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Nitrifying bacterium agent and preparation method thereof

A technology of nitrifying bacteria agent and nitrifying bacteria, applied in biochemical equipment and methods, chemical instruments and methods, methods of using microorganisms, etc., can solve the problems of toxic water quality, large fluctuations, slow growth of nitrifying bacteria, etc.

Inactive Publication Date: 2019-12-13
山东海景天环保科技股份公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Nitrifying bacteria grow slowly and require harsh living conditions. Leather wastewater has complex components, high ammonia nitrogen, high salt content, toxicity, and large fluctuations in water quality and water volume, which is easy to impact the nitrification reaction of the sewage treatment biochemical system. Nitrifying bacteria prepared by conventional methods agent, due to its poor tolerance to leather wastewater pollutants, the nitrification effect is greatly reduced

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  • Nitrifying bacterium agent and preparation method thereof
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preparation example Construction

[0036] The invention provides a kind of preparation method of nitrifying bacteria agent, comprises the following steps:

[0037] A) adopting the activated sludge in the aerobic tank as the bacterial source, using the nitrifying bacteria inorganic salt culture medium to carry out the enrichment culture, to obtain the enrichment culture bacterium liquid;

[0038] The components of the nitrifying bacteria inorganic salt culture medium include: ammonium sulfate, sodium carbonate, sodium chloride, magnesium sulfate, ferrous sulfate, trace element mother liquor and water;

[0039] The components of the trace element mother liquor include: zinc sulfate, cobalt chloride, manganese chloride, boric acid, sodium molybdate and water;

[0040] B) using the enriched culture liquid as a seed, and using high ammonia nitrogen wastewater to carry out gradient acclimatization culture;

[0041] C) After the gradient acclimation culture is completed, the multiplication culture is carried out to o...

Embodiment 1

[0099] (1) Take tannery A aerobic pool mud-water mixture (wastewater ammonia nitrogen 1000mg / L, total salt content 15000mg / L, aerobic tank ammonia nitrogen degradation rate 90-120mg / (L.d)) 1000L, settle naturally for 1h, discard the supernatant , add special inorganic salt culture medium for nitrifying bacteria (wherein, water is solvent, ammonium sulfate 2.5g / L, sodium carbonate 1.8g / L, sodium chloride 0.6g / L, magnesium sulfate 0.12g / L, ferrous sulfate 0.18g / L, trace element mother liquor 2ml / L; the components of trace element mother liquor include: zinc sulfate 0.1g / L, cobalt chloride 0.3g / L, manganese chloride 1.6g / L, boric acid 0.01g / L, sodium molybdate 0.3g / L), set the volume to 1000L, and then use a fan to aerate the nitrifying bacteria enrichment culture system, maintain dissolved oxygen 3.0-5.0mg / L, temperature 25°C, pH 7.0, and carry out enrichment culture. When the ammonia nitrogen content in the culture solution is less than 2mg / L, stop aeration, settle naturally, ...

Embodiment 2

[0104] (1) Take tannery B aerobic pool mud-water mixture (wastewater ammonia nitrogen about 2000mg / L, total salt content about 32000mg / L, aerobic tank ammonia nitrogen degradation rate 60mg / (L.d)) 1000L, natural settlement for 1h, discard the supernatant , add special inorganic salt culture medium for nitrifying bacteria (wherein, water is solvent, ammonium sulfate 5.0g / L, sodium carbonate 3.8g / L, sodium chloride 0.6g / L, magnesium sulfate 0.20g / L, ferrous sulfate 0.18g / L, trace element mother liquor 3ml / L; the components of trace element mother liquor include: zinc sulfate 0.2g / L, cobalt chloride 0.3g / L, manganese chloride 2.1g / L, boric acid 0.02g / L, sodium molybdate 0.3g / L), set the volume to 1000L, and then use a fan to aerate the nitrifying bacteria enrichment culture system, maintain dissolved oxygen at 3.0-5.0mg / L, temperature 25°C, pH 7.0, and carry out enrichment culture. When the ammonia nitrogen content in the culture solution is less than 2mg / L, stop the aeration, s...

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Abstract

The present invention relates to the technical field of environmental microorganisms and water treatments, and particularly relates to a nitrifying bacterium agent and a preparation method thereof. The preparation method of the nitrifying bacterium agent comprises: A) activated sludge in an aerobic pond is used as a source of bacteria and enriching and cultivating are conducted by using a nitrifying bacterium inorganic salt culture medium to obtain enriched culture bacterium liquid; the nitrifying bacterium inorganic salt culture medium comprises the following components: ammonium sulfate, sodium carbonate, sodium chloride, magnesium sulfate, ferrous sulfate, trace element mother liquid and water; and the trace element mother liquid comprises the following components of zinc sulfate, cobalt chloride, manganese chloride, boric acid, sodium molybdate and water; B) the enriched culture bacterium liquid is used as a seed and high ammonia nitrogen wastewater is used for gradient domestication culture; and C) after the gradient domestication culture is completed, expanding culture is performed to obtain the nitrifying bacterium agent. The prepared nitrifying bacterium agent has relativestrong tolerance in the high ammonia nitrogen wastewater and high in nitrifying activity.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms and water treatment, in particular to a nitrifying bacteria agent and a preparation method thereof. Background technique [0002] Tannery sewage is one of the important pollution sources of water environment pollution, and it is also one of the "three major wastewaters" (papermaking wastewater, printing and dyeing wastewater, and tannery wastewater). The protein in the leather raw material animal skin will be separated during the processing process, and its continuous decomposition and ammoniation in the wastewater will produce a large amount of ammonia nitrogen. A large amount of ammonium salt is used in processes such as tanning, neutralization and dyeing, so the content of ammonia nitrogen in tanning wastewater is very high, which puts a huge pressure on wastewater treatment. [0003] At present, the treatment methods of ammonia nitrogen wastewater mainly include physical ...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/36C02F3/34C02F3/02C02F103/24
CPCC02F3/02C02F3/34C02F2103/24C12N1/00C12N1/36Y02W10/10
Inventor 燕锡尧张英薛飞李川川
Owner 山东海景天环保科技股份公司
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