Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Vitrification freezing solution for embryo

A vitrification and freezing liquid technology, applied in the field of assisted reproduction, can solve problems such as hindering low temperature preservation of embryos, harmful nuclei and DNA, and damage to the normal function of the sodium pump, so as to increase the fluidity of the plasma membrane, reduce damage, and improve the quality of blastocysts. formation rate effect

Inactive Publication Date: 2019-08-13
成都艾伟孚生物科技有限公司
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At low temperature, the reduction of its activity will impair the normal function of the sodium pump, resulting in the absorption of sodium ions from the extracellular environment. The accumulation of sodium ions leads to denaturation of intracellular proteins, which is harmful to the nucleus and DNA, and hinders the cryopreservation of embryos.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Vitrification freezing solution for embryo
  • Vitrification freezing solution for embryo
  • Vitrification freezing solution for embryo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The composition of freezing liquid 1 is the whole embryo culture medium + 5% ethylene glycol + 5% DMSO + 0.4mol / L trehalose + 0.1mol / L hydroxypropyl cellulose + 0.5% HSA + 0.6% glycerol;

[0035]The composition of freezing solution 2 is the whole embryo culture medium + 12% ethylene glycol + 12% DMSO + 0.4mol / L trehalose + 0.1mol / L hydroxypropyl cellulose + 0.5% HSA + 0.6% glycerol.

[0036] ① freezing

[0037] 1) Place freezing liquid 1 and freezing liquid 2 at room temperature to equilibrate, and the equilibration time is about 1 hour;

[0038] 2) After the balance is completed, make 200uL of freezing solution 1 and 1 drop of freezing solution 2 in the petri dish;

[0039] 3) Carefully transfer the embryos to the freezing medium to balance for 7 minutes, and minimize the carrying out of the culture medium;

[0040] 4) Carefully transfer the well-balanced embryos in freezing solution 1 to freezing solution 2, and gently blow the embryos to make the embryos sink to th...

Embodiment 2

[0051] The composition of freezing liquid 1 is the whole embryo culture medium + 6% ethylene glycol + 6% DMSO + 0.4mol / L trehalose + 0.2mol / L hydroxypropyl cellulose + 0.4% HSA + 0.6% glycerol;

[0052] The composition of freezing liquid 2 is the whole embryo culture medium + 12% ethylene glycol + 12% DMSO + 0.4 mol / L trehalose + 0.2 mol / L hydroxypropyl cellulose + 0.4% HSA + 0.6% glycerol.

[0053] ① frozen

[0054] 1) Place freezing liquid 1 and freezing liquid 2 at room temperature to equilibrate, and the equilibration time is about 1 hour;

[0055] 2) After the balance is completed, make 200uL of freezing solution 1 and 1 drop of freezing solution 2 in the petri dish;

[0056] 3) Carefully transfer the embryos to the freezing medium to balance for 7 minutes, and minimize the carrying out of the culture medium;

[0057] 4) Carefully transfer the well-balanced embryos in freezing solution 1 to freezing solution 2, and gently blow the embryos to make the embryos sink to the...

Embodiment 3

[0068] The composition of freezing liquid 1 is the whole embryo culture medium + 7% ethylene glycol + 7% DMSO + 0.4mol / L trehalose + 0.3mol / L hydroxypropyl cellulose + 0.4% HSA + 0.5% glycerol;

[0069] The composition of freezing solution 2 is the whole embryo culture medium + 13% ethylene glycol + 13% DMSO + 0.4mol / L trehalose + 0.3mol / L hydroxypropyl cellulose + 0.4% HSA + 0.5% glycerol.

[0070] ① freezing

[0071] 1) Place freezing liquid 1 and freezing liquid 2 at room temperature to equilibrate, and the equilibration time is about 1 hour;

[0072] 2) After the balance is completed, make 200uL of freezing solution 1 and 1 drop of freezing solution 2 in the petri dish;

[0073] 3) Carefully transfer the embryos to the freezing medium to balance for 7 minutes, and minimize the carrying out of the culture medium;

[0074] 4) Carefully transfer the well-balanced embryos in freezing solution 1 to freezing solution 2, and gently blow the embryos to make the embryos sink to t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a vitrification freezing solution for an embryo. According to the vitrification freezing solution for the embryo, the three agents of hydroxypropyl cellulose (HPC), human serumalbumin (HSA) and glycerinum are added to the freezing solution to have a combined effect, and the better capacity of resisting damage is achieved. After the embryo is treated, frozen and stored in the vitrification freezing solution, the blastulation rate of the in-vitro embryo after the embryo is unfrozen can be increased. According to the vitrification freezing solution, the blastulation rateafter the embryo is unfrozen can be greatly increased.

Description

technical field [0001] The invention belongs to the field of assisted reproduction, and in particular relates to an embryo vitrification freezing solution. Background technique [0002] Embryo cryopreservation refers to the use of slow or rapid cooling methods to cryogenically freeze the embryos and then store them in liquid nitrogen at ultra-low temperatures. [0003] The basic principle of embryo vitrification is to solidify a high concentration of cryoprotectant in an ultra-low temperature environment to form an irregular vitrified solid, which preserves the normal distribution of molecules and ions in the liquid state, so it can play a role in vitrification in cells. Protective effects. After the preserved cells are dehydrated to a certain extent in the cryoprotectant solution, the endogenous cytoplasmic macromolecules such as proteins and the cryoprotectant penetrating into the cells are concentrated, so that the cells are protected in the severe and rapid cooling. Cr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 余裕炉左晓玲刘洪君严飞戴甄
Owner 成都艾伟孚生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com