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Miao medicine Periploca forrestii Schltr. HPLC fingerprint study and multi-component content determination method

A technology of fingerprints and determination methods, which is applied in the field of HPLC fingerprint research and multi-component content determination of the Miao medicine Heiguteng, which can solve problems such as differences in the content of the components of Heiguteng

Active Publication Date: 2020-01-17
贵州中医药大学
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Problems solved by technology

[0003] However, there is a big difference in the content of each component of Teng Teng from different origins. In order to provide a basis for the quality evaluation of Teng Teng medicinal material, a method for HPLC fingerprinting and determination of multi-component content of Miao medicine Teng Teng was developed.

Method used

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  • Miao medicine Periploca forrestii Schltr. HPLC fingerprint study and multi-component content determination method
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  • Miao medicine Periploca forrestii Schltr. HPLC fingerprint study and multi-component content determination method

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Embodiment Construction

[0093] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, but not as a basis for limiting the present invention.

[0094] Example. A kind of Miao medicine Heiguteng HPLC fingerprint chromatogram research and multi-component content determination method, the composition is as follows Figure 1-3 shown, including the following steps:

[0095] a. Preparation of the test solution: Pulverize the syringa vine through a 24-mesh sieve, store the powder in a desiccator, accurately weigh 1.000g of the syringa powder, put it in a 50mL conical flask with a stopper, add 20mL of 50% methanol , weigh the weight, use power 200W, frequency 40KHz to carry out ultrasonic extraction for 30min, after cooling to room temperature, supplement the weight lost in the ultrasonic process with 50% methanol, shake well, pass through a 0.22μm microporous membrane, and get the subsequent filtrate, namely have to;

[0096] b. Preparation of ...

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Abstract

The invention discloses a Miao medicine Periploca forrestii Schltr. HPLC fingerprint study and multi-component content determination method. An HPLC method is used to establish fingerprints of Periploca forrestii Schltr. of different producing areas, contents of four kinds of caffeoyl quinic acid-type chemical constituents therein are determined, and a basis is provided for Periploca forrestii Schltr. medicinal material quality evaluation; a chromatographic column is Xtimate C18 (4.6mm x 250mm, 5[mu]m); 0.1% phosphoric acid aqueous solution (A)-acetonitrile (B) is used as mobile phases, and gradient elution is carried out; a flow rate is 1 mL / min; a column temperature is 30 DEG C; injection volume is 10[mu]L, and detection wavelength is 330nm; and a similarity evaluation system for chromatographic fingerprint of TCM (version 2012) is used for similarity analysis on samples, contents of four kinds of caffeoyl quinic acid-type chemical constituents can be determined simultaneously through the Periploca forrestii Schltr. HPLC fingerprints and content determination, the four kinds of constituents of the different producing areas have large differences, the content of each constituent has a certain relationship with a producing area, and a foundation is laid for later Miao medicine Periploca forrestii Schltr. quality standard study.

Description

technical field [0001] The invention relates to a fingerprint research and multi-component content determination method, in particular to an HPLC fingerprint research and multi-component content determination method of the seedling medicine Heliconia japonica. Background technique [0002] Black bone vine is the dry root or whole plant of Periploca Linn. (Periploca forrestii Schltr.) in the family Asclepiadaceae. Properties: pungent, bitter, hot in nature; slightly poisonous. Enter the Leng Jing. Expelling wind and removing dampness, promoting blood circulation and eliminating carbuncle. Cure mainly bruises, rheumatic arthralgia, amenorrhea, mastitis, fractures, etc. It is a folk medicine widely used in the treatment of closed soft tissue injuries, rheumatism and rheumatoid diseases. The chemical components include: cardiac glycosides, flavonoids, caffeoylquinic acids, phenylpropanoids, triterpenoids, steroids, etc. [0003] However, there are large differences in the c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8686
Inventor 刘刚刘育辰唐萍代玉洁
Owner 贵州中医药大学
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