Kit for detecting hot spot mutation of human TERT gene promoter
A TERT-124 and promoter technology, applied in the biological field, can solve the problems of complex operation, high detection cost, expensive detection equipment, etc., and achieve the effects of high detection sensitivity, high universality, and clear and objective interpretation of results.
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[0023] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.
[0024] The self-prepared instruments required by the kit of the present invention are as follows: various fluorescent quantitative PCR instruments, various conventional PCR thermal cyclers, ultraviolet spectrophotometers, high-speed centrifuges, vortex oscillators, and sterilized water for injection.
[0025] Self-prepared reagents required for the kit of the present invention: DNA extraction kit, common DNA product purification reagent.
[0026] 1. DNA enrichment routine PCR reaction
[0027] (1) Mixed reagents for PCR reaction: various substances at the following concentrations are added to the premixed reagents:
[0028] name Dosage (final concentration) Mastermix 12.5μl(-) 163-F (SEQ ID NO.1) primer 2μl (0.4μM) 163-R (SEQ ID NO.2) primer 2μl (0.4μM) Sterile water for injection 6.5μl(-) DNA template 2μl...
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