Method of obtaining human recombinant fibronectin by utilizing genetic engineering
A technology of fibronectin and protease, applied in the field of genetic engineering, can solve the problems of low biological activity and low amount of artificially extracted fibronectin
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[0037] 1. Artificially synthesized a recombinant human fibronectin fusion protein, and the fibronectin functional fragment in the recombinant fibronectin has a full length of 180 amino acids. The nucleotide sequence of recombinant human fibronectin after codon optimization is:
[0038] SEQ ID NO:2
[0039] 5’-ATGCACCACCACCACCACCACAATAGTGATTCCGAGTGCCCTCTTAGTCACGATGGCTATTGCCTTCACGATGGCGTCTGCATGTACATCGAAGCTTTGGACAAGTACGCTTGTAACTGTGTTGTTGGTTACATCGGTGAAAGATGTCAATACAGAGACTTGAAGTGGTGGGAATTGAGAGGTTCTGAAGGTTCTGAAGGTGAAGGTGGTTCTGAAGGTTCTGAAGGTGAAGGTGGCAGCGAAGGCAGCGAAGGCGAAGGCGGCAGCGAAGGCAGCGAAGGCGAAGGCGGTTCTGAAGGTTCTGAAGGTGAAGGTGGTTCTGAAGGTTCTGAAGGTGAAGGTATTACTTACGGTGAAACTGGTGGTAACTCTCCAGTTCAAGAATTTACTGTTCCAGGTTCTAAGTCTACTGCTACTATTTCTGGTTTGAAGCCAGGTGTTGATTACACTATTACTGTTTACGCTGTTACTGGTAGAGGTGACAGTCCTGCCAGTAGTAAACCTATCTCCATCAACTATAGGACCGAGATCGACAAACCTTAA-3’或
[0040] SEQ ID NO:3
[0041] 5’-ATGCACCACCACCACCACCACAATAGTGATTCCGAGTGCCCTCTTAGTCACGATGGCTATTGCCTTCACGATGGCGTCTGCATGTACATCGAAGCTTT...
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