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A method for detecting the content of aroma components in roses

A technology of aroma components and detection methods, which is applied in the field of chemical detection, can solve the problems of high technical requirements for testing personnel, high requirements for testing instruments, and high cost of reference substances, and achieve the effects of improving measurement efficiency, cheap solvents, and accurate and reliable measurement results

Active Publication Date: 2020-09-29
GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The purpose of the present invention is to overcome the defects in the prior art that the reference substance is high in cost, difficult to obtain, high in requirements for testing instruments, long in running time, complicated in processing methods for the test product, and high in the technical requirements of testing personnel, and provides a method that uses one test and multiple evaluations. This method is used to determine the aroma components of roses. The pure substance n-butanol, which does not exist in the sample and is stable and easy to obtain, is used as an internal standard, and citronellol is used as a reference to establish the components and the volatile components of roses. The relative correction factor between the components is calculated by the correction factor to calculate the content of each component in the rose fragrance, and the headspace gas chromatography is used for determination. The specific method is as follows:

Method used

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  • A method for detecting the content of aroma components in roses
  • A method for detecting the content of aroma components in roses
  • A method for detecting the content of aroma components in roses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Preparation of internal standard solution: Accurately weigh 0.2g of n-butanol, add 5-15°C ultrapure water to dissolve and set the volume to 100ml;

[0062] Preparation of the test solution: Take an appropriate amount of rose petals, cut them into 1cm at 5-15°C 2 For the following fragments, weigh 4g of broken rose petals, put them in a 20ml headspace bottle, then use a pipette to take 5ml of 1-2mg / ml n-butanol aqueous solution at 5-15°C, cover and seal, and then you can get the test product solution.

[0063] Preparation of reference substance solution: Accurately weigh 0.03 g of citronellol reference substance, place it in a headspace bottle, add 5 ml of internal standard solution, and seal it with a cap;

[0064] Preparation of mixed reference substance solution: Accurately weigh 0.1-1.5 g of geraniol, 0.05-0.8 g of nerol, 0.15-1.8 g of 2-phenylethyl alcohol, 0.15-1.7 g of eugenol, and 0.2 g of eugenol methyl ether according to the weight gradient. ~2.5g, linalool 0...

Embodiment 2

[0074] Preparation of internal standard solution: Accurately weigh 0.1g of n-butanol, add 5-15°C ultrapure water to dissolve and set the volume to 100ml;

[0075] Preparation of the test solution: Take an appropriate amount of rose petals, cut them into 1cm at 5-15°C 2 For the following fragments, weigh 4.5g of broken rose petals, put them in a 20ml headspace bottle, then use a pipette to take 5ml of 1-2mg / ml n-butanol aqueous solution at 5-15°C, cover and seal, and obtain Test solution.

[0076] Preparation of reference substance solution: Accurately weigh 0.04g of citronellol as reference substance, place it in a headspace bottle, add 5ml of internal standard solution, and seal it with a cap;

[0077] Preparation of mixed reference substance solution: Accurately weigh 0.1-1.5 g of geraniol, 0.05-0.8 g of nerol, 0.15-1.8 g of 2-phenylethyl alcohol, 0.15-1.7 g of eugenol, and 0.2 g of eugenol methyl ether according to the weight gradient. ~2.5g, linalool 0.02~0.5g, citronell...

Embodiment 3

[0087] Preparation of internal standard solution: Accurately weigh 0.1g of n-butanol, add 5-15°C ultrapure water to dissolve and set the volume to 100ml;

[0088] Preparation of the test solution: Take an appropriate amount of rose petals, cut them into 1cm at 5-15°C 2 For the following fragments, weigh 5.0g of broken rose petals, put them in a 20ml headspace bottle, then use a pipette to take 5ml of 1mg / ml n-butanol aqueous solution at 5-15°C, cover and seal, to obtain the test product solution.

[0089] Preparation of reference substance solution: Accurately weigh 0.05 g of citronellol reference substance, place it in a headspace bottle, add 5 ml of internal standard solution, and seal it with a cap;

[0090] Preparation of mixed reference substance solution: Accurately weigh 0.1-1.5 g of geraniol, 0.05-0.8 g of nerol, 0.15-1.8 g of 2-phenylethyl alcohol, 0.15-1.7 g of eugenol, and 0.2 g of eugenol methyl ether according to the weight gradient. ~2.5g, linalool 0.02~0.5g, c...

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Abstract

The invention relates to a method for determining content of fragrance components in a rose. The method comprises the following steps of (1) chromatogram condition and system applicability test; (2) preparation of an internal standard solution and a standard solution; (3) preparation of a tested object solution; and (4) determination. A reference substance solution and a tested object are fed andinjected into a gas chromatograph in a headspace way, and the contents of geraniol, nerol, 2-phenylethyl alcohol, eugenol, methyl eugenol, linalool, citronellol, heptadecane and tricosane are calculated in an internal standard way. Only one component, namely, linalool is determined to realize synchronous determination of other components in the rose, and the determination efficiency is improved; and the headspace gas chromatography method is used for determination, so that operation is convenient, time and labor are saved, and a determining result is accurate and reliable; and thus, quality ofthe rose can be effectively controlled.

Description

technical field [0001] The invention relates to the field of chemical detection, in particular to a method for detecting the content of aroma components in roses. Background technique [0002] Rose is an upright deciduous shrub belonging to the family Rosaceae, belonging to the genus Rosarugosa Thunb. It has large and beautiful flowers, a wide variety, and a strong aroma. Roses are native to China and have a long history of cultivation. It is an important spice plant. The main component of the oil is L-aromatic alcohol, with a content of up to 6 per thousand. It is used for food and cosmetics. The petals can be used to make cake fillings, rose wine, and rose syrup. After drying, they can be used to make tea. Flower buds Used as medicine to treat liver, stomach pain, fullness of chest and abdomen and irregular menstruation. Rose contains volatile oil, and the main components in the oil are citronellol, geraniol, nerol, eugenol, and phenylethyl alcohol. In addition, it stil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 潘卫伍庆李兴朱丽英王娇徐家怡李佳蔚
Owner GUIZHOU MEDICAL UNIV
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