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A kind of preparation method of compound factor of mesenchymal stem cell

A technology of mesenchymal stem cells and composite factors, which is applied in the field of mesenchymal stem cell factor preparation, can solve the problems such as the inability to meet the large demand for skin repair products, the low efficiency of MSCs, and the long preparation process, so as to shorten the preparation time and increase the secretion volume. , the effect of accelerating cell proliferation

Active Publication Date: 2021-04-23
HUNAN YUANPIN CELL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] With the continuous development of biotechnology, stem cell factors have been applied to skin repair products, but the preparation methods of most products are too complicated, the efficiency of obtaining MSCs is low, and the content of cytokines secreted by mesenchymal stem cells is small, which cannot meet High demand in skin repair products, and the preparation process takes a long time

Method used

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  • A kind of preparation method of compound factor of mesenchymal stem cell
  • A kind of preparation method of compound factor of mesenchymal stem cell
  • A kind of preparation method of compound factor of mesenchymal stem cell

Examples

Experimental program
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Effect test

Embodiment 1

[0049] A preparation method of repair liquid containing mesenchymal stem cell factor

[0050] The method specifically includes the following steps:

[0051] A. Preparation of compound factors of mesenchymal stem cells:

[0052] (1) Isolation and culture of umbilical cord Huatong jelly mesenchymal stem cells: Aseptically take healthy human umbilical cord tissue, soak in 75% ethanol for 1 min, cut off 1 cm from both ends of the umbilical cord tissue, and wash the rest with normal saline until clear , cut into small pieces of 2cm, washed with normal saline for 3 times, transferred the umbilical cord tissue to a new culture dish, added normal saline to the culture dish to submerge 1 / 2 of the umbilical cord tissue, cut along the venous cavity of the umbilical cord tissue, and spread it flat Remove 1 vein, 2 arteries and amniotic membrane, and take the jelly between blood vessels and between blood vessels and adventitia, that is, Huatong glue, and cut Huatong glue to 1mm 3 size, t...

Embodiment 2

[0065] A preparation method of repair liquid containing mesenchymal stem cell factor

[0066] The method specifically includes the following steps:

[0067] A. Preparation of compound factors of mesenchymal stem cells:

[0068] (1) Isolation and culture of umbilical cord Huatong jelly mesenchymal stem cells: Aseptically take healthy human umbilical cord tissue, soak in 75% ethanol for 1 min, cut off 1 cm from both ends of the umbilical cord tissue, and wash the rest with normal saline until clear , cut into small pieces of 2cm, washed with normal saline for 3 times, transferred the umbilical cord tissue to a new culture dish, added normal saline to the culture dish to submerge 1 / 2 of the umbilical cord tissue, cut along the venous cavity of the umbilical cord tissue, and spread it flat Remove 1 vein, 2 arteries and amniotic membrane, and take the jelly between blood vessels and between blood vessels and adventitia, that is, Huatong glue, and cut Huatong glue to 1mm 3 size, t...

Embodiment 3

[0081] A preparation method of repair liquid containing mesenchymal stem cell factor

[0082] The method specifically includes the following steps:

[0083] A. Preparation of compound factors of mesenchymal stem cells:

[0084] (1) Isolation and culture of umbilical cord Huatong jelly mesenchymal stem cells: Aseptically take healthy human umbilical cord tissue, soak in 75% ethanol for 1 min, cut off 1 cm from both ends of the umbilical cord tissue, and wash the rest with normal saline until clear , cut into small pieces of 2cm, washed with normal saline for 3 times, transferred the umbilical cord tissue to a new culture dish, added normal saline to the culture dish to submerge 1 / 2 of the umbilical cord tissue, cut along the venous cavity of the umbilical cord tissue, and spread it flat Remove 1 vein, 2 arteries and amniotic membrane, and take the jelly between blood vessels and between blood vessels and adventitia, that is, Huatong glue, and cut Huatong glue to 1mm 3 size, t...

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Abstract

The invention discloses a preparation method of a repair liquid containing mesenchymal stem cell factors, and relates to the technical field of skin care products. The method comprises two steps of preparation of mesenchymal stem cell compound factor and preparation of a repair solution containing mesenchymal stem cell factor, and the prepared repair solution includes mesenchymal stem cell compound factor, sodium hyaluronate, mannitol, nicotinamide, sugar beet Alkali, Oligopeptide‑3, Dimethicone, Azone, and Prunella vulgaris Extract. Through the overall optimization of the preparation method, the secretion of mesenchymal stem cell factors is increased, and the cell proliferation speed is accelerated; the preparation method is simpler, the preparation time is shortened, and it is applicable to large-scale production. In addition, the repair liquid produced by this method has the effect of repairing acne marks, sunburn, acne, etc., and is mild and non-irritating, and has no adverse reactions when used.

Description

technical field [0001] The invention relates to the technical field of skin care products, in particular to a preparation method of mesenchymal stem cell factor. Background technique [0002] The skin has the functions of blocking the invasion of foreign bodies and pathogens and preventing the loss of body fluids. It is one of the largest organs in the human body and plays an important role in protecting the human body. With the increase of age and the deterioration of the living environment, the metabolism of the skin decreases, and the function of absorbing various beneficial elements decreases. The skin will appear dry, rough, peeling, etc., and scars such as acne marks, sunburn, and acne are difficult to eliminate. When repairing scar-like skin problems, some existing skin repair products will cause a slight tingling sensation when used, and occasionally allergic and other adverse reactions will occur for people with special constitutions. [0003] At present, a large n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/99A61K8/9789A61K8/891A61K8/73A61K8/67A61K8/34A61K8/44A61K8/64A61P17/02A61Q19/00
CPCA61K8/345A61K8/44A61K8/64A61K8/675A61K8/735A61K8/891A61K8/99A61K2800/72A61K2800/82A61Q19/00A61Q19/004A61K8/9789A61P17/02
Inventor 王成郑春兵文乐郭彬王健
Owner HUNAN YUANPIN CELL TECH CO LTD
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