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Near infrared antibiotic fluorescent probe detecting agent and preparing method and application thereof

A technology of fluorescent probes and detection reagents, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of complex design, single detection, and insufficient sensitivity, and achieve simple design, cost saving, and improved sensitivity Effect

Active Publication Date: 2019-08-13
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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  • Abstract
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Problems solved by technology

[0007] Li Zhuang (Sensors and Actuators B 254 (2018) 1118–1124) et al. used the inner filter effect to apply carbon quantum dots and Fe3O4 magnetic nanoparticles to the detection of oxytetracycline. The sensitivity reached 9.5nM, but the sensitivity was not high enough. The design of the system for the detection of oxytetracycline by two nanomaterials is relatively complicated, and it can only detect a single antibiotic

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  • Near infrared antibiotic fluorescent probe detecting agent and preparing method and application thereof
  • Near infrared antibiotic fluorescent probe detecting agent and preparing method and application thereof
  • Near infrared antibiotic fluorescent probe detecting agent and preparing method and application thereof

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preparation example Construction

[0054] In one embodiment, the preparation method of the near-infrared carbon quantum dots comprises: using a microwave-assisted solvent method or a solvothermal method to make a uniformly mixed reaction system containing glutathione, polyethyleneimine and a solvent at 140-180 °C for 1-8 hours to form the near-infrared carbon quantum dots.

[0055] Further, the microwave power used in the microwave-assisted solvent method is 200-400W.

[0056] In the preparation process of near-infrared carbon quantum dots, the regulation of fluorescence spectrum and functional group modification of near-infrared carbon quantum dots can be realized by controlling the reaction time, reaction temperature, and the ratio of reactants.

[0057] In one embodiment, functional groups are distributed on the surface of the fluorescent nanomaterial.

[0058] Further, the functional group includes any one or a combination of two or more of carboxyl, amino, and hydroxyl, but is not limited thereto.

[005...

Embodiment 1

[0093] Example 1 Construction of highly sensitive near-infrared detection fluorescent nanoprobes

[0094] (1) Preparation of polyethyleneimine-modified near-infrared carbon quantum dots:

[0095] Using the common solvothermal method, weigh glutathione and polyethyleneimine (PEI) with a molecular weight of 600 in a beaker, dissolve them with 15 mL of formamide, and mix them uniformly by ultrasonic, wherein glutathione accounts for the mass of formamide The percentage concentration is 3%, and the mass percentage concentration of PEI in glutathione is 30%. After mixing evenly, a transparent solution is obtained. The above solution is transferred to a polytetrafluoroethylene-lined reactor and reacted in an oven at 160°C. 4h, naturally cooled to room temperature. After diluting the reaction mixture with deionized water, it was directly dialyzed with a dialysis bag with a molecular weight cut-off of 3500Da for one week to remove unreacted raw materials and small molecules. Filter ...

Embodiment 2

[0099] Example 2 Construction of highly sensitive near-infrared detection fluorescent nanoprobes

[0100] (1) Preparation of polyethyleneimine-modified near-infrared carbon quantum dots:

[0101] Using the common solvothermal method, weigh glutathione and polyethyleneimine (PEI) with a molecular weight of 600 in a beaker, dissolve them with 15 mL of formamide, and mix them uniformly by ultrasonic, wherein glutathione accounts for the mass of formamide The percentage concentration is 5%, and the mass percentage concentration of PEI accounts for glutathione is 60%. After mixing evenly, a transparent solution is obtained. The above solution is transferred to a polytetrafluoroethylene-lined reaction kettle and placed in an oven at 140°C. Reaction 8h, naturally cooled to room temperature. After diluting the reaction mixture with deionized water, it was directly dialyzed with a dialysis bag with a molecular weight cut-off of 1000 Da for one week to remove unreacted raw materials an...

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Abstract

The invention discloses a near infrared antibiotic fluorescent probe detecting agent and a preparing method and application thereof. The near infrared antibiotic fluorescent probe detecting agent comprises a fluorescent nanometer material. The excitation spectrum of the fluorescent nanometer material and the absorption spectrum of antibiotics are at least partly overlapped so that the excitation wavelength of the fluorescent nanometer material can be absorbed by the antibiotics and the fluorescence of the fluorescent nanometer material can be quenched. The fluorescent nanometer probe agent which is high in selectivity, high in detection sensitivity, simple and feasible and can be used for detecting tetracycline and carbostyril antibiotics is established according to the principle of the internal filtering effect formed between near infrared carbon quantum dots and other fluorescent nanometer materials and the antibiotics, the selectivity of the fluorescent nanometer probe is improved,the technical problem that an existing antibiotic detecting probe is not enough in sensitivity and complicated in detecting process can be solved, the detecting agent has a remarkable effect when usedfor detecting actual samples, and the detecting agent can be easily designed into a sensor for detecting the antibiotics in the actual samples on site.

Description

technical field [0001] The invention relates to a fluorescent nanoprobe reagent for the detection of antibiotics, in particular to a fluorescent nanoprobe for highly sensitive detection of tetracycline and quinolone antibiotics based on near-infrared carbon quantum dots using the principle of inner filter effect and a preparation method thereof , and its application in realizing antibiotic detection, belonging to the technical field of food safety detection. Background technique [0002] Antibiotics, which kill or inhibit the growth of bacteria, have been produced in large quantities around the world over the past few decades and are used to treat various human and veterinary diseases. Although antibiotics have made great contributions to improving the ability of humans to resist diseases, with the extensive use of antibiotics in agriculture and animal husbandry, residues will inevitably form in animal foods and agricultural products. Through the food chain, humans will pas...

Claims

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Application Information

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IPC IPC(8): C09K11/02C09K11/65B82Y40/00G01N21/64
CPCC09K11/02C09K11/65B82Y40/00G01N21/643
Inventor 王宇辉林恒伟乔丽娜
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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