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Method for producing p-hydroxyphenylethanol and use of penicillium chrysogenum

A technology for p-hydroxyphenethyl alcohol and Penicillium chrysogenum, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., to achieve the effect of simple process

Active Publication Date: 2019-09-03
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the production of p-hydroxyphenethyl alcohol by Penicillium chrysogenum

Method used

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  • Method for producing p-hydroxyphenylethanol and use of penicillium chrysogenum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The production method of p-hydroxyphenethyl alcohol comprises the steps:

[0044] (1) Steps in which Penicillium chrysogenum DXY-1 is fermented and cultivated to obtain a fermented liquid

[0045] Inoculate the plate-activated Penicillium chrysogenum DXY-1 (derived from the sediment in the Taiwan Strait) into the culture solution, and culture it with shaking at 28°C and 150rmp for 72 hours to obtain the seed culture solution; then transfer the seed culture solution to the culture medium On this basis, cultured with shaking at 28° C. and 150 rpm for 7 days to obtain a fermentation broth. The inoculum amount of the seed culture solution is 5wt% of the weight of the culture medium.

[0046] (2) The step of extracting the fermented liquid to obtain the extract

[0047] Break the mycelium balls in the fermentation broth, then add ethyl acetate to extract 3 times, combine the supernatants, and use a rotary evaporator to concentrate to obtain the extract; the amount of ethyl...

experiment example

[0051] The structure of the p-hydroxyphenethyl alcohol product obtained in Example 1 was identified.

[0052] The ultraviolet spectrum shows that the characteristic absorption peak of benzene appears at a wavelength of about 275nm, and it is speculated that the compound may contain a benzene ring. The K absorption band formed by the conjugated double bond system appears at a wavelength of 220nm, indicating that the compound contains more double bonds.

[0053] H NMR spectrum ( 1 H NMR) gave 10 hydrogen signals. An active hydrogen signal (δ H 9.11, s, 4-OH), four benzene ring hydrogen signals appear in the aromatic region (δ H 6.99, d, H-4; δ H 6.99, d, H-8; δ H 6.63, d, H-5; δ H 6.63, d, H- 7;), overlapping in pairs and splitting into d peaks, indicating that there may be a 1,4-disubstituted benzene ring structure, and a methylene signal appears in the high field area (δ H 2.60, t, H-2), an oxygen-linked methylene signal (δ H 3.50, q, H-1) and an active hydrogen ...

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Abstract

The invention discloses a use of penicillium chrysogenum DXY-1 in the production of p-hydroxyphenylethanol. The invention also discloses a method for producing p-hydroxyphenylethanol, comprising the step of using penicillium chrysogenum DXY-1. The invention adopts the production of p-hydroxyphenylethanol by penicillium chrysogenum for the first time, the process is simple, and the use has great significance for expanding the industrial production of p-hydroxyphenylethanol.

Description

technical field [0001] The present invention relates to a kind of purposes of Penicillium chrysogenum DXY-1, also relate to a kind of production method of p-hydroxyphenyl alcohol. Background technique [0002] p-Hydroxyphenylethanol, also known as 4-hydroxyphenylethanol, commonly known as Tyrosol, is mainly used to synthesize the cardiovascular drug Metoprolol. P-Hydroxyphenethyl alcohol can be synthesized by chemical methods. Use hydroxyacetophenone as a raw material to first synthesize p-hydroxyphenylglyoxal, and obtain p-hydroxyphenethyl alcohol through reduction. Use β-aminophenethyl alcohol as raw material to obtain p-hydroxyphenethyl alcohol through oxidation. [0003] In addition, p-hydroxyphenylethanol can also be obtained by microbial fermentation. For example, CN109370967A discloses the application of a kind of engineering bacteria in the production of tyrosol, amino acid transaminase, glutamate dehydrogenase, ketoacid decarboxylase and alcohol dehydrogenase are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/22C07C37/70C07C37/72C07C37/82C07C39/11C12R1/82
CPCC12P7/22C07C37/685C07C37/70C07C37/72C07C37/82C07C39/11
Inventor 朱虎常爱平王颖璐陈雯丹何侨妹李丽鱼晓丹戴晓芸
Owner FUJIAN NORMAL UNIV
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