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Lysinibacillus S12 and application thereof

A technology of Bacillus lysine, S12, applied in the application, bacteria, plant cultivation and other directions, can solve the problems of low yield and chemical pollution, and achieve the effect of increasing yield, reducing dosage, and improving production economic and social benefits

Active Publication Date: 2020-01-21
青岛润博特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of the deficiencies in the prior art, the technical problem to be solved by the present invention is to provide a bacterium that promotes the growth of Agaricus bisporus; another technical problem to be solved by the present invention is to provide the application of lysine bacillus S12 in promoting the growth of Agaricus bisporus, to Solve the problems of low yield and high chemical pollution in traditional Agaricus bisporus planting methods

Method used

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  • Lysinibacillus S12 and application thereof
  • Lysinibacillus S12 and application thereof
  • Lysinibacillus S12 and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] 1) Isolation, screening and identification of bacteria

[0024] A total of 78 bacterial isolates were isolated from the rhizosphere soil samples by gradient dilution method from the planting base of Edible Fungi Science and Technology Industrial Park in Zhuyang Town, Tai'an, Shandong Province, and selected to enriched agar Culture medium (5g of beef extract, 10g of peptone, 5g of sodium chloride, 20g of agar, pH 7.2-7.4) was cultured for 5-7 days, and the bacterial single colony isolates that formed a transparent circle around them were selected.

[0025] Put the strains preserved after primary screening, separation and purification into conical flasks of NA medium, and culture them on a shaker at 28°C and 180r / min for 48 hours to obtain a cultured saturated bacterial liquid, and use a pipette according to the aseptic operation requirements Inoculate 0.2mL of each bacterial solution on a PDA plate (glucose 20g, potato 200g, peptone 10g, yeast extract 3g, agar 15-20g, wa...

Embodiment 2

[0033] Under natural conditions, the soil is mixed with organic matter to effectively carry out anaerobic fermentation. Take a small piece of land in the field after wheat or rapeseed or near the water source to cultivate Agaricus bisporus. Take the land and dig 25-30cm deep. Crush the soil until it is as fine as possible without mud, and then add crushed dry cow dung, wheat husk or chaff, lime, and superphosphate. Mix these substances with the soil well, and then water them to keep them warm. After pounding the soil and fermenting evenly, take the soil, and then use physical and chemical methods to disinfect the soil. After the disinfection, put it into the mushroom bed, and put the prepared culture material in the mushroom bed. The thickness of the covering soil should depend on the thickness of the compost. The thickness of the compost for high-yield cultivation is 18-22cm, and the thickness of the covering soil should be 3.5-4.0cm. After the covering soil is made, dilute ...

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Abstract

The invention discloses a lysinibacillus S12 and application thereof, and relates to the biotechnology field of edible fungi. The classification and the naming of the lysinibacillus S12 is (Lysinibacillus sp.) S12, the lysinibacillus S12 is collected in the China Center for Type Culture Collection, wherein a collection number is CCTCC NO: M 2019713, a collection date is 11th, September, 2019, anda collection address is Wuhan University, Wuhan, China. The lysinibacillus S12 disclosed by the invention is applied to agaricus bisporus growth, agaricus bisporus hyphae can be effectively accelerated to grow, the production period of the agaricus bisporus is shortened, the yield of the agaricus bisporus is improved, a product is guaranteed to be environmentally-friendly, healthy and pollution-free and is non-toxic and harmless, and the economic and social benefit of edible fungi production can be improved.

Description

technical field [0001] The invention relates to the field of edible fungus biotechnology, in particular to a strain of Bacillus lysinus S12 and its application. Background technique [0002] Agaricus bisporus is one of the most common edible fungi, with thick meat. The mycelium is silvery white, the growth rate is relatively fast, and it is not easy to form a vegetative indument. The fruiting body is mostly solitary, round and white, without scales, the cap is thick, and it is difficult to open the umbrella. The tissue is firm, with a semi-membrane ring on the stipe, and the spores are silvery brown. Agaricus bisporus is an edible fungus that is widely cultivated, high in production and high in consumption. It is rich in protein, sugar, various nucleotides, vitamins and unsaturated fatty acids. It is rich in nutrition, delicious in taste and high in edible value. It has the effects of anti-tumor, enhancing immunity, lowering blood pressure, etc., and has obvious medicinal ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01G18/20C12R1/01
CPCC12N1/20A01G18/20C12R2001/01C12N1/205
Inventor 李冠喜于颖媛张璐焦玉茹李秋月亓超凡张雯倩郭帅
Owner 青岛润博特生物科技有限公司
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