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Compound with antibacterial activity as well as preparation method and application thereof

A technology of bacteriostatic activity and compound, applied in the field of new compound with bacteriostatic activity and preparation thereof, can solve problems such as insecurity, and achieve the effect of strong inhibitory effect

Active Publication Date: 2020-01-14
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a new anti-phytopathogenic fungal active compound against the shortcomings of unsafe chemical pesticides and fewer types of microbial pesticides; another purpose of the present invention is to provide the control effect of the active compound on plant diseases

Method used

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  • Compound with antibacterial activity as well as preparation method and application thereof
  • Compound with antibacterial activity as well as preparation method and application thereof
  • Compound with antibacterial activity as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1: (Isolation and Screening of Amylase Streptomyces Chromogenes D)

[0030] The present invention obtained the amylase chromogenic Streptomyces chromogen from the soil sample collected in Tianmu Mountain, Lin'an, Zhejiang Province in August 2006, through the steps of separation, cultivation, fermentation, activity determination and screening of effective strains for inhibiting plant fungal disease pathogens. diastatochromogenes) D, preservation.

[0031] Determination of antibacterial activity of amylase Streptomyces chromogenes (S.diastatochromogenes) D: Take 1 mL of its fermentation supernatant in a sterile petri dish, mix it with 9 mL of PDA medium cooled to 50 °C quickly, and put it in each Place a 4mm-diameter cake of cucumber solanum blight or tomato botrytis cinerea on the medium plane, connect the cake to the center of the petri dish (the diameter of the petri dish is 9cm), and culture it in an incubator at 28°C for 36 hours , with sterile water trea...

Embodiment 2

[0034] Embodiment 2: (preparation method of amylase Streptomyces chromogenes D fermentation metabolite)

[0035] Follow these steps:

[0036] (1) Bacteria test tube slant activation culture: the slant medium is Gaoshi No. 1 medium, and its components and content are: K 2 HPO 4 0.5g, KNO 3 1g, MgSO 4 0.5g, 20g soluble starch, 0.5g NaCl, FeSO 4 0.01g, 20g of agar, add water to 1L after dissolving; pick a little amylase spores of Streptomyces chromogenicum D with an inoculation loop under aseptic conditions, and put them into the sterilized Gaoshi No. 1 medium test tube (15×150mm ), placed in an incubator at 28°C±1°C, activated for 96 hours, until the slant of the test tube is covered with spores;

[0037] (2) Fermentation culture: The fermentation medium contains 5g of glucose, 4g of yeast extract, 6g of malt extract, and CaCO per 1000mL. 3 5g, NaCl2 g, the balance is water; 60mL fermentation broth per 300mL triangular bottle, after preparation, sterilize at 121°C for ...

Embodiment 3

[0046] Embodiment 3: (tetramycin Z is to the inhibition of cucumber solanum blight or tomato Botrytis cinerea)

[0047] (1) Tetramycin Z solutions of various concentrations are prepared;

[0048] (2) Determination of antibacterial activity: Take 1 mL of tetramycin Z solution of each concentration in a sterile petri dish, and quickly mix it with 9 mL of PDA medium cooled to 50 ° C. After cooling, place 1 mL on each medium plane. A bacterium cake of cucumber solanum blight or tomato cinerea cinerea with a diameter of 4mm, the bacterium cake is connected to the center of the petri dish (the diameter of the petri dish is 9cm), puts in the incubator and cultivates for 36h at 28°C, with conventional chemicals and sterile water The treatment was repeated 3 times as a control; the colony diameter was determined by the cross method, and the inhibition rate was calculated by the following formula:

[0049]

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Abstract

The invention discloses a compound with bacteriostatic activity as well as a preparation method and an application thereof. The compound with antibacterial activity is obtained by separating and purifying a product obtained by specific fermentation culture of streptomyces diastatochromogenes D. A preservation number of the Streptomyces diastatochromogenes D is CGMCC No. 2060. The streptomyces diastatochromogenes D disclosed by the invention is fermented and extracted to obtain an active compound Tetramycin Z with an inhibiting effect on part of plant fungal disease pathogenic bacteria, and thecompound has a relatively strong inhibiting effect on common plant pathogenic fungi; and the compound provides a new approach for development of new agricultural bactericides in the future.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a new compound with antibacterial activity and its preparation method and application. Background technique [0002] Among the crop diseases caused by different pathogenic bacteria, about 70% of the diseases are caused by fungi. Polyene macrolide antibiotics are a class of antibiotics with multiple conjugated double bonds, and their ultraviolet absorption is finger-like, which is very distinctive. According to the number of conjugated double bonds, polyene antibiotics can be divided into five types: triene, tetraene, pentacene, hexaene, and heptacene. They usually have strong antifungal activity and a wide antifungal spectrum. Main pathogenic fungi such as cucumber blight (Rhizoctoniasolani), tomato cinerea (Botrytis cinerea), tomato early blight (Alternayiasolani), wheat scab (Fusarium graminearium) and cucumber wilt (F.oxysporum); on food and feed Common molds such as Asperg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/08C12P19/62A01P3/00C12R1/525
CPCC07H17/08C12P19/62
Inventor 申屠旭萍宋阳刘光富许益鹏俞晓平
Owner CHINA JILIANG UNIV
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