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In situ drug delivery system constructed by pachymic acid A and preparation method and application of in situ drug delivery system

A technology of pachymic acid and drugs, which is applied in the field of in-situ drug delivery system constructed by pachymic acid A and its preparation, can solve the problems of less application development and research, and achieve the effects of strong cytotoxicity, diffusion prevention, and stable gel structure

Pending Publication Date: 2019-11-08
哈尔滨贝科德糖生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on natural product gelling factors focuses more on the discovery of new types of NPGs, but less research on its application and development. Based on the advantages of NPG in the field of biology, we are prompted to further develop it as a drug delivery system with good biological activity.

Method used

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  • In situ drug delivery system constructed by pachymic acid A and preparation method and application of in situ drug delivery system
  • In situ drug delivery system constructed by pachymic acid A and preparation method and application of in situ drug delivery system
  • In situ drug delivery system constructed by pachymic acid A and preparation method and application of in situ drug delivery system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Preparation of PAA-DOX Gel drug-loaded gel

[0036] Use an analytical balance to weigh 10 mg of pachymic acid A into a 2 mL liquid phase vial, then add 1 mL of ethanol into the vial for 30 seconds of ultrasonication until completely dissolved, then add 700 μL of 4 mg / mL doxorubicin hydrochloride aqueous solution, and vortex Stable PAA-DOX Gel drug-loaded gel can be formed after mixing for 10 seconds and standing for 10 minutes.

Embodiment 2

[0037] Example 2 Preparation of PAA-DOX Gel drug-loaded gel

[0038] Use an analytical balance to weigh 10 mg of pachymic acid A into a 2 mL liquid phase vial, then add 1 mL of ethanol into the vial for 20 seconds of ultrasonication until it is completely dissolved, then add 700 μL of 4 mg / mL doxorubicin hydrochloride aqueous solution, and vortex Stable PAA-DOX Gel drug-loaded gel can be formed after mixing for 20 seconds and standing for 10 minutes.

Embodiment 3

[0039] Example 3 Preparation of PAA-DOX Gel drug-loaded gel

[0040] Use an analytical balance to weigh 10 mg of pachymic acid A into a 2 mL liquid phase vial, then add 1 mL of ethanol into the vial for 25 seconds of ultrasonication until completely dissolved, then add 700 μL of 4 mg / mL doxorubicin hydrochloride aqueous solution, and vortex Stable PAA-DOX Gel drug-loaded gel can be formed after mixing for 15 seconds and standing for 10 minutes.

[0041] The following examples are the performance characterization of the above-mentioned drug delivery system and its application method and effect verification in drug delivery. The applied system is the PAA-DOX Gel drug-loaded gel prepared in Example 1:

[0042] Example 2 Characterization of PAA-DOX Gel drug-loaded gel

[0043] 1) Infrared spectroscopy to determine drug DOX loading

[0044] Infrared spectra of PAA-DOX Gel and PAA-Gel, DOX such as figure 1 As shown, in the process of drug gel formation, DOX interacts with PAA-Gel...

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Abstract

The invention discloses an in situ drug delivery system constructed by pachymic acid A and a preparation method and application of the in situ drug delivery system, and relates to drug delivery systems. The preparation method of the in situ drug delivery system comprises the steps that (1) a pachymic acid A solution is prepared as a solution A; (2) a doxorubicin hydrochloride solution is preparedas a solution B; and (3) the solution A is mixed with the solution B uniformly, and after standing, a stable PAA-DOX Gel drug-loaded gel is obtained, that is, the in situ drug delivery system constructed by the pachymic acid A is obtained. The gel prepared by the preparation method has a stable structure, PAA and DOX have a synergistic effect, high cytotoxicity is achieved, cancer cells can be killed even at low concentration, and natural product activity is exerted. Due to a special network structure of the PAA-DOX Gel, DOX can be wrapped in a gap of the gel, the spread of drugs is further prevented, and the drugs can be injected intratumorally to achieve persistent reserve and sustained release.

Description

technical field [0001] The invention relates to a drug delivery system, in particular to an in-situ drug delivery system constructed with pachymic acid A, its preparation method and application. Background technique [0002] Low molecular weight gel (Low molecular weight gel, LMWG) refers to the gel formed by self-assembly of low molecular weight gelatin factor with solvent at low concentration (0.1-10wt%). LWMGs have been widely used in biomedical fields, such as cell culture, optoelectronics, catalysis and sensing, drug delivery, etc. Recently, researchers have become interested in a specific type of LMWG—natural product gels (NPG). NPG is formed by the self-assembly of small molecules and solvents without any chemical modification into fibers under mild conditions, and the fibers are further rearranged into an isotropic matrix. [1] . As a gift of nature, the chemical structure and function of natural products are the result of selection and optimization in the long-ter...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61K9/06A61P35/00A61K31/575
CPCA61K31/575A61K31/704A61K9/06A61K9/0024A61P35/00A61K2300/00
Inventor 杨鑫孙莹智康康
Owner 哈尔滨贝科德糖生物科技有限公司
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