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Preparation method of label-free electrochemical sensor for cardiac troponin I and detection method for CTNI

A cardiac troponin and label-free technology, applied in the field of biomedical detection, can solve the problems of high detection limit, low concentration, narrow detection range, etc., and achieve the effect of sensitive detection

Active Publication Date: 2022-04-08
MINNAN NORMAL UNIV
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Problems solved by technology

[0008] However, the detection limit of the above literature is relatively high, and the detection range is narrow. With the continuous improvement of the sensitivity of detecting troponin, the concentration of cTnI in the serum used to evaluate ACS and AMI is continuously decreasing, and is currently at 0.01-0.1ng / In the mL range

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  • Preparation method of label-free electrochemical sensor for cardiac troponin I and detection method for CTNI
  • Preparation method of label-free electrochemical sensor for cardiac troponin I and detection method for CTNI
  • Preparation method of label-free electrochemical sensor for cardiac troponin I and detection method for CTNI

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Embodiment Construction

[0028] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0029] The label-free electrochemical sensor preparation method of cardiac troponin I, the specific steps are as follows:

[0030] Step 1: Put 1mg NH 2 -rGO was dispersed in 1 mL of ultrapure water and sonicated for 10 min to obtain uniform black NH 2 -rGO dispersion;

[0031] Step 2: Preprocess the bare glassy carbon electrode: first, use 1.0μm, 0.3μm, 0.05μm Al 2 O 3 The powder was polished in turn; then ultrasonically treated with ethanol and ultrapure water for 5 min each; finally, the surface of the electrode was dried with N2 for use; the pretreated bare glassy carbon electrode (GCE) was placed in 2.5% dichromic acid In the mixed solution of potassium and 10% nitric acid, it is oxidized by the potentiostatic method, so that oxygen-containing active groups such as -COOH are generated on the surface. The potential in the potentiostatic ...

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Abstract

Preparation method of label-free electrochemical sensor for cardiac troponin I and its detection method for cTnI. In this experiment, a new type of label-free sensor was constructed by combining electrochemically active Cu-MOF-74 with biological immune reaction. BSA / anti‑cTnI / Cu‑MOF‑74 / NH 2 ‑rGO / GCE sensor, the sensor is specifically reacted with the target molecule (cardiac marker cTnI) to obtain a cardiac marker antigen‑antibody binding layer, which causes electrochemically active Cu‑MOF‑74 layer "electron transfer disturbance", enabling The output electrochemical signal changes regularly. The electrochemical sensor can be used for rapid and sensitive detection of cardiac biomarkers, providing the possibility for early detection, early diagnosis and early treatment of patients with cardiovascular diseases.

Description

technical field [0001] The invention relates to the field of biomedical detection, in particular to a Cu-MOF-74 / NH 2 - Electrochemical sensing technology of label-free cardiac marker cTnI perturbed by electron transfer in rGO composite layer. Background technique [0002] MOFs are porous nanocrystalline materials composed of metal ions and multidentate organic ligands. MOFs have many advantages, such as large specific surface area, high porosity, good thermal stability, and controllable physicochemical properties. Therefore, MOFs have been widely used in many fields such as catalysis, adsorption, optics, electricity, magnetism, and environment. In recent years, based on the unique advantages of MOFs in terms of structure and physicochemical properties, more and more attention has been paid to the research of MOFs in the field of electrochemical analysis, and the application and analysis objects have become wider and wider, involving inorganic ions, organic molecules and bi...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/3277
Inventor 高凤汪庆祥褚亚茹宋娟吴杨仪
Owner MINNAN NORMAL UNIV
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