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Preparing method and application of syngnathoides-biaculeatus glycoprotein

A glycoprotein, sea dragon technology, applied in the field of biochemistry, to achieve the effect of convenient and feasible operation, stable structure and convenient operation

Active Publication Date: 2019-08-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chinese Pharmacopoeia records that it has the effects of warming the kidney and strengthening yang, dispelling stagnation and reducing swelling, and has the same medicine and food. Now more and more people use it to make soup, which has a tonic effect. Studies and reports on immune system regulation

Method used

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  • Preparing method and application of syngnathoides-biaculeatus glycoprotein
  • Preparing method and application of syngnathoides-biaculeatus glycoprotein
  • Preparing method and application of syngnathoides-biaculeatus glycoprotein

Examples

Experimental program
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Effect test

Embodiment 1

[0039] A method for preparing a pseudo-sealong glycoprotein, comprising the following steps:

[0040] ①Pretreatment: Wash the pseudo-sea dragon, put it in an oven, dry at 60°C for 4 hours, then pulverize and pass through a 10-mesh sieve to obtain the pseudo-sea dragon coarse powder, and weigh it;

[0041] ② Degreasing treatment: add the pseudo-sea dragon coarse powder obtained in step ① and acetone equivalent to 2 times its volume into a rotary reflux device, extract at 60°C for 2 hours, degrease, then filter, and repeat the rotary reflux extraction for the filter residue once more. The obtained defatted coarse powder is dried;

[0042]③Hot water extraction: Add the degreased coarse powder and distilled water obtained in step ② into a water bath at a ratio of 1:20 (g / mL) to liquid, stir evenly, extract at 100°C for 2.5 hours, filter, and obtain the filtrate for later use. The resulting filter residue was then mixed with distilled water at a solid-liquid ratio of 1:20 (g / mL) f...

Embodiment 2

[0048] The operation of embodiment 2 is basically the same as that of embodiment 1, the difference is:

[0049] In step ③ hot water extraction: add the degreased coarse powder and distilled water obtained in step ② into the water bath at a ratio of 1:25 (g / mL) to liquid, stir evenly, extract at 80°C for 3 hours, filter, and obtain the filtrate Standby, the obtained filter residue is then mixed with distilled water at a solid-liquid ratio of 1:25 (g / mL) and extracted for 1 hour, filtered, and the filtrates combined twice to obtain a mixed solution of pseudo-sealong glycoprotein;

[0050] In step ④ concentrated alcohol precipitation: add the mixed solution obtained in step ③ to a rotary evaporator, evaporate and concentrate at 60°C to 1 / 5 of the original volume, add 3 times the volume of ethanol to the concentrated solution, and stand at 4°C for 12h. Carry out alcohol precipitation, collect the precipitate after centrifuging the alcohol precipitation, and then repeat the alcohol...

Embodiment 3

[0052] The operation of embodiment 3 is basically the same as that of embodiment 1, the difference is:

[0053] In step ③ hot water extraction: add the degreased coarse powder and distilled water obtained in step ② into the water bath at a ratio of 1:30 (g / mL) to liquid, stir evenly, extract at 90°C for 2 hours, filter, and obtain the filtrate Standby, the obtained filter residue is then mixed with distilled water at a solid-liquid ratio of 1:30 (g / mL) for extraction for 1 hour, filtered, and the filtrates combined twice to obtain a mixed solution of pseudo-sealong glycoprotein;

[0054] In step ④ concentrated alcohol precipitation: add the mixed solution obtained in step ③ to a rotary evaporator, evaporate and concentrate at 70°C to 1 / 5 of the original volume, add 3 times the volume of ethanol to the concentrated solution, and stand at 4°C for 16h. Carry out alcohol precipitation, collect the precipitate after centrifuging the alcohol precipitation, and then repeat the alcoho...

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Abstract

The invention relates to the technical field of biochemistry, and discloses a preparing method and application of syngnathoides-biaculeatus glycoprotein. The preparing method includes the steps of pretreatment, ungrease treatment, hot water extraction, concentration alcohol precipitation, washing purification, dialysis freezing drying and the like; the extraction technology of the syngnathoides-biaculeatus glycoprotein is compared and optimized through single factor experiments combined with an orthogonal experiment method, and best preparing technology parameters of the syngnathoides-biaculeatus glycoprotein are obtained. The preparing method of the syngnathoides-biaculeatus glycoprotein has the advantages of being reasonable in design, simple in technology, convenient to operate, high inproduct extraction rate and purity, low in preparing cost, easy to apply and popularize and the like; it also verifies and explains that the syngnathoides-biaculeatus glycoprotein has a remarkable effect in the aspect of the immune adjustment activity through cell experiments, and the theoretical basis and the reference value are provided for application of the syngnathoides-biaculeatus glycoprotein in the immune adjustment aspect and maximum development and utilization of syngnathoides biaculeatus.

Description

technical field [0001] The present invention relates to the technical field of biochemistry, and more specifically, relates to a preparation method and application of a pseudo-sealong glycoprotein. Background technique [0002] Glycoproteins are formed by the covalent bonding of oligosaccharides and polypeptide chains. Glycoproteins widely exist in eukaryotes, and most proteins have glycosylation modifications. Glycoproteins play an important role in organisms and are closely related to the occurrence of some diseases. Sugar chains have the characteristics of micro-heterogeneity and non-template synthesis, which determine the complexity of sugar chain structure and the diversity of composition. Therefore, the analysis of sugar chains has not been able to make a comprehensive and complete structural identification so far. Glycoproteins have biologically active functions such as enhancing immune regulation, anti-tumor, anti-inflammation, lowering blood sugar and blood lipids,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/30C07K1/16C07K1/14A61P37/02
CPCC07K1/36C07K1/34C07K1/30C07K1/16C07K1/145A61P37/02Y02A50/30
Inventor 黄日明杨丽红王宾郝慧丽韩玉
Owner SOUTH CHINA AGRI UNIV
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