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Method for preparing molybdenum disulfide/silver nano-immune substrate material, and repeatable immunodetection application thereof

A molybdenum disulfide and base material technology, applied in the direction of molybdenum sulfide, analytical materials, material excitation analysis, etc., can solve the problems of loose bonding, uneven size of silver nanoparticles, and unfavorable improvement of MoS2 catalytic activity, etc., to achieve cost reduction Effect

Active Publication Date: 2019-12-03
NINGBO UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since cancer markers are macromolecules, they are not easy to be decomposed by catalysis, and silver nanoparticles synthesized by chemical methods are generally inhomogeneous in size, which is different from that of MoS 2 The combination is relatively loose, which is not conducive to improving MoS 2 Catalytic activity to achieve the degradation of macromolecules

Method used

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  • Method for preparing molybdenum disulfide/silver nano-immune substrate material, and repeatable immunodetection application thereof
  • Method for preparing molybdenum disulfide/silver nano-immune substrate material, and repeatable immunodetection application thereof
  • Method for preparing molybdenum disulfide/silver nano-immune substrate material, and repeatable immunodetection application thereof

Examples

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Effect test

Embodiment 1

[0037] A molybdenum disulfide / silver (MoS 2 / Ag) nano-immune base material and its reproducible immunoassay application, including the following steps:

[0038] 1. Preparation of molybdenum disulfide / silver nano-repeatable immune substrate material: Add 0.3629 g of sodium molybdate dihydrate, 0.33809 g of thioacetamide and 4.0294 g of silicotungstic acid hydrate into 25 ml of deionized water in sequence, and stir for 20 Minutes to make it mix evenly, and add aqueous sodium hydroxide solution (concentration: 1 mmol per milliliter) dropwise to the mixed solution to adjust the pH value of the solution to 7.62. Afterwards, the above reaction solution was transferred to a 100 ml hydrothermal reactor, sealed, and reacted at 220° C. for 24 hours. After the reaction kettle was naturally cooled to room temperature, the solid product was obtained by filtration, and the solid product was washed successively with aqueous sodium hydroxide solution (concentration: 1 mmol per ml), absolute ...

Embodiment 2

[0050] A preparation of a molybdenum disulfide / silver nano-immune base material and its repeatable immunoassay application, comprising the following steps:

[0051] 1. Preparation of molybdenum disulfide / silver nano-repeatable immune substrate material: Add 0.7258 g of sodium molybdate dihydrate, 0.67617 g of thioacetamide and 8.0588 g of silicotungstic acid hydrate into 50 ml of deionized water in sequence, and stir for 15 minutes to mix well. Aqueous sodium hydroxide solution (concentration: 1 mmol / ml) was added dropwise to the mixed solution to adjust the pH value of the solution to 7.62. Afterwards, the above reaction solution was transferred to a 100 ml hydrothermal reactor, sealed, and reacted at 200° C. for 26 hours. After the reaction kettle was naturally cooled to room temperature, the solid product was obtained by filtration, and the solid product was washed successively with aqueous sodium hydroxide (concentration: 1 mmol per milliliter), absolute ethanol and deion...

Embodiment 3

[0056] The preparation of a molybdenum disulfide / silver nano-immune base material and its repeatable immunoassay application comprises the following steps:

[0057] 1. Preparation of molybdenum disulfide / silver nano-repeatable immune substrate material: add 1.0887 g of sodium molybdate dihydrate, 1.01427 g of thioacetamide and 12.0882 g of silicotungstic acid hydrate in 75 ml of deionized water, and stir for 25 minutes to mix well. Aqueous sodium hydroxide solution (concentration: 1 mmol / ml) was added dropwise to the mixed solution to adjust the pH value of the solution to 7.62. Afterwards, the above reaction solution was transferred to a 100 ml hydrothermal reaction kettle, sealed, and reacted at 260° C. for 22 hours. After the reaction kettle was naturally cooled to room temperature, the solid product was obtained by filtration, and the solid product was washed successively with aqueous sodium hydroxide solution (concentration: 1 mmol per milliliter), absolute ethanol and d...

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Abstract

The invention discloses a method for preparing a molybdenum disulfide / silver nano-immune substrate material, and a repeatable immunodetection application thereof. The method is characterized by comprising the following steps: 1) preparing a molybdenum disulfide nanomaterial; 2) spin coating a molybdenum disulfide nano-material solution on a clean silicon wafer, and after vacuum drying, sputteringa layer of Ag nanoparticles on the surface of the silicon wafer by using a magnetron sputtering method to obtain a molybdenum disulfide / silver nanomaterial; and 3) connecting an antibody to the molybdenum disulfide / silver nanomaterial to obtain the molybdenum disulfide / silver nano-immune substrate material. The immunodetection method of the molybdenum disulfide / silver nano-immune substrate material is as follows: dripping an antigen to be tested onto the molybdenum disulfide / silver nano-immune substrate material for incubation, dripping a gold nanorod immune probe solution and performing spectral measurement. After catalytic degradation, the molybdenum disulfide / silver nanomaterial can achieve the repeatable immunodetection of cancer markers, and has the advantages of being low in detection limit and being recyclable.

Description

technical field [0001] The invention relates to the fields of material engineering and nanotechnology, in particular to a molybdenum disulfide / silver (MoS 2 / Ag) nano-immune base material and its reproducible immunoassay application. Background technique [0002] Due to the characteristics of long incubation period, late detection and easy death, cancer has seriously endangered human health and sustainable development of society. Early detection and early treatment have become the key to cancer diagnosis and treatment. Therefore, scientific and medical workers have focused on the development and optimization of early cancer screening technology. The surface-enhanced Raman scattering technology based on noble metal nanomaterials has received great attention due to its ultra-high sensitivity, stable reproducibility and simple operation method. A new immunoassay technique based on surface-enhanced Raman scattering technology has quietly emerged in recent years. Due to the ab...

Claims

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Application Information

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IPC IPC(8): G01N21/65C23C14/35C23C14/16C01G39/06
CPCC01G39/06C01P2004/03C23C14/165C23C14/35G01N21/658
Inventor 牛志强姜涛庄文婷陈颖顾辰杰姜勇王福艳
Owner NINGBO UNIV
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