A Quantitative Prediction Method of Pollution Degree of Offshore Water Based on Pollution Indicator Bacteria
A technology for water pollution and prediction methods, which is applied in general water supply conservation, testing water, measuring devices, etc., and can solve the problems of inaccurate data, inaccurate reflection of microbial composition information, and prediction of water pollution in sea areas.
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[0086] From August 15th to 28th, 2013, a total of 82 sampling points in 8 areas were selected from the routine monitoring project of the Marine Environmental Monitoring Center, and surface water samples at a depth of 0.5m were collected; The measured values of pollution at sampling points (Table 1); the other part was subjected to high-throughput sequencing after pretreatment such as filtration, DNA extraction, and PCR amplification, to obtain the number of reads of microbial OTUs in each sample, and convert them into relative abundances. A total of 82 samples from different sea areas were used as samples for screening pollution indicator bacteria and establishing a prediction model. The relative abundance of indicator bacteria screened by random forest was used as an independent variable, and a method for quantitatively evaluating the pollution status of sea areas was established. Implementation process reference figure 1 .
[0087] Table 1 The physical and chemical proper...
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