Synchrotron radiation X-ray microscopic CT suitable for imaging of living small animal limbs and fixing device

Abstract

The invention discloses a synchrotron radiation X-ray microscopic CT suitable for imaging of living small animal limbs and a fixing device. The fixing device comprises an objective table for an anesthetized small animal to be placed, a column fixed to the objective table and a lead cover which covers the objective table and seals the upper part and periphery of the objective table, a synchrotron radiation light source comprises the fixing device, the objective table of the fixing device is fixed to a rotating sample table of the synchrotron radiation X-ray microscopic CT, and moreover, the axis of a sleeve on the objective table is coaxially arranged with the rotating axis of the rotating sample table. The fixing device of the synchrotron radiation X-ray microscopic CT can be used for integrally and locally fixing the living small animal, and meanwhile, the influence of high-dose radiation on the trunk part outside the joints of the limbs can be avoided; the survival of the living small animal can be protected to the greatest extent, and long-term animal experiments can be conveniently carried out.

Description

technical field [0001] The invention relates to a synchrotron radiation X-ray micro-CT and a fixing device suitable for live imaging of limbs of small animals. Background technique [0002] Sports injuries have the characteristics of high recurrence rate and difficult regeneration and repair. They mainly occur in the bones and joints of the extremities, often accompanied by soft tissue injuries. Imaging evaluation is an important means to study the healing of limb injuries in small animals. At present, commonly used imaging methods include microCT, MRI, etc., but limited by the spatial resolution, it is difficult for conventional methods to achieve three-dimensional imaging at ultra-high resolution. [0003] With the emergence of synchrotron radiation micro-CT (Synchrotron radiation micro-CT, SR-μCT), its high luminous flux, high collimation, and high coherence characteristics can be used for three-dimensional imaging of tissue micromorphological structures, and the resoluti...

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Problems solved by technology

Among the disclosed inventions, there is no method and technical parameters for live imaging of small animal limbs

[0004] Moreover, due to the high radiation dose of SR-μCT, which will affect the survival of small animals, it is often necessary to take the isolated tissue for imaging after the animal is killed, and it is impossible to dynamically observe the real-time healing process in the animal body.

Conventional small animal immobilization devices generally use methods such as suspension or clamps to fix and brake the target area. There is no specific device for immobilizing limbs, and there is no protective device for high radiation doses. Therefore, conventional small animal immobilization devices It is not suitable for live imaging of small animals with synchrotron radiation, and there is no small animal fixation device for live imaging of synchrotron radiation among the disclosed inventions

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