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Application of astragaloside IV in reduction of nanometer zinc oxide-induced neurotoxicity

A technology of nano-zinc oxide and astragaloside IV is applied in nervous system diseases, medical preparations containing active ingredients, organic active ingredients, etc. It can solve problems such as no research reports on astragaloside IV, and achieve good medicinal prospects, Good pharmacological effect and low toxicity

Inactive Publication Date: 2019-12-27
SHANXI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no research reports on the application of astragaloside IV in neuroprotective drugs

Method used

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  • Application of astragaloside IV in reduction of nanometer zinc oxide-induced neurotoxicity
  • Application of astragaloside IV in reduction of nanometer zinc oxide-induced neurotoxicity
  • Application of astragaloside IV in reduction of nanometer zinc oxide-induced neurotoxicity

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0032] Astragalus has protective effects against neurotoxicity induced by ZnONPs

[0033] CCK method was used to detect the survival rate of SH-SY5Y cells, and Annexin V-FITC / PI was used to detect cell apoptosis: as follows, different concentrations of ZnONPs (0-7 μg / mL, 8 concentrations), different concentrations of Astragalus (0, 10 , 30, 50, 80, 100, 200 μg / mL); nano-zinc oxide (7 μg / mL) and different concentrations of Astragalus acted on SH-SY5Y cells for 24 hours to detect the effect on cell viability. The concentration of Astragalus membranaceus (30, 80 μg / mL) and nano zinc oxide (6 μg / mL) were selected to intervene the cells for 24 hours, and the apoptosis was detected. Experimental results such as figure 1 As shown in A, in the figure, the label AM is Radix Astragali. After 24 hours of different concentrations of ZnONPs acting on the cells, the cells have obvious inhibitory effect. When the nano-zinc oxide is 7 μg / mL, the cell survival rate is about 65%, 8 μg / mL , th...

example 2

[0035] Astragaloside IV has a protective effect on neurotoxicity induced by ZnONPs

[0036] Adopt CCK8 method to detect SH-SY5Y cell survival rate: specifically as follows, implementation method is the same as example 1, adopts astragaloside IV of different concentrations, astragalus polysaccharide, calycosin (0,1,10,30,50,100,200,300 μ g / mL, 8 concentrations) to detect the impact on cell viability; select the concentration range (0-30 μg / mL) with cell viability greater than 80% to carry out the protection experiment, and the implementation method is the same as in Example 1. Experimental results such as image 3 As shown in A, different concentrations of astragaloside IV, astragalus polysaccharide, and calycosin acted on the cells for 24 hours had obvious inhibitory effect on the cells. About 85.3%, 96.8%, 83.7%, and at 300 μg / mL, the cell viability was about 69.4%, 42.8%, 42.6%, respectively. Choose the concentration of the three components at 1-30 μg / mL to intervene with...

example 3

[0038] Astragaloside IV can induce autophagy

[0039] Western blot method was used to detect the content changes of autophagy-related proteins in SH-SY5Y cells before and after the treatment of astragaloside IV. , 12h) and ZnONPs (5μg / mL) co-acting cells, using Western blot method to observe the changes in the content of autophagy proteins in the cells; 15, 30 μg / mL), ZnONPs (5 μg / mL), the two work together to intervene nerve cells respectively, and observe the changes in the content of autophagy proteins in cells by Western blot. Such as Figure 4 As shown, by Western blot detection of LC3 type conversion, the change of p62 found that compared with the effect of ZnONPs alone, the intracellular p62 showed a down-regulation trend, the expression of LC3-Ⅱ type was up-regulated, and Beclin1 showed an up-regulation trend, which proved that Astragalus A The joint action of glycosides and ZnONPs can enhance the induction of autophagy, and the degree of autophagy increases with the...

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Abstract

The invention discloses novel application of astragaloside IV and application of astragaloside IV in reduction of nanometer zinc oxide-induced neurotoxicity. The astragaloside IV is one of the main components of astragalus. It is shown through tests that ZnONPs-induced never cell toxicity can be prevented through induced autophagy of astragaloside IV, damaged mitochondria can be further protectedthrough PINK1 / Parkin pathway-induced mitochondrial autophagy, and the intracellular ROS content increase, reduction of MMP and calcium ion homeostasis imbalance and mitochondrial mass reduction whichare caused by ZnONPs can be reversed. It is determined that a protective effect on nanometer zinc oxide-induced neurotoxicity can be achieved by astragalus, and the protective effect of astragalosideIV on neurotoxicity is achieved; and the protective mechanism of astragaloside IV is studied through in-vitro experiments, and a foundation is laid for researches and development of new neuroprotective drugs.

Description

technical field [0001] The invention relates to a new application of astragaloside IV, in particular to an application of astragaloside IV in reducing neurotoxicity induced by nanometer zinc oxide. Background technique [0002] In recent years, zinc oxide nanoparticles (ZnONPs), as a widely used nanomaterial, have been used in oral medical materials, food additives, dermatological drugs, cosmetics and other fields. ZnO nanoparticles have pH-dependent ion release properties and are one of the most toxic metal oxide nanoparticles. Under acidic conditions, nano-zinc oxide is easier to dissolve and release zinc ions. Therefore, the endocytosed zinc oxide particles are in the acidic vesicles of lysosomes, which can dissolve and release zinc ions. An appropriate amount of zinc ions shows some positive physiological effects, while high Concentrations of nano-ZnO enter cells and cause some cytotoxic effects. People are exposed to more and more nano-applications. Nano-zinc oxide ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/481A61K31/7048A61P25/00
CPCA61K31/7048A61K36/481A61P25/00
Inventor 梁泰刚李青山王丽伟孙丽倩闫超群杨聪聪梁马丹段治宇
Owner SHANXI MEDICAL UNIV
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