DNA hybridization detection method based on surface enhanced Raman scattering spectrum technology

Abstract

The invention discloses a DNA hybridization detection method based on a surface enhanced Raman scattering spectrum technology. The DNA hybridization detection method comprises the following steps: (1)preparing a silver nanoparticle turbid liquid with the diameter of 30 nanometers, (2) adding a sulfhydrylated DNA probe into the turbid liquid prepared in the step (1), (3) adding a DNA sample to bedetected into the silver nanoparticle turbid liquid riveted with the DNA probe in the step (2), and (4) adding a magnesium sulfate solution, negatively-charged silver nanoparticles and a DNA probe into the turbid liquid obtained in the step (3), clustering under the bridging action of divalent magnesium ions to form a sandwich cluster structure, and finally, fixing a centrifugal tube filled with the sandwich cluster structure on a glass tube support for Raman spectrum measurement. According to the method, nondestructive testing of the DNA test article in the DNA measurement process is guaranteed, reliable distinguishing of DNA hybridization signals is achieved, the measurement precision is high, the speed is high, and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of optical devices for DNA detection, and in particular relates to a DNA hybridization detection method based on surface-enhanced Raman scattering spectroscopy technology. Background technique [0002] The detection of DNA and proteins is crucial for the diagnosis and treatment of genetic diseases, as well as the detection of infectious agents, drug discovery, etc. Such biological detection usually relies on DNA hybridization or antigen-antibody interaction. Ultrasensitive detection technology realizes DNA hybridization or Direct and efficient detection of antigen-antibody interaction is of great significance. Electrochemical sensors have received extensive attention and research by scholars due to their ultra-high sensitivity, inherent simplicity and miniaturization, low cost and low power consumption. The main principle of the electrochemical sensor detection method is to first rivete a DNA recognition pr...

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Problems solved by technology

Another approach to DNA hybrid sensing is to take advantage of the ability of double-stranded DNA itself to act as a conduit for electron transfer over long distances with a small decrease in electron transfer rate with distance, while the electron transfer efficiency of single-stranded DNA is very low.

The use of electrochemical sensing to detect DNA hybridization has been researched and developed a lot, but due to the complexity and low controllability of the electrochemical reaction itself, especially the selection of electrode materials and redox indicators, the development of electrochemical sensing technology further development is hindered

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