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A kind of carbon quantum dot fluorescent probe, test paper and its application in the detection of morin

A technology of carbon quantum dots and fluorescent probes, applied to test paper and its application in the detection of morin, in the field of carbon quantum dots fluorescent probes, which can solve the problems of low detection sensitivity and long time consumption.

Active Publication Date: 2021-08-13
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing detection methods such as liquid chromatography and gas chromatography are time-consuming and have low detection sensitivity

Method used

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  • A kind of carbon quantum dot fluorescent probe, test paper and its application in the detection of morin
  • A kind of carbon quantum dot fluorescent probe, test paper and its application in the detection of morin
  • A kind of carbon quantum dot fluorescent probe, test paper and its application in the detection of morin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Preparation of polyethyleneimine carbon quantum dots: First, weigh 100 mg of PEI, dissolve it in 10 mL of twice-distilled water, and prepare a 10 mg / mL PEI solution; take 10 mL of the prepared solution, and transfer the reaction system to a polytetrafluoroethylene In an ethylene hydrothermal kettle, the reaction temperature is 160°C, and the reaction time is 6 hours; wrap the centrifuge tube with tin foil to control the internal environment as a light-proof environment; after the reaction is completed, transfer the reaction system to a centrifuge tube with tin foil, Store in a refrigerator at 4°C, and the reaction system does not need further purification. The concentration of the carbon quantum dot fluorescent probe solution was measured with the concentration of PEI.

Embodiment 2

[0026] Preparation of HEPES buffer solution: Weigh 4.766 g of HEPES and add 1 L of distilled water to prepare a 20 mM HEPES buffer solution with pH=7.4.

Embodiment 3

[0028] Utilize the carbon quantum dot fluorescent probe to detect morin in the HEPES buffer solution of pH=7.4: take 10 μ L of the carbon quantum dot fluorescent probe solution prepared in Example 1, add 990 μ L HEPES buffer solution of pH=7.4, promptly be prepared into 0.1 mg / mL carbon quantum dot fluorescent probe solution, in each 1mL diluted carbon quantum dot fluorescent probe solution, add the mother liquor of morin respectively, so that the final concentrations of morin in the system are 0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.75, 1.0, 2.0, 3.0, 4.0, 6.0, 8.0, 10, 15, 20, 25μM, at this time, use a fluorescence spectrometer to test the fluorescence emission spectrum of the carbon quantum dot fluorescent probe solution in response to morin (excitation line at 345nm). Such as figure 2 As shown, with the continuous addition of morin, the emission peak at 460nm is continuously weakened, and the emission peak at 550nm is continuously enhanced. At the same time, if image 3As shown,...

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Abstract

A carbon quantum dot fluorescent probe, a test paper and the application thereof in the detection of morin belong to the technical field of fluorescent probes. Prepare 8-15mM polyethyleneimine solution with twice-distilled water; then, take 1mL, 8-15mM polyethyleneimine solution and mix with 9mL double-distilled water, and transfer them to a hydrothermal reaction kettle, 150-150mM React at 180° C. for 8 to 15 hours; after the reaction, the reaction product is cooled to room temperature, and then transferred to a centrifuge tube to obtain a carbon quantum dot fluorescent probe solution. Further, take a double-circle qualitative filter paper and place it on a glass slide, soak the filter paper with the carbon quantum dot fluorescent probe solution, and then dry it naturally to obtain the carbon quantum dot fluorescent test paper. The invention has the characteristics of fast response time, simple operation, high sensitivity and no need for any detection instrument, and is of great significance to the research on the metabolism of morin and the detection of morin in food samples.

Description

technical field [0001] The invention belongs to the technical field of fluorescent probes, and in particular relates to a carbon quantum dot fluorescent probe, a test paper and its application in the detection of morin. Background technique [0002] Morin is a natural polyphenol. It widely exists in the leaves, fruits, stems and branches of Moraceae plants, and plays a certain therapeutic role in various human diseases, including diabetes, cancer, bacterial infection, high blood pressure, hypoglycemia, etc. In addition, morin can reduce the side effects of drugs without interfering with their normal functioning. Its own toxicity is also low, and it can be used for long-term administration. Therefore, the construction of the morin detection method is conducive to monitoring the content of morin in food and its metabolism in the human body, and plays a positive role in the application of morin in the human body. The existing detection methods such as liquid chromatography a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/65G01N21/64B82Y20/00B82Y30/00
CPCB82Y20/00B82Y30/00C09K11/65G01N21/6428G01N2021/6432
Inventor 吴玉清于旭李洪伟
Owner JILIN UNIV
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