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Traditional Chinese medicine grub glucan and application thereof

A technology of glucan and glucan sulfate is applied in the field of preparing aldolase A inhibitor medicine, traditional Chinese medicine grub glucan or its sulfated derivatives, and can solve the problems such as application reports of grub polysaccharide not found. , to achieve the effect of good inhibitory activity, strong specific affinity and enhanced activity

Active Publication Date: 2019-11-22
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polysaccharide is a component of grub. According to the inventor's data retrieval, no report on the application of grub polysaccharide as an ALDOA inhibitor has been found so far.

Method used

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  • Traditional Chinese medicine grub glucan and application thereof
  • Traditional Chinese medicine grub glucan and application thereof
  • Traditional Chinese medicine grub glucan and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: Preparation of traditional Chinese medicine grub dextran

[0069] Weigh 5kg of dried grub medicinal material, pulverize it into a coarse powder; reflux extraction with 15000mL ethanol for 2h, filter out the ethanol extract and discard it, treat the grub drug residue once more according to the above method, filter out the ethanol extract and discard it, the residue Evaporate ethanol to dryness, after drying at 50°C, reflux extraction with 25000mL distilled water at 100°C, extract 3 times in total, 3h each time. The combined water extracts were concentrated to 10000mL under reduced pressure with a rotary evaporator at 60°C; using the Sevag method (i.e., V polysaccharide solution: V chloroform: V n-butanol = 20:4:1, mixed for 30 min, centrifuged, allowed to stand to separate layers, and the water layer was taken ) to remove protein, repeated 5 times, and centrifuged to discard the precipitate. Decolorize with 30% aqueous hydrogen peroxide solution with 80 times...

Embodiment 2

[0074] Example 2: Preparation of traditional Chinese medicine grub dextran

[0075] Weigh 1 kg of dried grub medicinal material, crush it into medium powder; reflux extraction with 4000mL ethanol for 3 hours, filter out the ethanol extract and discard it, reprocess the grub drug residue once more according to the above method, filter off the ethanol extract, and evaporate the drug residue to dryness After drying with ethanol at 50°C, reflux extraction with 8000mL water at 100°C for 2 times, each time for 3h; combine the water extracts, concentrate under reduced pressure on a 65°C rotary evaporator to 800mL, freeze and thaw repeatedly 8 times, centrifuge, and discard the precipitate; Use the Sevag method (i.e. V polysaccharide solution: V chloroform: V n-butanol = 20:4:1 to mix for 10 minutes, centrifuge, let stand to separate layers, take the water layer) to remove protein, repeat 2 times, centrifuge and discard the precipitate; then use the sample 10 times the volume of 30% h...

Embodiment 3

[0080] Example 3: Preparation of traditional Chinese medicine grub dextran

[0081] Weigh 10kg of dried grub medicinal material, crush it into a coarse powder; reflux extract with 40000mL of absolute ethanol for 2h, filter out the ethanol extract and discard it, process the grub drug residue once more according to the above method, filter out the ethanol extract, evaporate the drug residue Dry ethanol, after drying at 40°C, reflux extraction with 60,000mL aqueous solution at 100°C, 3 times, 2h each time, combine the water extracts, concentrate to 10,000mL with a rotary evaporator at 60°C, cool to room temperature and use the Sevag method (i.e. V polysaccharide solution: V chloroform: V n-butanol = 20:4:1 Mix and shake for 30 minutes, centrifuge, let stand to separate layers, take the water layer) to remove protein, repeat 4 times, centrifuge, and discard the precipitate. The aqueous solution was decolorized with 30% hydrogen peroxide aqueous solution 50 times the sample volume...

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Abstract

The invention belongs to the field of traditional Chinese medicine modernization, and relates to traditional Chinese medicine grub glucan and application thereof. The molecular weight of the traditional Chinese medicine grub glucan is 1-4*10<4>Da, the composition of monosaccharide is glucose, and the sugar content is greater than 90%. The traditional Chinese medicine grub glucan has strong specificity affinity with ALDOA and can inhibit the activity of ALDOA, so that the traditional Chinese medicine grub glucan has remarkable effects of preventing or treating cancers, diabetes and complications thereof, obesity and related metabolic diseases thereof, viral meningitis, arthritis and myocardial hypertrophy.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine modernization, and in particular relates to the traditional Chinese medicine grub dextran or its sulfated derivative and its application in the preparation of aldolase A inhibitor medicine. Background technique [0002] Glucose can provide energy to cells in two ways. Under aerobic conditions, it is through mitochondrial oxidative phosphorylation in cells, and under anaerobic conditions, it is in the form of glycolysis. The process of decomposing glucose into pyruvate through glycolysis requires 10 steps of enzyme-catalyzed reactions; more than 30 enzymes participate in the completion. Among them, 1,6-diphosphate-D-fructose aldolase (ALDO) is an important metabolic enzyme of glycolysis. In vertebrates, the ALDO family consists of three isozymes encoded by structurally very similar genes that are expressed in a tissue-dependent manner: ALDOA (mainly in muscle), ALDOB (mainly in liver )...

Claims

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Application Information

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IPC IPC(8): C08B37/02A61K31/716A61P1/16A61P3/04A61P3/10A61P9/00A61P9/10A61P9/12A61P13/12A61P17/00A61P25/00A61P27/02A61P35/00
CPCA61K31/716A61P1/16A61P3/04A61P3/10A61P9/00A61P9/10A61P9/12A61P13/12A61P17/00A61P25/00A61P27/02A61P35/00C08B37/0006
Inventor 曹蔚王晶梅谯雨荷任丽
Owner NORTHWEST A & F UNIV
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