Phnom Penh rose active extract, skin care compound, preparation and application of extract and skin care product
A Phnom Penh rose, extract technology, applied in the field of skin care products, can solve the problems of safety and allergies
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[0047] In addition, the above-mentioned preparation method of the active extract of Rose Phnom Penh comprises the following steps: after crushing the dried rose flower of Phnom Penh Rose, cold leaching with ethanol aqueous solution with a volume fraction of 65%-95% for several times, combining the filtrates to obtain the extract, The extract was concentrated under reduced pressure to obtain ethanol extract. Add water to the ethanol extract to suspend, extract with petroleum ether for several times, and concentrate under reduced pressure to obtain petroleum ether extract and ethanol extract. The ethanol extract is dissolved in water, and the active ingredient is enriched by macroporous adsorption resin (HP-20). First, the volume fraction is 0-30% methanol aqueous solution to elute for 4-10 column volumes to remove the large polar component, and then use volume fraction The methanol aqueous solution with a fraction of 50%-70% is eluted in 8-10 column volumes, and the eluate obta...
Embodiment 1
[0059] The present embodiment mainly extracts a kind of Phnom Penh Rose extract from Phnom Penh Rose, specifically comprises the following steps:
[0060] After 800g of Phnom Penh rose dried flower is pulverized, it is 95% ethanol aqueous solution cold leaching extraction three times with volume fraction, and the filtrate after merging is concentrated under reduced pressure and obtains 95% ethanol extract; Remaining plant residue is 65% ethanol aqueous solution cooling After leaching three times, the combined filtrate was concentrated under reduced pressure to obtain a 65% ethanol extract. Add water to the 95% ethanol extract to suspend, extract three times with petroleum ether, and concentrate under reduced pressure to obtain petroleum ether extract (7.431 g) and the aqueous phase part of the 95% ethanol extract. The aqueous phase part of the 95% ethanol extract and the 65% ethanol extract were combined, and freeze-dried to obtain the ethanol aqueous crude extract of Rose Phn...
Embodiment 2
[0064] This embodiment mainly isolates the skin care compound from the active extract of Rose Phnom Penh prepared in Example 1, and the specific steps are as follows:
[0065] Select 15 g of the active extract of Rose Phnom Penh prepared in Example 1 through silica gel (200 mesh) normal phase silica gel column chromatography, with chloroform-methanol solvent system (chloroform / methanol volume ratio is 9:1, 7:1, 5:1, 3:1, 1:1), and the eluate was combined into 9 fractions (D1-D9) after detection by thin-layer chromatography. Fraction D2 (eluted when the volume ratio of chloroform / methanol was 9:1) was separated by semi-preparative HPLC (YMC-Pack ODS-AQ, 40% methanol-water) to obtain compound 1 (44mg); Fraction D3 (chloroform eluting at a volume ratio of 5:1) to compound 2 (30 mg) was separated by semi-preparative HPLC (YMC-Pack ODS-AQ, 45% methanol-water).
[0066] Compounds 1 and 2 obtained in the above-mentioned separation steps of the present embodiment were subjected to 1...
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