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Quantum dot luminescence encoding microsphere with precisely designable encoding signals

A technology of encoding signal and fluorescent encoding is applied in the field of preparation and application of micro-nano materials, which can solve the problems that have not been seen yet, and the preparation process of encoded microspheres is complicated, so as to reduce technical barriers and preparation costs, and the preparation method is simple and efficient, and widely used. Foreground effect

Pending Publication Date: 2019-11-15
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some researchers control the placement of different luminescent materials in different microspheres, and then compound microspheres of different colors to eliminate energy transfer, which will undoubtedly complicate the preparation process of encoded microspheres
However, there is no report on solving energy transfer by controlling the spectral overlap of luminescent materials.

Method used

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  • Quantum dot luminescence encoding microsphere with precisely designable encoding signals
  • Quantum dot luminescence encoding microsphere with precisely designable encoding signals
  • Quantum dot luminescence encoding microsphere with precisely designable encoding signals

Examples

Experimental program
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Effect test

Embodiment 1

[0036] The quantum dot fluorescent coded microspheres prepared in this embodiment that can be precisely designed for coding signals are CdSe / CdS quadruped quantum dots / PSMA fluorescent coded microspheres, wherein the quantum dots with large Stokes shifts are CdSe / CdS quadruped quantum dots. Point, the polymer is styrene-maleic anhydride copolymer (PSMA), and the preparation method is the SPG film emulsification-emulsion solvent evaporation method, and the specific preparation steps are:

[0037] Step 1, 0.1mg of CdSe / CdS tetrapod quantum dots and 0.25g of styrene-maleic anhydride copolymer (PSMA) were dissolved in 4mL of toluene to form a dispersed phase;

[0038] Step 2, the sodium dodecyl sulfate (SDS) of 1g is dissolved in the ultrapure water of 200mL, forms continuous phase;

[0039] Step 3, using an SPG membrane with a pore size of 5 μm, under the nitrogen pressure of 2KPa and the shear force of the continuous phase, a uniform and stable oil-in-water emulsion is prepared;...

Embodiment 2

[0044] The quantum dot fluorescent coded microspheres prepared in this embodiment that can be precisely designed for coding signals are Mn:ZnSe / ZnS quantum dots / PSMA fluorescent coded microspheres, wherein the quantum dots with a large Stokes shift are Mn:ZnSe / ZnS quantum dots Point, the polymer is styrene-maleic anhydride copolymer (PSMA), and the preparation method is the SPG film emulsification-emulsion solvent evaporation method, and the specific preparation steps are:

[0045] Step 1, 10 mg of Mn: ZnSe / ZnS quantum dots and 0.5 g of styrene-maleic anhydride copolymer (PSMA) are dissolved in 8 mL of toluene to form a dispersed phase;

[0046] Step 2, the sodium dodecyl sulfate (SDS) of 1g is dissolved in the ultrapure water of 200mL, forms continuous phase;

[0047] Step 3, using an SPG membrane with a pore size of 5 μm, under the nitrogen pressure of 2KPa and the shear force of the continuous phase, a uniform and stable oil-in-water emulsion is prepared;

[0048] Step 4, ...

Embodiment 3

[0051] The quantum dot fluorescent coded microspheres prepared in this embodiment that can be precisely designed for coding signals are Mn:ZnSe / ZnS quantum dots / PSMA fluorescent coded microspheres, wherein the quantum dots with a large Stokes shift are Mn:ZnSe / ZnS quantum dots Point, the polymer is styrene-maleic anhydride copolymer (PSMA), and the preparation method is a microfluidic method, and the specific preparation steps are:

[0052] Step 1, 5mg of Mn:ZnSe / ZnS quantum dots and 0.25g of styrene-maleic anhydride copolymer (PSMA) were dissolved in 4mL of chloroform to form a dispersed phase;

[0053] Step 2, the sodium dodecyl sulfate (SDS) of 1g is dissolved in the ultrapure water of 200mL, forms continuous phase;

[0054] Step 3, using a small droplet microfluidic device with a pore diameter of 5 μm, wherein the flow rate of the continuous phase is 12 nL / min, the flow rate of the dispersed phase is 8 nL / min, and the continuous phase and the dispersed phase are at T Conv...

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Abstract

The invention discloses a quantum dot luminescence encoding microsphere with precisely designable encoding signals. The microsphere comprises a polymer and quantum dots with a large Stokes shift, which is greater than 50 nm. The emission wavelength of the quantum dots is 450 to 750 nm. The invention also discloses an application of the microsphere in detection of one or more target substances. Thespectrums of quantum dots with different emission wavelengths will not overlap with each other, thus energy transfer in multi-color encoding is avoided; the quantum dot luminescence encoding microsphere, which has precisely designable encoding signals, a strong encoding performance, and a large encoding number, is prepared; solved are the problems that the encoding signals cannot be predicted dueto energy transfer in multi-color encoding, the encoding signals cannot be precisely designed, and the encoding number is limited; the preparation of the encoding microsphere is not strongly dependent on experience anymore; the technology barrier and preparation cost of encoded library construction are largely reduced; and the microsphere has a very important meaning and wide application prospectin the field of liquid biochip detection.

Description

technical field [0001] The invention relates to the field of preparation and application of micro-nano materials, in particular to a quantum dot fluorescent coding microsphere whose coding signal can be precisely designed. Background technique [0002] The liquid phase chip is also called the suspension biochip. Its basic detection principle is to combine microspheres with flow cytometry, and use suspension microspheres as the reaction carrier for liquid phase detection. According to the interaction rules of protein-protein, nucleic acid-nucleic acid, etc. , with high-throughput, rapid detection flow cytometry as the analysis method. In the flow chamber of the flow cytometer, the microspheres are irradiated with laser light. On the one hand, the laser light is used to excite the fluorescence of the microspheres to identify the type of microspheres, and then determine the type of analyte; The fluorescent reporter molecule bound to the substance is detected, and the analyte i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/88B82Y20/00B82Y30/00B01J13/12G01N21/64
CPCC09K11/025C09K11/883B82Y20/00B82Y30/00B01J13/125G01N21/6428G01N21/6486
Inventor 李万万武卫杰
Owner SHANGHAI JIAO TONG UNIV
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