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Method of quickly detecting chlorpromazine hydrochloride in fodder

A technology of chlorpromazine hydrochloride and feed, applied in the direction of measuring devices, analysis materials, material excitation analysis, etc., can solve the problems of long test cycle and complicated pre-treatment, and achieve the effect of shortening the detection cycle, improving sensitivity, and improving detection sensitivity

Inactive Publication Date: 2019-12-24
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The biggest drawback of the two methods is that the pre-processing is complex and the test period is long, and large-scale equipment is required, making these two methods unsuitable for real-time and rapid on-site detection. Therefore, a method with high sensitivity, simplicity, accuracy, Low-cost identification methods are necessary

Method used

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  • Method of quickly detecting chlorpromazine hydrochloride in fodder
  • Method of quickly detecting chlorpromazine hydrochloride in fodder
  • Method of quickly detecting chlorpromazine hydrochloride in fodder

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A method for rapid detection of chlorpromazine hydrochloride in feed, comprising the steps:

[0028] (1) Weigh 1g of feed, crush it and place it in a centrifuge tube, add 2ml of 0.1% sodium hydroxide in ethyl acetate, oscillate ultrasonically for 1min, centrifuge at 4000rpm for 1min, take the supernatant and add 1mL distilled water, oscillate, and centrifuge at 4000rpm for 1min , the obtained supernatant is the test solution.

[0029] (2) Take 200 μL of the solution to be tested in the test bottle, add 100 μL of the surface enhancer, and then add 100 μL of coagulant, and shake for 5 seconds to obtain a mixed solution. The surface enhancer is a nano-gold colloid solution, and the coagulation accelerator is a sodium chloride solution with a concentration of 0.1 g / mL.

[0030] The preparation method of nanometer gold sol: get 200mL0.01% chloroauric acid (AuCl 3 ·HCl·4H 2 O) The aqueous solution is heated to boiling, and 2.0 mL of 1% trisodium citrate (Na 3 C 6 h 5 o ...

Embodiment 2

[0038] The surface enhancer is a nano-silver colloidal solution, and the specific preparation method is:

[0039] Take 100mL1.0 mM silver nitrate (AgNO 3 ) aqueous solution is heated to boiling, and 2.5mL of 1% trisodium citrate (Na 3 C 6 h 5 o 7 ) aqueous solution, continue to boil for 1 hour, the solution turns gray-green, if the solution is less than 100mL after cooling, add distilled water to 100mL. The water used for the preparation of enhanced reagents is the first-class water specified in GB / T 6682.

[0040] Other steps are the same as in Example 1.

[0041] In this embodiment, the feed is 4% medium pig premix, and the judgment result obtained is negative, that is, it does not contain chlorpromazine hydrochloride or is lower than 100ppb.

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Abstract

The invention discloses a method of quickly detecting chlorpromazine hydrochloride in fodder. The method comprises the steps that (1) a sample is extracted, and a to-be-detected liquid is obtained; (2) the to-be-detected liquid is added into a surface enhancer, then a coagulant is added, and a mixed liquid is obtained after uniform mixing; (3) a Raman spectrometer is adopted to perform Raman spectrum determination on a blank sample containing a standard substance, and a chlorpromazine hydrochloride standard substance database is established; (4) the Raman spectrometer is adopted to perform Raman spectrum determination on the mixed liquid; and (5) the Raman spectrometer automatically recognizes a negative / positive sample. Through the method, a pretreatment step in an analysis process can besimplified, the detection cycle can be shortened, the detection sensitivity can be improved, and the detection cost can be lowered.

Description

technical field [0001] The invention relates to a method for detecting chemical substances, in particular to a method for rapidly detecting chlorpromazine hydrochloride in feed. Background technique [0002] Chlorpromazine hydrochloride is a representative drug of thiaphenazines. It is a common sleeping, sedative, and hypnotic drug. It is a blocker of central dopamine receptors and acts on the central nervous system. It is mainly used as a tranquilizer in veterinary medicine. Adding such drugs to the feed can indirectly promote fattening and reduce the mortality of animals during transportation. If chlorpromazine hydrochloride enters the human body through animal food, it can cause poisoning after long-term accumulation. In large doses, it can cause human liver and kidney lesions, and even endanger life and health. [0003] At present, the detection methods of chlorpromazine hydrochloride in feed mainly include high-performance liquid chromatography (HPLC) and liquid chroma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 席广成李亚辉白桦
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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