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Method for extracting and preparing pachymic acid A and pachymic acid B from Poria Cocos peels

A technology of pachymic acid and poria cocos bark, which is applied in the field of extraction and preparation of pachymic acid A and pachymic acid B, can solve the problems of sample loss and complex process, and achieve the effects of low cost, good repeatability and high efficiency

Active Publication Date: 2019-11-12
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the above defects or improvement needs of the prior art, the invention provides a method for extracting and preparing pachymic acid A and pachymic acid B from Poria cocos bark, the purpose of which is to provide A method for extracting pachymic acid A and pachymic acid B directly from poria cocos bark with high purity and no loss at the same time, thereby solving the problem of complicated process and sample loss in the existing method for obtaining pachymic acid A and pachymic acid B from poria cocos triterpenes technical problem

Method used

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  • Method for extracting and preparing pachymic acid A and pachymic acid B from Poria Cocos peels
  • Method for extracting and preparing pachymic acid A and pachymic acid B from Poria Cocos peels
  • Method for extracting and preparing pachymic acid A and pachymic acid B from Poria Cocos peels

Examples

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Effect test

Embodiment 1

[0056] A method for extracting and preparing pachymic acid A and pachymic acid B from Poria cocos bark, the steps are as follows:

[0057] (1) Wash, dry and pulverize Poria cocos skin to obtain Poria cocos skin powder, add n-butanol-water two-phase solvent to the poria cocos skin powder according to the ratio of material to liquid 1:10 (w / v), and extract under reflux at 40°C for 0.5h , filter and remove filter residue to obtain filtrate, separate liquid to get n-butanol phase; in the n-butanol-water two-phase solvent, the volume ratio of n-butanol and water is between 0.3:1;

[0058] (2) concentrating the n-butanol phase obtained in step (1) by rotary evaporation under reduced pressure at 40° C. to obtain the extract obtained by leaching and concentrating the Poria Cortex with n-butanol;

[0059] (3) adding ethyl acetate to the extract obtained by extracting and concentrating the Poria cocos obtained in step (2), extracting twice, and merging the ethyl acetate extract;

[006...

Embodiment 2

[0070] A method for extracting and preparing pachymic acid A and pachymic acid B from Poria cocos bark, the steps are as follows:

[0071] (1) Wash, dry and pulverize Poria cocos skin to obtain Poria cocos skin powder, add n-butanol-water two-phase solvent to the poria cocos skin powder at a material-to-liquid ratio of 1:30 (w / v), and extract under reflux at 60°C for 2 hours, Filter and remove the filter residue to obtain the filtrate, and separate the liquid to obtain the n-butanol phase; the volume ratio of n-butanol and water in the n-butanol-water two-phase solvent is between 1:1;

[0072] (2) concentrating the n-butanol phase obtained in step (1) by rotary evaporation under reduced pressure at 50° C. to obtain the extract obtained by leaching and concentrating the Poria Cortex with n-butanol;

[0073] (3) adding ethyl acetate to the extract obtained by extracting and concentrating the Poria cocos obtained in step (2), extracting twice, and merging the ethyl acetate extrac...

Embodiment 3

[0084] A method for extracting and preparing pachymic acid A and pachymic acid B from Poria cocos bark, the steps are as follows:

[0085] (1) Wash, dry and pulverize Poria cocos skin to obtain Poria cocos skin powder, add n-butanol-water two-phase solvent to the poria cocos skin powder at a solid-liquid ratio of 1:50 (w / v), and extract under reflux at 80°C for 3 hours, Filter and remove the filter residue to obtain the filtrate, and separate the liquid to obtain the n-butanol phase; the volume ratio of n-butanol and water in the n-butanol-water two-phase solvent is between 3:1;

[0086] (2) concentrating the n-butanol phase obtained in step (1) by rotary evaporation under reduced pressure at 60° C. to obtain the extract obtained by leaching and concentrating the Poria Cortex with n-butanol;

[0087] (3) adding ethyl acetate to the extract obtained by extracting and concentrating the Poria cocos obtained in step (2), extracting twice, and merging the ethyl acetate extract;

...

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Abstract

The invention discloses a method for extracting and preparing pachymic acid A and pachymic acid B from Poria Cocos peels. The method is characterized in that high-speed countercurrent chromatography is adopted to separate ethyl acetate extract powder of the Poria Cocos peels, wherein the stationary phase is the upper phase of an n-hexane / ethyl acetate / methanol / water mixture; and the mobile phase is the lower phase of the n-hexane / ethyl acetate / methanol / water mixture. The method for simultaneously extracting high-purity pachymic acid A and pachymic acid B from Poria Cocos peels is provided, high-speed countercurrent chromatography is adopted for crude extract of Poria Cocos peels, a solvent system is designed to effectively separate the pachymic acid A and pachymic acid B from total triterpenoids of Poria cocos respectively, and the solvent system is rapidly layered, and makes high-purity pachymic acid A and pachymic acid B separated and purified from the extract within 2 h.

Description

technical field [0001] The invention belongs to the field of natural product extraction, and more specifically relates to a method for extracting and preparing pachymic acid A and pachymic acid B from poria cocos bark. Background technique [0002] Poria cocos (Schw.) Wolf, a fungus of the Polyporaceae family, has a long history of medicinal and edible use in my country. It is a traditional resource for both medicine and food, and is widely used in Chinese medicine clinics, Chinese patent medicines, and health food raw materials. my country is rich in resources of Poria cocos, but the intensive processing of Poria cocos is still lagging behind. As a by-product of Poria cocos processing, the comprehensive utilization rate of Poria cocos skin is lower, resulting in the waste of a lot of resources. [0003] Poria cocos skin is the dry outer skin of Poria cocos sclerotium. It is recorded in "Chinese Pharmacopoeia" that it is sweet, light, flat, and belongs to the lung, spleen, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/42C07C51/47C07C59/46
CPCC07C51/42C07C51/47C07C2603/14C07C59/46
Inventor 黄文殷梦舟刘莹张莉佳王益
Owner HUAZHONG AGRI UNIV
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