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Preparation method of composite IgY antigen against propionibacterium acnes and staphylococcus and application of composite IgY antigen

A technology of Propionibacterium acnes and Staphylococcus, which is applied in the direction of bacterial antigen components, antibacterial immunoglobulin, antibacterial drugs, etc., can solve the problems of bacterial flora imbalance, bacterial drug resistance, etc., and achieve rapid onset and low dosage less, better effect

Inactive Publication Date: 2020-01-14
南京道大药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, secondary staphylococcal infection is the main cause of permanent skin lesions, and long-term antibiotic use also leads to bacterial dysbiosis and bacterial resistance

Method used

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  • Preparation method of composite IgY antigen against propionibacterium acnes and staphylococcus and application of composite IgY antigen
  • Preparation method of composite IgY antigen against propionibacterium acnes and staphylococcus and application of composite IgY antigen
  • Preparation method of composite IgY antigen against propionibacterium acnes and staphylococcus and application of composite IgY antigen

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preparation example Construction

[0041] The preparation method and application of the anti-Propionibacterium acnes and Staphylococcus composite IgY antigen provided in this example include the following methods:

[0042] Step 1: Preparation of immunogen. Take Propionibacterium acnes and cell fragments, and mix with Staphylococcus aureus and cell fragments, and then mix with Freund's complete adjuvant and Freund's incomplete adjuvant, stir and emulsify to prepare Water-in-oil Freund's complete adjuvant and Freund's incomplete adjuvant Propionibacterium acnes and Staphylococcus immunogens;

[0043] Step 2: Immunization, inject Freund's complete adjuvant immunogen into laying hens;

[0044] Step 3: Preparation of anti-Propionibacterium acnes and Staphylococcus composite IgY antibody extracts, collect the eggs of the immune hens, take the egg yolk and mix with distilled water, and adjust the acid-base and take the supernatant to obtain the anti-Propionibacterium and Staphylococcus complex IgY antibody extract.

[0045] ...

Embodiment 1

[0051] The production process for preparing 600 grams of crude extract of anti-P. acnes and Staphylococcus complex IgY antibody:

[0052] Step 1: Preparation of immunogen

[0053] (1) Propionibacterium acnes and Staphylococcus aureus and white staphylococcus isolated from patients with pyogenic acne, which are amplified and cultured with a meat medium, and 10% fresh sheep blood tryptone broth medium is expanded Increased culture of Staphylococcus aureus and white staphylococcus, which were identified as Propionibacterium acnes and Staphylococcus aureus and white

[0054] (2) The above-mentioned bacterial fragments are inactivated by carbolic acid, and the bacteria are ultrasonically pulverized with an ultrasonic pulverizer for 30 minutes, so that the bacterial fragments are crushed and broken into small and / or molecular fragments;

[0055] (3) Propionibacterium acnes and Propionibacterium acnes mycelial fragments are mixed in 65:25:10 parts of aureus and white staphylococcus and aureu...

Embodiment 2

[0070] The production process of 600 grams of crude extract of anti-Propionibacterium acnes and Staphylococcus complex IgY antibody:

[0071] Step 1: Preparation of immunogen

[0072] (1) Propionibacterium acnes and Staphylococcus aureus and white staphylococcus isolated from patients with pyogenic acne, which were amplified and cultured with a meat medium, and 10% fresh sheep blood tryptone broth medium was expanded Increased culture of Staphylococcus aureus and White Staphylococcus, which was identified as Propionibacterium acnes and Staphylococcus aureus and White;

[0073] (2) The above-mentioned bacterial fragments are inactivated by carbolic acid. In addition, ultrasonically pulverize the bacterial fragments with an ultrasonic pulverizer for 30 minutes, so that the bacterial fragments are crushed and broken into small and / or molecular fragments;

[0074] (3) Propionibacterium acnes and Propionibacterium acnes mycelial fragments are mixed in 65:25:10 quantities of golden yellow ...

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Abstract

The invention discloses a preparation method of a composite IgY antigen against propionibacterium acnes and staphylococcus and an application of the composite IgY antigen, and belongs to the technicalfield of resistance against propionibacterium acnes and staphylococcus. The preparation method comprises the following steps: step 1, preparation of immunogens: mixing propionibacterium acnes and fragments thereof with staphylococcus aureus, staphylococcus albus and fragments thereof, and then mixing with a Freund's complete adjuvant and a Freund's incomplete adjuvant respectively, conducting emulsifying by stirring to obtain the water-in-oil Freund's complete adjuvant and Freund's incomplete adjuvant immunogens against propionibacterium acnes and staphylococcus; step 2, immunization: injecting a Freund's complete adjuvant immunogen to laying hens. The specific composite IgY antigen against propionibacterium acnes and staphylococcus prepared by the invention is applied locally on face skin and whole body skin, and has advantages of rapid action, less dosage, good curative effect and no damage on normal micro-ecological environments of mucosa and skin without limitations and side effects of systemic administration.

Description

Technical field [0001] The invention relates to the preparation and application of anti-acne IgY, in particular to a preparation method and application of an anti-Propionibacterium acnes and Staphylococcus composite IgY antigen, and belongs to the technical field of anti-Propionibacterium acnes and Staphylococcus. Background technique [0002] Propionibacterium acnes Propionibacterium acnes, also known as Acne bacillus, Propionibacterium acnes, belongs to the genus Bacteria, Actinobacteria, Class, Subclass, Order, Propionibacterium suborder, Family, and Propionibacterium. It is a kind of The relatively slow-growing typical gram-positive anaerobes are short rod-shaped, parasitic in cells, belong to the normal flora of the skin, and generally reside in the hair follicles and sebaceous glands of the skin. The genes of P. acnes control the production of enzymes that break down skin and immunogenic proteins. Propionibacterium acnes lives on the fatty acids in the pores. When the pore...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61K39/395A61K39/085A61K39/05A61P17/10A61P31/04A61P17/00A61P17/02A61P31/22A61P31/10C07K16/12C07K16/02
CPCA61K39/395A61K39/085A61K39/05A61K9/0014A61P17/10A61P31/04A61P17/00A61P17/02A61P31/22A61P31/10C07K16/1271C07K16/1267C07K16/02A61K2039/55566A61K2039/70C07K2317/11
Inventor 付宇
Owner 南京道大药业有限公司
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