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Rapid detection test strip for HPV antibody, and preparation method and use method thereof

A technology for testing test paper and test strips, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high technical requirements, long cycle, and difficulty in meeting the detection of vaccination effects of vaccinated populations, and achieve low technical requirements and easy operation. Convenient and easy to measure the effect of batch

Active Publication Date: 2019-10-01
瓦至医学科技(上海)有限公司
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  • Application Information

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Problems solved by technology

At present, the ELISA or competitive radioimmunoassay used for antibody detection has the characteristics of long cycle and high technical requirements, and it is difficult to meet the detection of the vaccination effect of a large number of vaccinated populations. Therefore, it is urgent to develop a method that can detect HPV vaccines in batches. Method of inoculation effect

Method used

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  • Rapid detection test strip for HPV antibody, and preparation method and use method thereof
  • Rapid detection test strip for HPV antibody, and preparation method and use method thereof
  • Rapid detection test strip for HPV antibody, and preparation method and use method thereof

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Embodiment Construction

[0054] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0055] 1. Raw material preparation

[0056] (1) Nitrocellulose: purchased from Shanghai Macklin Biochemical Technology Co., Ltd., the article number is M840602.

[0057] (2) Polymer water-absorbent resin (SAP): purchased from Renqiu Huixiang Chemical Co., Ltd., product number 1131.

[0058] (3) PVC sheet: It is made of PVC particles pressed by an extruder, and its thickness is 1mm±0.2mm.

[0059] (4) Anti-human IgG antibody: goat anti-human IgG antibody purchased from Guangzhou Oubian Biological Products.

[0060] (5) Colloidal gold solution: purchased from Beijing Deke Daojin Technology Co., Ltd., the product number is DK-AU.

[0061] (6) HPV L1 and its antibody:

[0062] The whole gene of HPV16L1 and the whole gene of HPV18L1 were respectively constructed in expression vectors by gene cloning technology, and expressed in Escherichia coli.

[0063] 1. H...

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Abstract

The invention relates to the technical field of HPV antibody detection, in particular to a rapid detection test strip for an HPV antibody, and a preparation method and a use method thereof. The test strip comprises a sample pad, a bonding pad, an analysis membrane, a water absorption pad and a handle, which are arranged in sequence along a chromatographic direction, citric acid-sodium citrate is attached to the surface of the sample pad, anti-human IgG antibody-nanogold particles are arranged on the bonding pad, a detection band and a quality control band are arranged on the analysis membrane,the main component of the detection band is HPV L1, and the main component of the quality control band is a secondary antibody bound with an anti-human IgG antibody. According to the test strip, thecorresponding sample pad, the bonding pad, the analysis membrane and the water absorption pad are prepared in an electrostatic spinning manner, so that it is convenient to measure the inoculation effect of HPV vaccine in batches, the detection efficiency of the inoculation effect of the HPV vaccine is improved, and the test strip has the advantages of simple preparation and convenience for batch production, and is suitable for hospitals, disease control, grass root, health unit detection and household use.

Description

technical field [0001] The invention relates to the technical field of HPV antibody detection, in particular to a test strip for rapid detection of HPV antibody, a preparation method and a use method thereof. Background technique [0002] Cervical cancer is one of the most common clinical gynecological tumors, mainly caused by human papillomavirus (HPV) infection, and its incidence is second only to breast cancer, ranking second among female malignant tumors. In developing countries, cervical cancer is the cancer with the highest death rate among women. [0003] So far, more than 120 kinds of HPV have been reported and found, which are divided into high-risk (carcinogenic) HPV, possible carcinogenic and low-risk HPV according to their carcinogenicity. Among high-risk HPV, HPV16, HPV18, HPV31, and HPV33 It is most closely related to the incidence of cervical cancer, especially HPV16 and HPV18 are the most common. According to reports, the detection rate of these two types o...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/543
CPCG01N33/56983G01N33/54306G01N33/54346
Inventor 陈彬
Owner 瓦至医学科技(上海)有限公司
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