Application of venenum bufonis polypeptide as medicine penetration enhancer
A technology of toad venom polypeptide and penetration enhancer, which is applied in the field of medicine, and can solve problems such as unclear efficacy of polypeptide substances, and achieve the effects of enhancing anti-inflammatory and analgesic effects, promoting penetration, and improving anti-inflammatory and analgesic capabilities
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[0021] 1. Materials and methods
[0022] 1.1 Reagents and instruments
[0023] Acetonitrile (HPLC grade) and methanol (HPLC grade) were purchased from Merck, Germany; formic acid was purchased from Sigma-Aldrich, USA. Standard compounds, bufafalin (118981–201501, ≥99.2%), bufafagenin (110718-201809, ≥99.6%), cinobufagin (110803-201408, ≥99.6%) were purchased from China Food and Drug General Administration of Supervision and Administration (Beijing, China), Sand Bufalin (180427, ≥98%), and Bufatalin (180118, ≥98%), Cinobufafeline (180318, ≥98%), Bufatalin (180419, ≥98%), and Yuanhuabufenjing (180427, ≥98%) were purchased from Beijing Putian Tongchuang Biotechnology Co., Ltd. (China, Beijing). Deionized water was purified by a milliq water system from Millipore.
[0024] Agilent 1290 high-performance liquid chromatography, 1290 variable wavelength detector (VWD), Agilent Corporation of the United States; AB SCIEX triple quadrupole mass spectrometer 5500 liquid mass spectromet...
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