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Ratio type fluorescent molecularly imprinted paper chip, preparation method and application thereof

A fluorescent molecular imprinting, paper chip technology, applied in fluorescence/phosphorescence, material analysis by optical means, analysis of materials, etc., can solve the problems of measurement value and true value error, poor selectivity, etc. The effect of detection

Active Publication Date: 2019-11-26
陕西国环绿城环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although carbon quantum dots have good fluorescence characteristics, as far as carbon quantum dots are concerned, as a fluorescent probe, its biggest disadvantage is poor selectivity.
In addition, fluorescent probes in the prior art are generally only detected according to a change in the fluorescent signal, which easily leads to the problem of errors between the measured value and the real value

Method used

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  • Ratio type fluorescent molecularly imprinted paper chip, preparation method and application thereof
  • Ratio type fluorescent molecularly imprinted paper chip, preparation method and application thereof
  • Ratio type fluorescent molecularly imprinted paper chip, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for preparing a ratiometric fluorescent molecularly imprinted paper chip, such as figure 1 shown, including the following steps:

[0036] a. Synthesis of CDs: Weigh 0.15g of folic acid powder and add it into 50mL of secondary water, stir well, then transfer the solution to a reaction kettle at 180°C for 2 hours, and cool naturally after the reaction is completed. The synthesized CDs were passed through a 0.22 μm water-based filter membrane, and then purified through a G-25 Sephadex column. The obtained pure CDs were protected from light with tinfoil and placed in a dark place for later use;

[0037] b. Modification of CDs: Add 7.128 mg of L-cysteine ​​to 18 mL of the CDs solution synthesized in step a, and incubate at 37°C for 40 minutes under dark conditions, then store in the dark for later use;

[0038]c. Synthesis of cellulose fluorescent paper embedded with CDs: First, put a cut cellulose paper of suitable size in a petri dish, add 40mL of 0.2% dilute hyd...

Embodiment 2

[0044] A method for preparing a ratiometric fluorescent molecularly imprinted paper chip, comprising the following steps:

[0045] a. Synthesis of CDs: Weigh 0.15g of folic acid powder and add it into 40mL of secondary water, stir well, then transfer the solution to a reaction kettle at 170°C for 2 hours, and cool naturally after the reaction is completed. The synthesized CDs were passed through a 0.22 μm water-based filter membrane, and then purified through a G-25 Sephadex column. The obtained pure CDs were protected from light with tinfoil and placed in a dark place for later use;

[0046] b. Modification of CDs: Add 7.128 mg of L-cysteine ​​to 18 mL of the CDs solution synthesized in step a, and incubate at 36°C for 35 minutes under dark conditions, then store in the dark for later use;

[0047] c. Synthesis of cellulose fluorescent paper embedded with CDs: First, put a cut cellulose paper of appropriate size in a petri dish, add 35mL of 0.2% dilute hydrochloric acid to ac...

Embodiment 3

[0053] A method for preparing a ratiometric fluorescent molecularly imprinted paper chip, comprising the following steps:

[0054] a. Synthesis of CDs: Weigh 0.15g of folic acid powder and add it into 60mL of secondary water, stir well, then transfer the solution to a reaction kettle at 190°C for 3h, and cool naturally after the reaction is completed. The synthesized CDs were passed through a 0.22 μm water-based filter membrane, and then purified through a G-25 Sephadex column. The obtained pure CDs were protected from light with tinfoil and placed in a dark place for later use;

[0055] b. Modification of CDs: Add 7.128mg of L-cysteine ​​to 20mL of the CDs solution synthesized in step a, incubate at 38°C for 45min under dark conditions, and then store in the dark for later use;

[0056] c. Synthesis of cellulose fluorescent paper embedded with CDs: First, put a cut cellulose paper of appropriate size in a petri dish, add 45mL of 0.2% dilute hydrochloric acid to acidify, shake...

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Abstract

The invention provides a ratio type fluorescent molecularly imprinted paper chip, a preparation method and application thereof and belongs to the technical field of material science and engineering and micro-fluidic chips. The method comprises the following steps that: 1, the surface of cellulose paper is sequentially modified with CDs and an APTES-NBD conjugate fluorescent material; difenoconazole imprinted holes are formed in the surface of the cellulose paper through a surface imprinting technology, so that fluorescent molecularly imprinted paper can be prepared; and the fluorescent molecularly imprinted paper is fixed on a microfluidic substrate of a three-dimensional structure, so that the ratio type fluorescent molecularly imprinted paper chip can be obtained. The microfluidic rapiddetection paper chip of the present invention can realize the rapid detection of difenoconazole and has the advantages of low toxicity, portability, high efficiency, economical efficiency and the like.

Description

technical field [0001] The invention belongs to the technical fields of material science engineering and microfluidic chips, and in particular relates to a ratio-type fluorescent molecular imprinted paper chip and its preparation method and application. Background technique [0002] Carbon quantum dots (CDs) are a kind of carbon-based zero-dimensional materials, nano-clusters composed of very few molecules or atoms, approximately spherical and diameter <10nm, the structure and composition of carbon quantum dots determine the diversity of their properties, Carbon quantum dots have strong absorption peaks in the ultraviolet region and long tails in the visible region. Most of the absorption peaks are concentrated at 260-320nm, and usually exhibit optical characteristics such as fluorescence maximum emission wavelength and excitation wavelength dependence; Carbon quantum dots with good water solubility will emit bright fluorescence under light, and have good optical stabilit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 张忠秦潇潇马昕
Owner 陕西国环绿城环保科技有限公司
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