Use of ferrostatin-1 in the treatment of osteoporosis
A technology for osteoporosis and disease, applied in the field of medicine, can solve problems such as complex development mechanism and incomplete curative effect, achieve the effect of less dosage, expand the application field of disease, and have less side effects
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[0025] 1. Materials
[0026] 1. Experimental animals
[0027] C57 / B6J mice, 15, female, 20 weeks old, weighing 21-37 g, SPF grade, provided by Shanghai Slack Laboratory Animal Co., Ltd. Laboratory animal production license number (SCXK (Shanghai) 2012-0002).
[0028] During the experiment, the temperature of the rearing room was 20±3.2°C, the relative humidity was 65-75%, and the animals had free access to food and water.
[0029] 2. Drugs and reagents:
[0030] 2.1 Drugs
[0031] Sirolimus tablets, trade name Rapa Ming, tablets, 1 mg / tablet, provided by Wyeth Pharmaceuticals (China) Co., Ltd., stored in a refrigerator at 4°C.
[0032] 2.2 Reagents
[0033] Ferrostatin-1, white powder, purity ≥95%, 1 mg / bottle, provided by Sigma-Aldrich Company in the United States, product number is SML0583, stored in a refrigerator at 4°C in the dark.
[0034] β-CTX ELISA kit, provided by China DL Develop Donglin Technology Company, the product number is DL-bCTx-Mu-48T.
[0035] P1NP ...
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