Cell separation system and method thereof

A separation system and cell technology, applied in the field of cell separation, can solve the problems of high cost, high difficulty in implementation, and high cost, and achieve the effects of ensuring separation quality, reducing complexity, and controlling use costs

Active Publication Date: 2019-10-22
武汉原生药谷生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Chinese patent document CN108102914A describes an automatic stem cell culture system, which uses a manipulator to grab and place culture bottles, but the structure of this scheme is very complicated, it is difficult to realize, it is easy to pollute, and even if it is realized, there is a problem of high cost. Difficult to promote
Moreover, only a flow chart is given in this document, and the specific structural diagram of the system is not given, and it is difficult for those skilled in the art to implement
Chinese patent document CN102009002A describes a centrifuge, a fluid-connected container inserted into the centrifuge and a method for obtaining a platelet high-enrichment concentrate from whole blood. This separator is only suitable for the separation of cells in blood, not for It is suitable for the isolation of solid tissue-derived cells, and it is not suitable for the isolation of various stem cells. Therefore, the scope of application is limited
However, the structure is still relatively complex and the technical operation is complicated, which brings about the problem of high cost

Method used

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  • Cell separation system and method thereof
  • Cell separation system and method thereof
  • Cell separation system and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] partial reference figure 1 , a cell separation system comprising:

[0056] Centrifugal device 1, centrifugal device 1 is provided with substantially horizontal centrifugal frame 101, and centrifugal frame 101 is connected with centrifugal motor 105, and drives centrifugal frame 101 to rotate, and centrifugal frame 101 is provided with the tube base 3 that is used to place centrifuge tube 2, so that The liquid in the centrifuge tube 2 is centrifuged; the centrifuge device 1 is provided with a corner positioning device, which is used to stop the tube base 3 at a preset circumferential position;

[0057] At least one liquid adding device, the liquid adding device is provided with a pump for injecting preset liquid into the centrifuge tube 2; for example, adding tissue cell cleaning solution and tissue disinfectant;

[0058] At least one accurate liquid addition device is provided with a screw injection device, a peristaltic pump or a diaphragm pump to achieve accurate liq...

Embodiment 2

[0063] On the basis of Example 1, the preferred scheme can be found in figure 1 and image 3 Among them, a crushing device 4 is also provided above the centrifugal frame 101. In the crushing device 4, the lifting device is connected to the crushing motor 43, and the shaft of the crushing motor 43 is fixedly connected to the cutter bar 45, such as through a drill bit clamping mechanism. , through tapered hole or rectangular hole, hexagonal hole cooperates magnet to connect, be provided with at least one crushing blade 49 on the cutter bar 45, preferably arrange a plurality of crushing blades 49 on the cutter bar 45 in this example, on the cutter bar 45 The outer ring is fixed with a non-rotating crushing sleeve 46 . Preferably, the crushing sleeve 46 is fixedly connected to the housing of the crushing motor 43 , for example snap-fitted. The crushing sleeve 46 is provided with a plurality of through holes 48, including round holes or slotted holes;

[0064] The lifting device...

Embodiment 3

[0080] On the basis of Example 2, the preferred scheme is as figure 1 , 5Among them, a suction and filling device 9 is also provided. The structure of the suction and filling device 9 is: one end of the suction and filling pipe 12 is fixedly connected with the suction lifting mechanism, and the end is located on the centrifuge tube 2 placed on the centrifuge frame 101. When the suction filling pipe 12 is lowered, the bottom is located in the position near the bottom of the tube in the centrifuge tube 2. When the suction filling pipe 12 rises, the bottom of the suction filling pipe 12 is higher than the bottom of the centrifuge tube 2. Nozzle;

[0081] The suction lifting mechanism is a screw nut mechanism driven by a motor, a rack and pinion mechanism, a belt mechanism or a crank slider mechanism, or the suction lifting mechanism is a slider mechanism driven by a linear motor, or the suction lifting mechanism is compressed air driven cylinder;

[0082] The other end of the ...

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Abstract

The invention provides a cell separation system and a method thereof, the system comprises a centrifugal device, wherein a centrifugal frame is arranged in the centrifugal device, the centrifugal frame is connected with a centrifugal motor, the centrifugal frame is driven to rotate, a holder for placing a centrifuge tube is provided at the centrifugal frame to centrifuge the liquid in the centrifuge tube; and the centrifugal device is provided with a corner positioning device; at least one liquid adding device is provided, the liquid adding device is provided with a pump for injecting a presetliquid into the centrifuge tube; least one precise dosing device is provided, the precise dosing device is provided with a screw push device, a peristaltic pump or a diaphragm pump to achieve precisefilling of the liquid into the centrifuge tube; at least one suction device is provided, the suction device is provided with a suction pipe which can be lifted and lowered, the suction pipe is connected with a vacuum device or a suction pump, the suction device extends the suction pipe into the centrifuge tube to a predetermined depth, and the liquid in the centrifuge tube is pumped away. The system is capable of automating the process of cell separation, thereby achieving automated cell separation operation at a low cost, and ensuring separation quality.

Description

technical field [0001] The invention relates to the field of cell separation, in particular to a system and method for tissue-derived cell separation. Background technique [0002] In the field of cell therapy, the representative ones include "tumor cell immunotherapy" represented by CAR-T, and "stem cell therapy". In these treatment methods, the steps of cell separation and culture are required. In the prior art , in the cell isolation process, usually manual operation is used. However, manual operation has disadvantages such as low efficiency, high labor intensity, high probability of infection, high error rate and difficulty in quality assurance. The way of manual operation will affect the final treatment effect. In the field of cell detection, in the process of drug sensitivity test and detection of solid tumor tissue, it is also necessary to separate the solid tumor tissue into cells for culture, and then analyze and inspect the tumor cells. These operations are usua...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12M1/33C12M1/12C12M1/00C12N5/077
CPCC12M47/04C12N5/0667C12N2509/10
Inventor 刘东旭
Owner 武汉原生药谷生物医药科技有限公司
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