Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of TaPT13 gene in improving resistance of plants to Gaeumannomyces graminis var. tritici J. Walker

A gene and plant technology, applied in the application field of gene TaPT13 in improving plant resistance to graminosa wheat variety, to achieve the effects of improving plant disease resistance, improving variety resistance, and promoting efficient absorption

Active Publication Date: 2020-01-21
ZHOUKOU NORMAL UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Previous research on phosphorus transporters has mainly focused on the role of plants in the uptake and redistribution of inorganic phosphate, and the role of phosphorus transporters in plant defense has rarely been reported.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of TaPT13 gene in improving resistance of plants to Gaeumannomyces graminis var. tritici J. Walker
  • Application of TaPT13 gene in improving resistance of plants to Gaeumannomyces graminis var. tritici J. Walker
  • Application of TaPT13 gene in improving resistance of plants to Gaeumannomyces graminis var. tritici J. Walker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1.1 Mycorrhizal and low phosphorus treatment

[0025] The wheat at the three-leaf stage was transplanted into the medium of fine sand, vermiculite and perlite mixed with G. The amount is 200 spores / 15g. Grow in a light incubator with a photoperiod of 16h / 8h and a temperature of 18°C / 15°C. All inoculated and control plants were watered twice a week with 1 / 2 Hoagland nutrient solution (5 μM KH 2 PO 4 Low phosphorus treatment, 500μM KH 2 PO 4 High phosphorus treatment), 6 weeks later, the wheat roots were taken, washed with clean water, and the root samples were harvested, frozen in liquid nitrogen and stored at -80°C for later use.

[0026] Total RNA was extracted from the mycorrhizae and the samples treated with low phosphorus and high phosphorus using TRIzol Reagent (invitrogen).

[0027] Using 1 μg of total RNA as a template, the first-strand cDNA was synthesized using a cDNA synthesis kit, and the extracted RNA was reverse-transcribed into cDNA.

[0028] Real-ti...

Embodiment 2

[0034] Functional analysis of the TaPT13-VIGS plant mediated by embodiment 2TRV virus

[0035] Using the full length of the TaPT13 gene as a template, according to the carrier characteristics of the TRV viral vector pYL156, EcoR I and BamH I sites were selected to construct pYL156, and the recombinant vector pYL156-TaPT13 and empty pYL156 were transformed into Agrobacterium GV3101 (negative control) , the use of newly germinated wheat seeds to extract and transform the TRV vector to achieve silencing of the whole wheat plant.

[0036] The inoculation method of the wheat var. graminosa adopts the bacterial block inoculation method, and gets the fresh bacterial block (5 * 5cm) that cultivates 7d on the PDA medium. 2 ) were inoculated on the roots of TaPT13-VIGS and control plant seedlings, and after cultivating in the light incubator for 21 days (day 20°C / night 12°C, light cycle 16h / 8h, relative humidity 50%), the diseased part of the stem base was firstly placed Fix in destain...

Embodiment 3

[0048] Example 3 Functional Analysis of Overexpression TaPT13 in Arabidopsis

[0049] In order to further analyze the function of TaPT13, the full length of TaPT13 was cloned and constructed into the plant vector CTAPi-GW-3HA promoted by the 35S promoter, and the overexpression recombinant vector CTAPi-GW-3HA-TaPT13 was obtained, and the recombinant vector and the empty vector were passed through The heat shock method was used to transform Agrobacterium GV3101, and the recombinant vector and the empty vector were transformed into Arabidopsis thaliana of the Columbia ecotype (Col-0) by dipping flowers. After 3 generations of screening, 5 single-copy homozygous transgenic lines were obtained. The roots of wild-type Arabidopsis and transgenic plants were simultaneously inoculated with T. graminearum (preliminary experiments found that wild-type Arabidopsis is the host of G. graminearum).

[0050] The results showed that compared with the wild-type and empty vector transgenic plan...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of agricultural biology, and particularly relates to application of a TaPT13 gene in improving the resistance of plants to Gaeumannomyces graminis var. tritici J. Walker. According to the invention, the arbuscular mycorrhiza-specific phosphorus transport protein gene TaPT13 can be used for improving plant disease resistance, with the nucleotide sequence shown asSEQ ID NO. 1. The invention determines that the TaPT13 gene is an arbuscular mycorrhiza-specific phosphorus transport protein gene, and the TaPT13 gene expression level is significantly increased after the plant is inoculated with the Gaeumannomyces graminis var. tritici J. Walker; and the susceptibility of the plant is increased, and the expression levels of disease-resistant marker genes are decreased after silencing the TaPT13 gene in the plant. Therefore, the TaPT13 gene can be used for improving the resistance of wheat to the Gaeumannomyces graminis var. tritici J. Walker, and has wide application space in plant breeding and cultivation.

Description

technical field [0001] The invention belongs to the field of agricultural biology, and in particular relates to the application of gene TaPT13 in improving the resistance of plants to wheat varietal. Background technique [0002] Phosphorus is the second largest element necessary for plant growth and development. It is widely involved in energy transfer, signal transduction, biosynthesis of macromolecules, photosynthesis, regulation of respiration and other metabolic processes, and plays an important role in plant growth. Phosphorus exists mainly in the form of complexes, insolubles and organic forms in soil, while plants mainly obtain phosphorus in the form of inorganic phosphorus, so phosphorus in soil is difficult to be absorbed and utilized by plants. Plants have evolved a series of effective strategies to promote phosphorus uptake and utilization in response to the stress of low phosphorus environment, including changing root system structure, root system secretion of o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00A01H6/46
CPCC07K14/415C12N15/8282C12N15/8243Y02A40/146
Inventor 张怡李成伟李俐俐李晓丽张菊徐克东于德水雷杰
Owner ZHOUKOU NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com