Application of spermidine in preparation of aortic aneurysm treatment drug

A technology for aortic aneurysm and abdominal aortic aneurysm, which is applied in the direction of pharmaceutical formulations, drug combinations, and medical preparations containing active ingredients. High sex, clear toxicology and pharmacology effect

Active Publication Date: 2019-12-10
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are medical reports about its weight loss (CN 109820844A) and treatment of nerve cell damage (CN107929270A) in the prior art, but it does not involve aneurysm-related applications

Method used

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  • Application of spermidine in preparation of aortic aneurysm treatment drug
  • Application of spermidine in preparation of aortic aneurysm treatment drug
  • Application of spermidine in preparation of aortic aneurysm treatment drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Model establishment: mouse abdominal aortic aneurysm induced by trypsin infusion can simulate the pathophysiological mechanism of human abdominal aortic aneurysm formation, and can also reflect extracellular matrix degradation, smooth muscle cell apoptosis, inflammatory response, oxidative stress and other lesions, the postoperative survival rate is about 90%, and the tumor formation rate after survival is 100%. It is a widely recognized classic abdominal aortic aneurysm model.

[0026] We randomly divided 40 male C57 mice aged 8-10 weeks into two groups, 20 in each group. The control group had a normal diet, and the experimental group added spermidine (concentration 3 mmol / L) to their drinking water. The abdominal aortic aneurysm was formed on the 14th day after modeling, and the diameter of the aneurysm was measured by laparotomy and compared with the initial diameter ( figure 1 ), defined as neoplasia when the expansion was more than 50%. After measuring the aneurys...

Embodiment 2

[0028] Histopathological evaluation

[0029] In order to further evaluate the therapeutic level of spermidine, we used section staining to evaluate the degree of vascular lesions at the histopathological level, and then evaluated the therapeutic effect of spermidine.

[0030] After tumor formation in the two groups of mice, we took aneurysm specimens for paraffin-embedded and fixed sections, and used staining techniques for histopathological evaluation. We used EVG staining to observe the staining of elastic fibers in blood vessels, Masson staining to observe the staining of collagen fibers and muscle fibers in blood vessels, and SMA staining to observe the staining of vascular smooth muscle ( Figure 4 ). The experimental results show that spermidine can significantly improve the degradation of elastic fibers, the destruction of muscle fibers and collagen fibers, and the reduction of smooth muscle cells in abdominal aortic aneurysms. It was suggested that spermidine improve...

Embodiment 3

[0033] Flow Cytometry Evaluation

[0034] In order to further verify the effect of spermidine on the infiltration of inflammatory cells in aneurysm tissue, we used flow cytometry to evaluate the proportion of inflammatory cells in aortic tissue. We first digested the aortic tissue into single suspension cells with digestive enzymes, then labeled the corresponding cells with fluorescent antibodies, and finally analyzed and quantified them by flow cytometry. We found that in the spermidine-treated group, the proportion of leukocytes in the aneurysm tissue was significantly reduced (CD45 + )(P Image 6 ), suggesting that spermidine reduced inflammatory cell infiltration in abdominal aortic aneurysms.

[0035] Since inflammatory monocytes in mouse blood are the precursors of inflammatory cells such as monocytes and macrophages in abdominal aortic aneurysms, we also evaluated circulating inflammatory monocytes in mice and found that sub Spermine reduces the levels of circulating i...

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PUM

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Abstract

The invention discloses an application of spermidine in preparation of an aortic aneurysm treatment drug. The application has beneficial effects as follows: spermidine can be taken as a drug for delaying progression of abdominal aortic aneurysm, has a good treatment effect in animal experiments, and has clearer toxicology and pharmacology, higher safety and weak toxic and side effects.

Description

technical field [0001] The invention relates to the application of spermidine in the preparation of medicines for treating aortic aneurysms, and belongs to the technical field of medicines for aortic aneurysms. Background technique [0002] An aneurysm is defined as a permanent localized dilation of the arterial wall exceeding 50% of the normal vessel diameter. Abdominal aortic aneurysm is the most common type of aneurysm, most commonly located in the lower segment of the abdominal aorta and renal arteries. Abdominal aortic aneurysm is an age-related arterial degenerative disease, and its occurrence is related to many epidemiological factors, such as age, smoking, gender, race, and family history. With the aging population in our country and concurrent high-risk factors such as hypertension and arteriosclerosis, the incidence of abdominal aortic aneurysm is also increasing. [0003] The clinical manifestations of abdominal aortic aneurysm are progressive and persistent tum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/132A61P9/14
CPCA61K31/132A61P9/14
Inventor 王伟刘帅
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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