Cordyceps militaris-mung bean bidirectional liquid fermentation process
A technology of liquid fermentation and Cordyceps militaris, which is applied in cosmetics, skin care preparations, medical preparations containing active ingredients, etc., can solve the problem of less application of cosmetics, improve antioxidant and anti-inflammatory activities, and improve unpleasant odors Effect
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Embodiment 1
[0040] A kind of Cordyceps militaris-mung bean two-way liquid fermentation process, comprises the following steps:
[0041] S1, configure liquid culture medium
[0042] The mung bean mixture with a solid-liquid ratio of 1:30 was extracted at 70°C for 1.5h, filtered, and the filtrate was obtained to obtain a mung bean extract, and then 60g of glucose, 4.5g of MgSO4, and 1.5g of KH2PO4 were weighed and mixed evenly, and then Add 3L mung bean extract to dissolve, add an appropriate amount of defoamer, assist ultrasound, stir for 3 minutes to promote the dissolution of the medium, and obtain a liquid medium.
[0043] S2, fermentation culture
[0044] Sterilize the cleaned 5L fermenter, test the air tightness of the equipment at the same time, and calibrate the pH and DO (dissolved oxygen) electrodes. After the calibration, transfer the liquid medium to the fermenter, insert the electrodes, and remove them. Wrap the lead wire and the electrode head with tinfoil paper, connect the...
Embodiment 2
[0048] A kind of Cordyceps militaris-mung bean two-way liquid fermentation process, comprises the following steps:
[0049]S1, configure liquid culture medium
[0050] Extract the mung bean mixture with a solid-liquid ratio of 1:10 at 50°C for 1 hour, filter, and take the filtrate to obtain the mung bean extract, then weigh 40 g of glucose, 4 g of MgSO4, and 1 g of KH2PO4 and mix well, then add 2 L of mung bean The extract was dissolved, an appropriate amount of antifoaming agent was added, assisted by ultrasound, and stirred for 3 minutes to promote the dissolution of the medium to obtain a liquid medium.
[0051] S2, fermentation culture
[0052] Sterilize the cleaned 5L fermenter, test the air tightness of the equipment, and calibrate the pH and DO (dissolved oxygen) electrodes. After the calibration, transfer the liquid culture medium to the fermenter, insert the electrodes, and remove them. Wrap the lead wire and the electrode head with tinfoil paper, connect the pipeli...
Embodiment 3
[0056] A kind of Cordyceps militaris-mung bean two-way liquid fermentation process, comprises the following steps:
[0057] S1, configure liquid culture medium
[0058] Extract the mung bean mixture with a solid-liquid ratio of 1:20 at 60°C for 2 hours, filter, and take the filtrate to obtain the mung bean extract, then weigh 50g of glucose, 5g of MgSO4, 2g of KH2PO4 and mix them evenly, then add 2.5L The mung bean extract was dissolved, and an appropriate amount of defoamer was added, assisted by ultrasound, and stirred for 3 minutes to promote the dissolution of the medium to obtain a liquid medium.
[0059] S2, fermentation culture
[0060] Sterilize the cleaned 5L fermenter, test the air tightness of the equipment, and calibrate the pH and DO (dissolved oxygen) electrodes. After the calibration, transfer the liquid culture medium to the fermenter, insert the electrodes, and remove them. Wrap the lead wire and the electrode head with tinfoil paper, connect the pipeline, w...
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