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Silk fibroin/manganese dioxide composite microsphere drug carrier with core-shell structure and preparation method thereof

A technology of silk fibroin and manganese dioxide, which is applied in the direction of non-active components of polymer compounds, drug combinations, and pharmaceutical formulations, can solve problems such as harsh reaction conditions, unstable properties of nanoparticles, and complex synthesis techniques of nanoparticles, and achieve The effect of simple process, excellent singlet oxygen generation ability, and controllable drug release ability

Active Publication Date: 2020-05-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the existing MnO 2 Nanoparticle synthesis technology is complex, reaction conditions are harsh, and pure MnO 2 Due to defects such as unstable properties of nanoparticles, the present invention provides a method for preparing silk protein / manganese dioxide composite microspheres with a core-shell structure by self-assembly using silk protein microspheres as a template

Method used

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  • Silk fibroin/manganese dioxide composite microsphere drug carrier with core-shell structure and preparation method thereof
  • Silk fibroin/manganese dioxide composite microsphere drug carrier with core-shell structure and preparation method thereof
  • Silk fibroin/manganese dioxide composite microsphere drug carrier with core-shell structure and preparation method thereof

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Embodiment 1

[0040] The preparation method of the silk fibroin / manganese dioxide composite microsphere drug carrier of the core-shell structure in this embodiment comprises the following steps in sequence:

[0041] (1) Take an appropriate amount of silkworm cocoon shells, wash and dry them, and then use the existing method to perform degumming treatment. Subsequently, the degummed silk fibers are sequentially subjected to the existing steps of dissolving, dialysis, and concentration to obtain silkworm silk fibroin aqueous solution;

[0042] (2) Adjust the silk fibroin concentration in step (1) to 2mg / mL (the solvent is water, the same as other embodiments), and take 10mL and place it in a reaction vessel. Slowly add 1mL of isopropanol dropwise to the silk protein solution, and keep stirring for 30min to make it evenly mixed.

[0043] (3) The mixed solution in step (2) was placed in a -80°C refrigerator, frozen for 12 hours, and then allowed to thaw naturally at room temperature to obtain ...

Embodiment 2

[0049] The preparation method of the silk fibroin / manganese dioxide composite microsphere drug carrier of the core-shell structure in this embodiment comprises the following steps in sequence:

[0050] (1) Take an appropriate amount of silkworm cocoon shells, wash and dry them, and then perform degumming treatment. Subsequently, the degummed silk fibers are sequentially subjected to steps such as dissolving, dialysis, and concentration to obtain an aqueous solution of silkworm silk fibroin;

[0051] (2) Adjust the silk fibroin concentration in step (1) to 2%, take 5 mL and place it in a reaction vessel, add 2 mL of absolute ethanol and stir evenly.

[0052] (3) The mixed solution in step (2) was placed in a -20°C refrigerator, frozen for 24 hours, and thawed naturally at room temperature to obtain a silk protein suspension. The silk protein suspension was centrifuged, washed and sonicated to obtain silk protein microspheres with a diameter in the range of 200-500 nm, and resu...

Embodiment 3

[0055] Embodiment 3 (in situ drug loading)

[0056] The preparation method of the silk fibroin / manganese dioxide composite microsphere drug carrier of the core-shell structure in this embodiment comprises the following steps in sequence:

[0057] (1) Take an appropriate amount of silkworm cocoon shells, wash and dry them, and then perform degumming treatment. Subsequently, the degummed silk fibers are sequentially subjected to steps such as dissolving, dialysis, and concentration to obtain an aqueous solution of silkworm silk fibroin;

[0058] (2) Adjust the concentration of silk fibroin in step (1) to 2mg / mL, take 10mL and place it in a reaction vessel, add 2mg of doxorubicin hydrochloride (DOX) to the silk fibroin solution, and ultrasonically disperse it to mix with silk Vegetarian protein was mixed evenly, and then 1 mL of isopropanol was slowly added dropwise to the reaction solution, and stirring was continued for 30 min to make it evenly mixed.

[0059] (3) The mixed s...

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Abstract

A preparation method for a silk fibroin / manganese dioxide composite microsphere drug carrier having a core-shell structure, comprising: (1) adding a water-soluble organic solvent to an aqueous solution of silk fibroin, performing freezing and natural thawing, and removing the solvent to obtain silk fibroin nanospheres; and (2) mixing the obtained silk fibroin nanospheres with potassium permanganate and polyallylamine hydrochloride uniformly, performing incubation, and removing the solvent, so as to obtain a silk fibroin / manganese dioxide composite microsphere drug carrier having a core-shell structure in which the inner layer is silk fibroin and the outer layer is manganese dioxide. The process is simple and easy to operate. The obtained silk fibroin / manganese dioxide complex microsphere has a rough surface, combines excellent properties of silk fibroin and manganese dioxide, has a high drug loading rate, and can be released in a controlled manner in response to the tumor microenvironment, degrade hydrogen peroxide in the tumor environment to generate oxygen, and improve the efficiency of chemical and photodynamic therapy for tumor, having a wide application prospect in the fields of tumor therapy and medicine.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a preparation method of a core-shell structure silk fibroin / manganese dioxide composite microsphere drug carrier. Background technique [0002] Sol-like manganese dioxide (MnO 2 ) nanoparticles have become emerging materials in the field of biomedicine due to their specific catalytic properties and good biocompatibility. In the field of tumor treatment, manganese dioxide nanoparticles can specifically react with hydrogen peroxide in the tumor microenvironment, catalyze its degradation to generate oxygen and water, thereby improving the hypoxic environment, down-regulating hypoxia-inducible factors, and weakening the tumor Tissue resistance, in turn, helps photosensitizers convert oxygen into singlet oxygen, while improving the efficiency of chemotherapy and photodynamic therapy of tumors. H rich in the tumor microenvironment + and glutathione (GSH) can degrade M...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/50A61K47/42A61K47/02A61K47/64A61K47/52A61K41/00A61K31/704A61K31/337A61P35/00
CPCA61K9/501A61K9/5052A61K31/337A61K31/704A61K41/0071A61K47/52A61K47/64A61P35/00A61K2300/00
Inventor 杨明英王捷陈玉银陈玉平范鑫
Owner ZHEJIANG UNIV
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