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Carboxymethyl chitosan/sodium alginate nano hydrogel as well as preparation method and application

A carboxymethyl chitosan and nano-hydrogel technology, which is applied in pharmaceutical formulations, peptide/protein components, medical preparations with non-active ingredients, etc. Gel research and other issues, to achieve the effect of good protein entrapment capacity, good biosafety and good dispersibility

Active Publication Date: 2019-12-31
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] However, carboxymethyl chitosan-sodium alginate is a natural polymer substance, it is difficult to form a nanoscale gel, and there is currently no research on its nanoscale hydrogel

Method used

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  • Carboxymethyl chitosan/sodium alginate nano hydrogel as well as preparation method and application
  • Carboxymethyl chitosan/sodium alginate nano hydrogel as well as preparation method and application
  • Carboxymethyl chitosan/sodium alginate nano hydrogel as well as preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Prepare a solution of carboxymethyl chitosan (OCMC) and sodium alginate (SA) with a concentration of 2.5mg / mL. The two are ultrasonicated under the conditions of ultrasonication for 2.5s, pause for 2.5s, cycle for 60min, and power of 630w, and then press The volume ratio is 1:1, and the mixture is sonicated under the conditions of ultrasonication for 2.5s, pause for 2.5s, circulation for 30min, and power 630w.

[0051] The mixture was dropped into ethyl acetate to make water phase: organic phase = 1:9 (v:v), and mechanically stirred for 2 hours at 600 rpm. Afterwards, the product was transferred to a round-bottomed flask, and vacuum-rotated at 35° C. to remove the organic solvent to obtain an aqueous phase. Add calcium chloride solution with a concentration of 1 mg / mL according to the molar ratio of calcium ions and sodium alginate at a ratio of 1:1, stir magnetically, and cross-link at room temperature for 2 h. The product was dialyzed in deionized water for 2 days, a...

Embodiment 2

[0058] After the L929 cells were revived, cultured and passaged, the original medium was discarded, and 2-3mL PBS was added to wash twice. Add 1 mL of trypsin in PBS solution (concentration: 2.5 mg / mL, use after filter sterilization) for digestion for 3 min (put in an incubator at 37°C). After the digestion is completed, observe under the microscope, and the digestion is complete when the cells become spherical. Add 3mL medium by pipetting. Transfer to a centrifuge tube for centrifugation (1000 rpm, 5 min). Discard the supernatant, add 6 mL medium and blow up. After pipetting evenly, transfer to culture flasks, and add 3mL cell suspension to each culture flask.

[0059] After two passages, the supernatant was discarded after the cells were centrifuged, and 5 mL of medium was added again, and mixed by pipetting. Take a drop into the counting plate, dilute according to the result, and adjust the concentration to 1.5-1.8×10 4 individual / mL. After using the counting board, w...

Embodiment 3

[0064] Prepare OCMC and SA solution with a concentration of 2.5 mg / mL; prepare a BSA solution with a concentration of 5 mg / mL, take 4 mL of OCMC solution (containing 10 mg of OCMC), and add 4 (8, 12, 16) mL of BSA solution (ie OCMC:SA:BSA=1:1:2(4,6,8)(m:m:m)), ultrasonic for 2.5s, pause for 2.5s, power 630w, disperse for 10min. Then drop 4mL of SA solution (containing 10mg of SA) into it, sonicate for 2.5s, pause for 2.5s, power 630w, and disperse for 20min.

[0065] Drop the mixture into ethyl acetate, the volume ratio of aqueous phase: organic phase is 1:9, and react for 2 hours under mechanical stirring at 600rpm; the reaction system is vacuum-dried to remove the organic solvent, and the remaining aqueous phase is divided into 50mL centrifuge tubes , Centrifuge at 36400g for 30min at room temperature. Take the supernatant to measure its volume, and measure the absorbance at 280nm, and determine the BSA concentration by comparing with the BSA standard curve.

[0066] Calcu...

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Abstract

The invention provides carboxymethyl chitosan / sodium alginate nano hydrogel. Carboxymethyl chitosan and sodium alginate form a network interpenetration structural body by ion crosslinking, the networkspace of the network interpenetration structural body can entrap substances. The invention further discloses a preparation method of the nano hydrogel. The preparation method comprises the steps as follows: mixing carboxymethyl chitosan and sodium alginate, and performing ultrasonic treatment to obtain a mixed solution; dropping the mixed solution in ethyl acetate, and performing mechanical stirring and vacuum rotary evaporation to remove an organic phase to obtain an aqueous phase solution; adding a calcium chloride solution to the aqueous phase solution for a crosslinking reaction at room temperature; dialyzing the crosslinked aqueous phase solution, and carrying out freeze-drying to obtain the final product, namely, the carboxymethyl chitosan / sodium alginate nano hydrogel. The constructed nano hydrogel based on carboxymethyl chitosan and sodium alginate shows good protein entrapment capacity, has good biocompatibility and pH sensitivity, and has broad application prospects in oralmedication.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, in particular to a carboxymethyl chitosan / sodium alginate nano hydrogel. Background technique [0002] Type 1 diabetes is generally thought to be related to the destruction of insulin-producing pancreatic beta cells, and the immediate need for exogenous insulin is also a hallmark of type 1 diabetes, which requires lifelong treatment. Insulin is still the main drug in the treatment of type 1 diabetes. Due to its nature as a protein drug, invasive parenteral treatment (ie, injection treatment) is still a widely used delivery method. Its effectiveness is limited by the patient's needle phobia, pain, skin swelling, allergic reactions, common infections, and the stress of living on long-term insulin therapy. Also, many patients still suffer from hypoglycemic episodes despite simpler and more effective blood glucose monitoring measures. The incidence of the above adverse reactions varies, and it...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K47/36A61K38/28A61K38/38A61P3/10C08J3/24C08J3/075C08L5/08C08L5/04
CPCA61K9/0053A61K9/06A61K38/28A61K38/385A61K47/36A61P3/10C08J3/075C08J3/246C08J2305/04C08J2305/08C08J2405/04C08J2405/08
Inventor 常菁张海斌陈晓彤张旺旺孙乐韩宝芹
Owner OCEAN UNIV OF CHINA
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