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TPP mitochondria target astaxanthin emulsion and preparation method thereof

An astaxanthin and mitochondrial technology, which is applied in the field of TPP mitochondria-targeted astaxanthin emulsion and its preparation, can solve the problems of reducing the titer of functional factors, inability of functional factors, and inability to achieve targeted localization release, etc. The effect of reactive oxygen species level, excellent promotion effect and excellent macrophage viability

Inactive Publication Date: 2019-12-24
DALIAN POLYTECHNIC UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the emulsion constructed in the traditional way improves the stability of the functional factor, it cannot realize the targeted release function, so that the functional factor cannot be concentrated and efficiently distributed at the action site, reducing the potency of the functional factor

Method used

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  • TPP mitochondria target astaxanthin emulsion and preparation method thereof
  • TPP mitochondria target astaxanthin emulsion and preparation method thereof
  • TPP mitochondria target astaxanthin emulsion and preparation method thereof

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preparation example Construction

[0039] A preparation method of TPP mitochondria targeting astaxanthin emulsion, comprising the steps of:

[0040] S1. Preparation of casein emulsion: take casein and dissolve it in deionized water so that the final concentration of casein is 0.5mg / mL~1mg / mL to obtain casein aqueous solution; add MCT (medium chain triglyceride ), stirred at 500-1000rpm for 15-30min to obtain a mixed solution; the mixed solution was ultrasonically crushed, the ultrasonic frequency was 600-800W, the ultrasonic duration was 7-15min, and the temperature was 20-30°C to obtain a casein emulsion; wherein, The volume ratio of the casein aqueous solution to MCT is 9:1~4:1;

[0041] S2, preparation of TPP carboxyl activation solution: DMSO (dimethyl sulfoxide) is diluted with deionized water into a DMSO solution with a volume fraction of 5%; EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) was fully dissolved in DMSO solution with a volume fraction of 5%, stirred at 500-1000rpm for 10-1...

Embodiment 1

[0047] A TPP mitochondria-targeted astaxanthin emulsion, comprising the following components: casein 1mg / mL, MCT (medium chain triglyceride) 0.1mL / mL, DMSO (dimethyl sulfoxide) 0.04mL / mL, TPP (Triphenylphosphine bromide) 0.025mg / mL, EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) 0.025mg / mL, NHS (N-hydroxysuccinate imide) 0.015mg / mL, astaxanthin 0.05mg / mL, and the balance is water.

[0048] The preparation method of the TPP mitochondria targeting astaxanthin emulsion comprises the following steps:

[0049] S1. Preparation of casein emulsion: Weigh casein and dissolve it in deionized water so that the final concentration of casein is 1 mg / mL, and fully dissolve it in a water bath at 40°C for 30 minutes to obtain an aqueous casein solution; Add MCT (medium-chain triglycerides) drop by drop, fully stir at 800rpm for 30min, and then ultrasonically crush the emulsion at 700W at an ultrasonic frequency of 7min at a temperature of 25°C to obtain a casein emulsion ...

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Abstract

The invention discloses TPP mitochondria target astaxanthin emulsion. The TPP mitochondria target astaxanthin emulsion comprises components of 0.5-1mg / mL of casein, 0.1-0.2mL / mL of medium chain triglyceride, 0.02-0.04mL / mL of dimethyl sulfoxide, 0.025-0.05mg / mL of triphenyl phosphonium bromide, 0.025-0.05mg / mL of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride), 0.01-0.025mg / mL of N-hydroxysuccinimide, 0.05-0.1mg / mL of astaxanthin, and the balance water. During preparation of the emulsion, casein is used for entrapping astaxanthin, and the surface of the emulsion is subjected to triphenyl phosphonium bromide modification, so that the emulsion can be distributed in the mitochondria in a targeted manner. Nutrient entrapping is realized, besides, nutrients are in targeted positioning, the bioactivity is developed, and interfering and destroying of bioactivity by external environment are avoided.

Description

technical field [0001] The invention relates to the technical field of food and health food, in particular to a TPP mitochondria-targeted astaxanthin emulsion and a preparation method thereof. Background technique [0002] Astaxanthin is a terpene unsaturated compound, one of the most common carotenoids, which is distributed in the feathers of shrimp, crab, fish, algae, yeast, crustaceans and birds. Astaxanthin is mainly in the esterified state, but the astaxanthin in muscle, plasma and internal organs is mainly in the free state. Because the astaxanthin molecule contains a long conjugated unsaturated double bond structure, it has strong biological activities, including anti-oxidation, anti-lipid peroxidation activity, anti-inflammation, prevention of cardiovascular diseases, and immune regulation. However, it is easily damaged by environmental factors such as light, heat, oxygen and pH, and loses its biological activity, and its bioavailability is low. [0003] Functional...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/17A23L33/115
CPCA23L33/17A23L33/115
Inventor 谭明乾张雪迪铁珊珊李佳璇崔国馨
Owner DALIAN POLYTECHNIC UNIVERSITY
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