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Medical dressing loaded with nano silver and bioactive factors and preparation method thereof

A bioactive factor and nano-silver technology, applied in medical science, absorbent pads, bandages, etc., can solve the problems of low bioavailability, low bioavailability, easy to be hydrolyzed by enzymes, etc., and achieve stable preparation process and antibacterial performance Good, mild operating conditions

Inactive Publication Date: 2019-11-22
南昌大学第一附属医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Growth factors secreted by industrially prepared stem cells widely exist in human skin cells. The biological activity of these small molecular proteins is greatly affected by different external environments, and they are sensitive to heat and acid, unstable in nature, and easily hydrolyzed by enzymes. Very short half-life, low bioavailability when administered systemically
When used as a functional additive to wound dressings, efficacy is often limited due to low bioavailability

Method used

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  • Medical dressing loaded with nano silver and bioactive factors and preparation method thereof
  • Medical dressing loaded with nano silver and bioactive factors and preparation method thereof
  • Medical dressing loaded with nano silver and bioactive factors and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of nano silver / epidermal growth factor EGF / chitosan / sodium alginate chronic wound dressing:

[0038] a. First chitosan is dissolved in 1.0% acetic acid solution by mass fraction to prepare 1.5% (W / V) chitosan solution, then add 3% CaCl2 (W / V) solution, stir and dissolve to obtain chitosan Calcium chloride mixed solution; prepare 2.5% (W / V) sodium alginate solution, and dissolve the epidermal growth factor EGF in a ratio of 1:1 to obtain a sodium alginate solution containing EGF;

[0039] b. Preparation of stem cell growth factor: use DMEM medium (complete medium) containing 10% fetal bovine serum, at 37 DEG C, 5% CO Under the condition, the stem cells are routinely cultured until the degree of cell fusion reaches about 90%. The cell concentration reaches 1~10×10 6 , absorb the culture solution containing stem cell growth factors, centrifuge, discard the precipitate, and take the supernatant to freeze-dry for later use;

[0040] c. Use a 10ml syringe to ext...

Embodiment 2

[0046] Preparation of nanosilver / epidermal growth factor EGF / carboxymethyl chitosan / sodium alginate chronic wound dressing:

[0047] a. First, carboxymethyl chitosan is dissolved in 1.0% acetic acid solution by mass fraction to prepare 1.5% (W / V) carboxymethyl chitosan solution, then add 3% CaCl2 (W / V) solution, stir Dissolved to obtain a mixed solution of carboxymethyl chitosan and calcium chloride; prepare a 2.5% (W / V) sodium alginate solution, and dissolve the epidermal growth factor EGF in a ratio of 1:1 to obtain sodium alginate containing EGF solution;

[0048] b. Preparation of stem cell growth factor: use DMEM medium (complete medium) containing 10% fetal bovine serum, at 37 DEG C, 5% CO Under the condition, the stem cells are routinely cultured until the degree of cell fusion reaches about 90%. The cell concentration reaches 1~10×10 6 , absorb the culture solution containing stem cell growth factors, centrifuge, discard the precipitate, and take the supernatant to f...

Embodiment 3

[0055] Preparation of nano-silver / epidermal growth factor EGF / carboxypropyl chitosan / sodium alginate chronic wound dressing:

[0056] a. First carboxypropyl chitosan is dissolved in 1.0% acetic acid solution with mass fraction to prepare 1.5% (W / V) carboxypropyl chitosan solution, then add 3% CaCl2 (W / V) solution, stir Dissolved to obtain a mixed solution of carboxypropyl chitosan and calcium chloride; prepare a 2.5% (W / V) sodium alginate solution, and dissolve the epidermal growth factor EGF in a ratio of 1:1 to obtain sodium alginate containing EGF solution;

[0057] b. Preparation of stem cell growth factor: use DMEM medium (complete medium) containing 10% fetal bovine serum, at 37 DEG C, 5% CO Under the condition, the stem cells are routinely cultured until the degree of cell fusion reaches about 90%. The cell concentration reaches 1~10×10 6 , absorb the culture solution containing stem cell growth factors, centrifuge, discard the precipitate, and take the supernatant to...

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Abstract

The invention relates to a preparation method of chronic wound dressing, and in particular, relates to a medical dressing loaded with nano silver and bioactive factors and a preparation method thereof. The dressing includes calcium alginate, chitosan or a chitosan derivative as a matrix and is loaded with nano silver antibacterial composition particles and a growth factor EGF. During preparation,calcium alginate core microspheres wrapped with the growth factor are obtained by dropwise adding a sodium alginate solution mixed with the growth factor into a calcium chloride solution. The microspheres are added to a chitosan acetic acid solution to form a polyelectrolyte complex. Then the polyelectrolyte complex is mixed and stirred with nano silver and chitosan, the mixture is added to sodiumalginate, crosslinking and freeze-drying are performed, and finally, the chronic wound dressing loaded with nano silver and the growth factor is formed. The raw materials of the method are cheap andeasy to obtain, the preparation process is stable, the bacteriostatic performance of the chronic wound dressing is good, and the wound repair ability is excellent. The dressing is used for treating various chronic wounds caused by cancer radiotherapy, diabetes, ischemia and the like, and induces cleaning and repairing of the wounds.

Description

technical field [0001] The invention belongs to the field of medical dressings, and relates to a medical dressing applied to cleaning and repairing chronic wounds, in particular to a chronic wound dressing loaded with nano-silver and bioactive growth factors and a preparation method thereof. Background technique [0002] The skin is divided into two layers, the epidermis and the dermis. The epidermis is on the surface of the skin, and can be divided into two parts, the stratum corneum and the germinal layer. The epidermis is a stratified squamous epithelium, and the dermis is a dense connective tissue with many elastic fibers and collagen fibers, which are elastic and tough. The dermis is thicker than the epidermis and is rich in blood vessels and nerves. There is subcutaneous tissue under the skin, which is loose connective tissue and has a large number of fat cells. The skin can protect internal tissues from external damage; carry out metabolism such as absorption, persp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/44A61L15/46
CPCA61L15/28A61L15/44A61L15/46A61L2300/104A61L2300/252A61L2300/404A61L2300/414A61L2300/45C08L5/04C08L5/08
Inventor 刘洋许恒毅黄瑾宛林溪
Owner 南昌大学第一附属医院
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