, a substrate with strong plasmon resonance properties is used, and the dye composition is determined by the characteristic Raman scattered light of the dye under laser irradiation. However, this common detection method has always had the defect of linear saturation, that is, quantitative anal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/65C09K11/85
CPCG01N21/64G01N21/6428G01N21/643G01N21/65G01N21/658C09K11/7773G01N2021/6417G01N2021/6439G01N2021/655
Inventor 丛妍杨扬董斌尚景雨
Owner DALIAN NATIONALITIES UNIVERSITY
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