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Near infrared fluorescent polymer probe for recognizing hydrazine as well as preparation method and application thereof

A fluorescent polymer and near-infrared technology, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve problems such as damage and limited applications, and achieve the effects of eliminating interference, uniform distribution, and improving accuracy

Active Publication Date: 2019-08-02
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These fluorescent small molecules are easily destroyed by biological enzymes in biological environments, which limits their applications in life sciences, medicine and other fields

Method used

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  • Near infrared fluorescent polymer probe for recognizing hydrazine as well as preparation method and application thereof
  • Near infrared fluorescent polymer probe for recognizing hydrazine as well as preparation method and application thereof
  • Near infrared fluorescent polymer probe for recognizing hydrazine as well as preparation method and application thereof

Examples

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preparation example Construction

[0035] The preparation method of a near-infrared fluorescent polymer probe for identifying hydrazine provided by the present invention comprises the following steps:

[0036] Taking chitosan as the main chain of the polymer, and the near-infrared fluorescent molecule as an activatable fluorescent functional molecule, the amino group of chitosan and the carboxyl group of the near-infrared fluorescent molecule undergo an amidation reaction to introduce chitosan into chitosan, which has the ability to specifically recognize hydrazine. "Turn on" near-infrared fluorescent molecules to obtain near-infrared fluorescent polymer probes that can recognize hydrazine.

[0037] The near-infrared fluorescent molecule used in the present invention has the structure of formula II:

[0038]

[0039] Formula II

[0040] Chitosan is a natural biopolymer with a wide range of sources, safe and non-toxic, and low price. Compared with synthetic polymers, it has good biocompatibility and biodegra...

Embodiment 1

[0041] Example 1 Preparation of near-infrared fluorescent polymer probes for hydrazine recognition

[0042] Dissolve 3.0g chitosan (Mw=10kDa, DA=0.85) in 25mL N,N'-dimethylformamide (DMF), add 5.0g near-infrared fluorescent molecule II and 5mL containing 0.01molN under nitrogen protection -Hydroxysuccinimide (NHS), then 0.04mol of 1-ethyl-(3-dimethylaminopropyl) carbodiimide (EDC) was added, and the reaction was stirred at room temperature for 24h. Dialyzed in deionized water for two days and vacuum freeze-dried to obtain the target probe, which was tested in an ultraviolet absorption spectrometer. figure 1 As shown in the UV-Vis absorption spectrum, the maximum absorption peak of the visible target near-infrared fluorescent probe in aqueous solution is located at 633 nm.

Embodiment 2

[0043] Example 2 Near-infrared fluorescent polymer probes with different equivalents of N 2 H 4 Fluorescence spectral changes of the reaction

[0044] To the probe solution (5 μmol / L) prepared in Example 1, hydrazine solutions of different equivalents (0-3 equiv) were added, mixed evenly, and then shaken for 30 min to perform a fluorescence titration test. Depend on figure 2 It can be seen that with the gradual addition of hydrazine, the fluorescence peak of the probe at 698 nm is gradually enhanced. When the fluorescence intensity reaches the maximum value, it is about 20 times stronger than that of the probe blank solution.

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Abstract

The invention discloses a near infrared fluorescent polymer probe for recognizing hydrazine as well as a preparation method and application thereof, and in particular discloses the near infrared fluorescent polymer probe of a structure as shown in a formula I. The polymer probe is prepared by carrying out a chemical reaction on near infrared fluorescent molecules and biocompatible chitosan, wherein the near infrared fluorescent molecules, as a near infrared fluorescent response unit, can react with hydrazine to emit red light so as to achieve specific response to hydrazine. Chitosan, as a mainchain composition unit of the polymer, endows the polymer with good water solubility and biocompatibility. The near infrared fluorescent polymer probe provided by the invention can be used for hydrazine detection and imaging in an aqueous solution, living cells and a living body, and has an important application prospect in the fields of environment monitoring, bioimaging and sensing. The formulaI is as shown in the description.

Description

technical field [0001] The invention belongs to the field of polymer materials and chemical sensors, and particularly relates to a near-infrared fluorescent polymer probe for identifying hydrazine, a preparation method and application thereof. Background technique [0002] Hydrazine (N 2 H 4 ), also known as hydrazine, is a chemical raw material with strong reducibility, which is widely used in industrial production and can be used to produce preservatives, antioxidants, herbicides and plant growth regulators. In addition, N 2 H 4 It has a high heat of combustion and can be used as fuel for rockets and fuel cells. However N 2 H 4 It is a toxic substance, and its leakage during production, transportation and application will cause pollution to the environment. There is no endogenous hydrazine in living cells, but hydrazine can be absorbed by the body through breathing or skin contact, causing severe damage to the body's liver, lungs, kidneys, and central nervous system...

Claims

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Application Information

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IPC IPC(8): C08B37/08C09K11/06G01N21/64
CPCC08B37/003C09K11/06C09K2211/1441G01N21/6428
Inventor 张怀红周桃于琴杨正莹仓辉蔡照胜
Owner YANCHENG INST OF TECH
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