Establishing method of rat atrial fibrillation model for simulating characteristics of human diseases
A model and disease technology, applied in the medical field, can solve problems such as many animal ethics problems, high technical threshold, cardiac pacemaker pacing wires and expensive consumables for pulse generators, etc., and achieve the effect of simple and convenient establishment method
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Embodiment 1
[0031] Embodiment 1 Construction of aortic coarctation model mouse
[0032] 40-week-old SD rats (20 in total) were randomly divided into a sham operation group and a coarctation of the aorta (TAC) group, with 10 rats in each group. The method of cervical aortic arch constriction (TAC) is as follows: rats are anesthetized by intraperitoneal injection of 1% amobarbital, the skin of the neck is depilated, an incision is made through the neck to the level of the manubrium of the rat, and the muscles are separated layer by layer. and fascia, bilateral carotid arteries can be seen after fully exposing the trachea, continue to separate along the left carotid artery and expose the aortic arch, and three branch vessels (brachiocephalic trunk, left common carotid artery, left subclavian artery) can be seen, of which the brachiocephalic artery The portion of the aortic arch between the trunk and the left common carotid artery is the target site for surgical narrowing. The 18G needle was...
Embodiment 2
[0033]Example 2 Detection of atrial remodeling in TAC model mice
[0034] For the TAC model mice obtained in Example 1, continue to feed for 1 week, conduct small animal ultrasound (vevo2100 ultrasound imaging system) detection, measure the blood flow velocity at the constriction of the aortic arch of the rat, continue to raise until after the TAC operation for 8 weeks, detect the inner diameter of the atrium, And the glucose metabolism level of rat atrial myocardium was detected by PET. According to the change of the inner diameter of the atrium and the change of the glucose metabolism level of the atrial muscle, the occurrence of cardiac remodeling was evaluated.
[0035] Ultrasound detection was performed 1 week after TAC, and the two-dimensional ultrasound images of the aortic arch in the coarctation group (TAC group) showed obvious stenosis; color Doppler ultrasound showed a significant increase in blood flow velocity at the stenosis, see figure 1 (The first column and t...
Embodiment 3
[0037] The establishment of embodiment 3 mouse atrial fibrillation model
[0038] It is confirmed by Example 2 that after the atrial enlargement of the TAC model mice occurs, that is, cardiac remodeling, further transesophageal electrical stimulation is performed to induce atrial fibrillation. The specific operation is as follows: the limbs of the rats are connected to the limb lead electrodes of the electrocardiogram, and the electrocardiogram is recorded. Connect the tail end of the 6F bipolar pacing wire to the electric stimulator, insert the head end into the esophagus of the rat through the mouth, the insertion depth is about 6-7cm, and the electric stimulation parameters are: voltage 40-50V, pulse frequency 20Hz, pulse width 4 ms, continuous stimulation for 30 s, and at the same time record the surface electrocardiogram of the experimental mice with a bioelectrical signal recorder.
[0039] Each experimental mouse was repeated 10 times for transesophageal electrical sti...
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