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Lycium ruthenicum anthocyanin and preparation method thereof

A black wolfberry anthocyanin, drying technology, applied in the direction of organic chemistry, metabolic diseases, endocrine system diseases, etc., can solve the problems of low product extraction rate, toxic and side effects, etc.

Pending Publication Date: 2019-11-26
青海省第四人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gong Fangfang et al. used 70% acidic ethanol as the extraction solution to extract anthocyanins from black wolfberry by ultrasonic-assisted method, but the organic solvent in this method has certain toxic and side effects, and the extraction rate of the product is low

Method used

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  • Lycium ruthenicum anthocyanin and preparation method thereof
  • Lycium ruthenicum anthocyanin and preparation method thereof
  • Lycium ruthenicum anthocyanin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A preparation method of black wolfberry anthocyanins, comprising the following steps:

[0037] Step 1. Preparation of black wolfberry anthocyanin powder: select black wolfberry and remove impurities, dry at 60°C for 12 hours, then pulverize through a high-speed pulverizer, pass through a 120-mesh sieve, prepare black wolfberry anthocyanin powder, pack bag spare;

[0038] Step 2. Black wolfberry anthocyanin extraction: Weigh the black wolfberry anthocyanin dry powder, add ethanol according to the mass volume ratio of 1:10, extract with ultrasonic power 120w for 25 minutes, centrifuge at 8000r / min for 10min, take out The supernatant, and the filter residue was repeatedly extracted, centrifuged, and the combined extract was the black wolfberry anthocyanin extract.

[0039] Step 3, purification and freeze-drying of black wolfberry anthocyanins: Pour ethanol with a volume fraction of 95% into a chromatographic column equipped with a macroporous resin, soak the macroporous r...

Embodiment 2

[0041] A preparation method of black wolfberry anthocyanins, comprising the following steps:

[0042] Step 1. Preparation of black wolfberry anthocyanin powder: select black wolfberry and remove impurities, dry at 55°C for 15 hours, then pulverize through a high-speed pulverizer, and pass through a 100-mesh sieve to prepare black wolfberry anthocyanin powder. bag spare;

[0043] Step 2. Black wolfberry anthocyanin extraction: Weigh the black wolfberry anthocyanin dry powder, add ethanol according to the mass volume ratio of 1:15, extract with ultrasonic power 100w for 30 minutes, centrifuge at 5000r / min for 15min, take out The supernatant, and the filter residue was repeatedly extracted, centrifuged, and the combined extract was the black wolfberry anthocyanin extract.

[0044]Step 3. Purification and freeze-drying of black wolfberry anthocyanins: Pour ethanol with a mass fraction of 95% into a chromatographic column equipped with a macroporous resin, soak the macroporous res...

Embodiment 3

[0046] A preparation method of black wolfberry anthocyanins, comprising the following steps:

[0047] Step 1. Preparation of black wolfberry anthocyanin powder: select black wolfberry and remove impurities, dry at 65°C for 10 hours, then pulverize through a high-speed pulverizer, and pass through a 150-mesh sieve to prepare black wolfberry anthocyanin powder. bag spare;

[0048] Step 2. Black wolfberry anthocyanin extraction: Weigh the black wolfberry anthocyanin dry powder, add ethanol according to the mass volume ratio of 1:5, extract with ultrasonic power 150w for 20 minutes, centrifuge at 10000r / min for 5min, take out The supernatant, and the filter residue was repeatedly extracted, centrifuged, and the combined extract was the black wolfberry anthocyanin extract.

[0049] Step 3. Purification and freeze-drying of black wolfberry anthocyanins: Pour ethanol with a mass fraction of 95% into a chromatographic column equipped with a macroporous resin, soak the macroporous res...

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PUM

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Abstract

The invention discloses a Lycium ruthenicum anthocyanin and a preparation method thereof. The preparation method comprises the following steps: preparing a Lycium ruthenicum anthocyanin powder; extracting the Lycium ruthenicum anthocyanin; and purifying and freeze-drying the Lycium ruthenicum anthocyanin. The Lycium ruthenicum anthocyanin prepared in the invention has good ability to remove DPPH free radicals and hydroxyl free radicals, and has influences on fat accumulation, lipid metabolism and insulin tolerance of obese mice.

Description

technical field [0001] The invention belongs to the technical field of natural product development, and in particular relates to a black wolfberry anthocyanin and a preparation method thereof. Background technique [0002] Anthocyanidin, also known as anthocyanin, is a kind of water-soluble natural pigment widely present in plants in nature, and belongs to flavonoids. Anthocyanins have the application value of anti-blood lipid, hypoglycemic, anti-oxidation, immune regulation, anti-aging, prevention and treatment of cardiovascular disease, arteriosclerosis, tumor drug. Similar to flavonoids in the molecular mechanism of scavenging free radicals, it can scavenge a variety of oxygen free radicals in vivo and in vitro, and can promote and activate various antioxidant enzymes in the body; the polyphenol structure can complex metal ions to prevent such Metal ions with redox activity catalyze to accelerate the generation of free radicals, and finally enhance their antioxidant effe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62A61P39/06A61P3/04A61P3/06A61P5/50
CPCC07D311/62A61P39/06A61P3/04A61P3/06A61P5/50
Inventor 莫晨玲李长兴谈燕燕童广清王爱超孙超
Owner 青海省第四人民医院
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